Amino acid dipepetide frequency for Stenotrophomonas phage IME-SM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.684AlaAla: 6.684 ± 0.521
0.668AlaCys: 0.668 ± 0.137
5.037AlaAsp: 5.037 ± 0.399
5.157AlaGlu: 5.157 ± 0.466
2.96AlaPhe: 2.96 ± 0.283
5.539AlaGly: 5.539 ± 0.442
1.074AlaHis: 1.074 ± 0.143
5.013AlaIle: 5.013 ± 0.334
3.82AlaLys: 3.82 ± 0.319
6.947AlaLeu: 6.947 ± 0.458
2.005AlaMet: 2.005 ± 0.206
3.462AlaAsn: 3.462 ± 0.317
3.08AlaPro: 3.08 ± 0.361
2.889AlaGln: 2.889 ± 0.305
4.273AlaArg: 4.273 ± 0.321
4.87AlaSer: 4.87 ± 0.356
4.512AlaThr: 4.512 ± 0.358
4.799AlaVal: 4.799 ± 0.287
1.409AlaTrp: 1.409 ± 0.177
2.793AlaTyr: 2.793 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.573CysAla: 0.573 ± 0.108
0.167CysCys: 0.167 ± 0.064
0.621CysAsp: 0.621 ± 0.118
0.573CysGlu: 0.573 ± 0.108
0.31CysPhe: 0.31 ± 0.093
0.573CysGly: 0.573 ± 0.137
0.358CysHis: 0.358 ± 0.093
0.573CysIle: 0.573 ± 0.121
0.477CysLys: 0.477 ± 0.094
0.716CysLeu: 0.716 ± 0.138
0.501CysMet: 0.501 ± 0.115
0.477CysAsn: 0.477 ± 0.103
0.621CysPro: 0.621 ± 0.13
0.43CysGln: 0.43 ± 0.096
0.525CysArg: 0.525 ± 0.102
0.764CysSer: 0.764 ± 0.144
0.692CysThr: 0.692 ± 0.111
0.692CysVal: 0.692 ± 0.145
0.263CysTrp: 0.263 ± 0.092
0.358CysTyr: 0.358 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
5.753AspAla: 5.753 ± 0.497
0.573AspCys: 0.573 ± 0.105
4.297AspAsp: 4.297 ± 0.348
4.536AspGlu: 4.536 ± 0.414
3.223AspPhe: 3.223 ± 0.278
4.942AspGly: 4.942 ± 0.339
0.907AspHis: 0.907 ± 0.13
3.223AspIle: 3.223 ± 0.286
3.676AspLys: 3.676 ± 0.387
6.088AspLeu: 6.088 ± 0.399
1.958AspMet: 1.958 ± 0.246
2.101AspAsn: 2.101 ± 0.233
3.724AspPro: 3.724 ± 0.259
2.626AspGln: 2.626 ± 0.298
3.199AspArg: 3.199 ± 0.29
3.987AspSer: 3.987 ± 0.326
3.485AspThr: 3.485 ± 0.35
4.417AspVal: 4.417 ± 0.341
1.17AspTrp: 1.17 ± 0.151
2.531AspTyr: 2.531 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
5.348GluAla: 5.348 ± 0.429
0.645GluCys: 0.645 ± 0.132
3.867GluAsp: 3.867 ± 0.363
4.536GluGlu: 4.536 ± 0.405
3.008GluPhe: 3.008 ± 0.271
4.011GluGly: 4.011 ± 0.348
1.289GluHis: 1.289 ± 0.16
4.393GluIle: 4.393 ± 0.338
3.796GluLys: 3.796 ± 0.33
6.422GluLeu: 6.422 ± 0.516
1.886GluMet: 1.886 ± 0.268
3.08GluAsn: 3.08 ± 0.278
2.029GluPro: 2.029 ± 0.263
2.554GluGln: 2.554 ± 0.255
3.271GluArg: 3.271 ± 0.269
3.414GluSer: 3.414 ± 0.314
3.223GluThr: 3.223 ± 0.247
4.297GluVal: 4.297 ± 0.337
1.409GluTrp: 1.409 ± 0.167
2.841GluTyr: 2.841 ± 0.256
0.0GluXaa: 0.0 ± 0.0
Phe
2.65PheAla: 2.65 ± 0.279
0.43PheCys: 0.43 ± 0.088
3.748PheAsp: 3.748 ± 0.363
2.101PheGlu: 2.101 ± 0.199
1.6PhePhe: 1.6 ± 0.197
3.032PheGly: 3.032 ± 0.317
0.812PheHis: 0.812 ± 0.134
2.626PheIle: 2.626 ± 0.231
3.104PheLys: 3.104 ± 0.303
2.769PheLeu: 2.769 ± 0.241
1.265PheMet: 1.265 ± 0.177
2.172PheAsn: 2.172 ± 0.208
1.361PhePro: 1.361 ± 0.183
1.934PheGln: 1.934 ± 0.215
2.531PheArg: 2.531 ± 0.272
2.316PheSer: 2.316 ± 0.211
2.578PheThr: 2.578 ± 0.201
2.793PheVal: 2.793 ± 0.249
0.716PheTrp: 0.716 ± 0.144
1.647PheTyr: 1.647 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
5.133GlyAla: 5.133 ± 0.461
0.764GlyCys: 0.764 ± 0.133
3.653GlyAsp: 3.653 ± 0.267
4.417GlyGlu: 4.417 ± 0.389
2.769GlyPhe: 2.769 ± 0.266
4.727GlyGly: 4.727 ± 0.349
1.098GlyHis: 1.098 ± 0.197
3.414GlyIle: 3.414 ± 0.335
4.488GlyLys: 4.488 ± 0.319
4.942GlyLeu: 4.942 ± 0.339
1.958GlyMet: 1.958 ± 0.197
3.175GlyAsn: 3.175 ± 0.336
1.695GlyPro: 1.695 ± 0.182
2.841GlyGln: 2.841 ± 0.266
2.745GlyArg: 2.745 ± 0.254
4.178GlySer: 4.178 ± 0.336
5.037GlyThr: 5.037 ± 0.48
4.846GlyVal: 4.846 ± 0.377
1.504GlyTrp: 1.504 ± 0.167
3.008GlyTyr: 3.008 ± 0.291
0.0GlyXaa: 0.0 ± 0.0
His
1.265HisAla: 1.265 ± 0.18
0.191HisCys: 0.191 ± 0.069
1.146HisAsp: 1.146 ± 0.181
0.955HisGlu: 0.955 ± 0.18
0.931HisPhe: 0.931 ± 0.185
1.337HisGly: 1.337 ± 0.176
0.477HisHis: 0.477 ± 0.118
0.907HisIle: 0.907 ± 0.151
1.122HisLys: 1.122 ± 0.172
1.313HisLeu: 1.313 ± 0.179
0.477HisMet: 0.477 ± 0.126
0.788HisAsn: 0.788 ± 0.136
0.788HisPro: 0.788 ± 0.139
0.549HisGln: 0.549 ± 0.094
1.361HisArg: 1.361 ± 0.185
0.836HisSer: 0.836 ± 0.128
0.883HisThr: 0.883 ± 0.15
1.218HisVal: 1.218 ± 0.146
0.143HisTrp: 0.143 ± 0.054
0.597HisTyr: 0.597 ± 0.098
0.0HisXaa: 0.0 ± 0.0
Ile
4.584IleAla: 4.584 ± 0.362
0.549IleCys: 0.549 ± 0.107
3.963IleAsp: 3.963 ± 0.348
4.488IleGlu: 4.488 ± 0.325
1.838IlePhe: 1.838 ± 0.179
3.82IleGly: 3.82 ± 0.321
1.003IleHis: 1.003 ± 0.161
2.841IleIle: 2.841 ± 0.326
4.727IleLys: 4.727 ± 0.312
4.393IleLeu: 4.393 ± 0.346
1.218IleMet: 1.218 ± 0.16
2.745IleAsn: 2.745 ± 0.311
3.223IlePro: 3.223 ± 0.307
2.531IleGln: 2.531 ± 0.278
3.629IleArg: 3.629 ± 0.291
3.223IleSer: 3.223 ± 0.289
4.13IleThr: 4.13 ± 0.299
3.891IleVal: 3.891 ± 0.271
0.716IleTrp: 0.716 ± 0.124
2.053IleTyr: 2.053 ± 0.213
0.0IleXaa: 0.0 ± 0.0
Lys
4.918LysAla: 4.918 ± 0.335
0.501LysCys: 0.501 ± 0.116
3.438LysAsp: 3.438 ± 0.306
3.581LysGlu: 3.581 ± 0.327
2.387LysPhe: 2.387 ± 0.24
3.342LysGly: 3.342 ± 0.35
1.146LysHis: 1.146 ± 0.164
4.369LysIle: 4.369 ± 0.306
4.178LysLys: 4.178 ± 0.343
5.324LysLeu: 5.324 ± 0.383
2.244LysMet: 2.244 ± 0.246
2.674LysAsn: 2.674 ± 0.258
2.626LysPro: 2.626 ± 0.291
2.268LysGln: 2.268 ± 0.267
3.509LysArg: 3.509 ± 0.309
3.485LysSer: 3.485 ± 0.276
3.318LysThr: 3.318 ± 0.277
4.058LysVal: 4.058 ± 0.329
1.194LysTrp: 1.194 ± 0.187
2.602LysTyr: 2.602 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
5.658LeuAla: 5.658 ± 0.363
0.788LeuCys: 0.788 ± 0.142
6.35LeuAsp: 6.35 ± 0.393
6.326LeuGlu: 6.326 ± 0.45
2.817LeuPhe: 2.817 ± 0.271
4.608LeuGly: 4.608 ± 0.342
1.623LeuHis: 1.623 ± 0.215
4.727LeuIle: 4.727 ± 0.253
5.204LeuLys: 5.204 ± 0.444
5.371LeuLeu: 5.371 ± 0.356
2.22LeuMet: 2.22 ± 0.238
4.655LeuAsn: 4.655 ± 0.336
3.056LeuPro: 3.056 ± 0.332
3.39LeuGln: 3.39 ± 0.302
4.106LeuArg: 4.106 ± 0.309
5.085LeuSer: 5.085 ± 0.409
5.061LeuThr: 5.061 ± 0.382
5.013LeuVal: 5.013 ± 0.338
1.098LeuTrp: 1.098 ± 0.145
2.531LeuTyr: 2.531 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
2.149MetAla: 2.149 ± 0.25
0.31MetCys: 0.31 ± 0.085
1.934MetAsp: 1.934 ± 0.198
1.623MetGlu: 1.623 ± 0.196
1.194MetPhe: 1.194 ± 0.174
1.385MetGly: 1.385 ± 0.214
0.43MetHis: 0.43 ± 0.102
1.48MetIle: 1.48 ± 0.195
1.79MetLys: 1.79 ± 0.244
1.934MetLeu: 1.934 ± 0.21
0.883MetMet: 0.883 ± 0.167
1.862MetAsn: 1.862 ± 0.216
1.098MetPro: 1.098 ± 0.163
1.194MetGln: 1.194 ± 0.188
1.313MetArg: 1.313 ± 0.183
2.172MetSer: 2.172 ± 0.232
1.981MetThr: 1.981 ± 0.26
1.385MetVal: 1.385 ± 0.167
0.286MetTrp: 0.286 ± 0.075
1.194MetTyr: 1.194 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
3.438AsnAla: 3.438 ± 0.304
0.501AsnCys: 0.501 ± 0.105
2.65AsnAsp: 2.65 ± 0.285
2.722AsnGlu: 2.722 ± 0.246
2.411AsnPhe: 2.411 ± 0.304
4.297AsnGly: 4.297 ± 0.398
0.549AsnHis: 0.549 ± 0.129
3.008AsnIle: 3.008 ± 0.249
3.032AsnLys: 3.032 ± 0.335
3.748AsnLeu: 3.748 ± 0.248
1.194AsnMet: 1.194 ± 0.155
2.244AsnAsn: 2.244 ± 0.298
2.554AsnPro: 2.554 ± 0.206
1.862AsnGln: 1.862 ± 0.256
2.65AsnArg: 2.65 ± 0.217
2.769AsnSer: 2.769 ± 0.248
2.745AsnThr: 2.745 ± 0.28
2.841AsnVal: 2.841 ± 0.288
0.812AsnTrp: 0.812 ± 0.157
1.504AsnTyr: 1.504 ± 0.231
0.0AsnXaa: 0.0 ± 0.0
Pro
3.318ProAla: 3.318 ± 0.323
0.406ProCys: 0.406 ± 0.108
3.318ProAsp: 3.318 ± 0.272
2.769ProGlu: 2.769 ± 0.233
1.958ProPhe: 1.958 ± 0.207
2.913ProGly: 2.913 ± 0.301
0.406ProHis: 0.406 ± 0.108
2.22ProIle: 2.22 ± 0.225
2.292ProLys: 2.292 ± 0.22
2.698ProLeu: 2.698 ± 0.258
0.812ProMet: 0.812 ± 0.14
2.029ProAsn: 2.029 ± 0.244
1.528ProPro: 1.528 ± 0.22
1.456ProGln: 1.456 ± 0.188
1.647ProArg: 1.647 ± 0.189
2.125ProSer: 2.125 ± 0.242
3.032ProThr: 3.032 ± 0.374
3.414ProVal: 3.414 ± 0.312
0.454ProTrp: 0.454 ± 0.104
1.886ProTyr: 1.886 ± 0.224
0.0ProXaa: 0.0 ± 0.0
Gln
3.271GlnAla: 3.271 ± 0.311
0.454GlnCys: 0.454 ± 0.118
2.172GlnAsp: 2.172 ± 0.197
2.387GlnGlu: 2.387 ± 0.274
2.125GlnPhe: 2.125 ± 0.208
2.172GlnGly: 2.172 ± 0.249
0.573GlnHis: 0.573 ± 0.122
3.104GlnIle: 3.104 ± 0.257
2.029GlnLys: 2.029 ± 0.22
3.366GlnLeu: 3.366 ± 0.283
1.409GlnMet: 1.409 ± 0.204
1.886GlnAsn: 1.886 ± 0.184
1.289GlnPro: 1.289 ± 0.19
1.719GlnGln: 1.719 ± 0.236
2.292GlnArg: 2.292 ± 0.251
2.22GlnSer: 2.22 ± 0.262
2.507GlnThr: 2.507 ± 0.284
2.913GlnVal: 2.913 ± 0.313
0.692GlnTrp: 0.692 ± 0.146
1.576GlnTyr: 1.576 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
3.844ArgAla: 3.844 ± 0.304
0.764ArgCys: 0.764 ± 0.14
3.82ArgAsp: 3.82 ± 0.283
3.557ArgGlu: 3.557 ± 0.35
2.459ArgPhe: 2.459 ± 0.239
3.342ArgGly: 3.342 ± 0.379
0.764ArgHis: 0.764 ± 0.157
3.247ArgIle: 3.247 ± 0.274
2.913ArgLys: 2.913 ± 0.27
4.417ArgLeu: 4.417 ± 0.352
1.241ArgMet: 1.241 ± 0.203
2.268ArgAsn: 2.268 ± 0.265
1.79ArgPro: 1.79 ± 0.236
2.363ArgGln: 2.363 ± 0.224
3.414ArgArg: 3.414 ± 0.322
3.151ArgSer: 3.151 ± 0.297
2.698ArgThr: 2.698 ± 0.27
3.318ArgVal: 3.318 ± 0.26
0.931ArgTrp: 0.931 ± 0.168
2.316ArgTyr: 2.316 ± 0.272
0.0ArgXaa: 0.0 ± 0.0
Ser
4.345SerAla: 4.345 ± 0.322
0.716SerCys: 0.716 ± 0.144
3.796SerAsp: 3.796 ± 0.24
3.891SerGlu: 3.891 ± 0.302
2.817SerPhe: 2.817 ± 0.348
4.679SerGly: 4.679 ± 0.323
0.955SerHis: 0.955 ± 0.155
3.485SerIle: 3.485 ± 0.324
3.581SerLys: 3.581 ± 0.325
4.822SerLeu: 4.822 ± 0.385
1.361SerMet: 1.361 ± 0.169
2.483SerAsn: 2.483 ± 0.207
2.387SerPro: 2.387 ± 0.297
2.602SerGln: 2.602 ± 0.261
3.08SerArg: 3.08 ± 0.241
4.321SerSer: 4.321 ± 0.383
3.987SerThr: 3.987 ± 0.361
4.345SerVal: 4.345 ± 0.291
1.074SerTrp: 1.074 ± 0.167
2.268SerTyr: 2.268 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
5.419ThrAla: 5.419 ± 0.436
0.692ThrCys: 0.692 ± 0.115
3.748ThrAsp: 3.748 ± 0.289
3.485ThrGlu: 3.485 ± 0.298
2.913ThrPhe: 2.913 ± 0.31
4.727ThrGly: 4.727 ± 0.521
0.979ThrHis: 0.979 ± 0.187
3.509ThrIle: 3.509 ± 0.267
3.175ThrLys: 3.175 ± 0.263
5.037ThrLeu: 5.037 ± 0.349
1.695ThrMet: 1.695 ± 0.231
2.841ThrAsn: 2.841 ± 0.333
3.175ThrPro: 3.175 ± 0.299
2.029ThrGln: 2.029 ± 0.205
2.554ThrArg: 2.554 ± 0.205
4.106ThrSer: 4.106 ± 0.43
3.438ThrThr: 3.438 ± 0.388
4.87ThrVal: 4.87 ± 0.409
1.122ThrTrp: 1.122 ± 0.182
2.602ThrTyr: 2.602 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
4.488ValAla: 4.488 ± 0.331
0.692ValCys: 0.692 ± 0.122
5.157ValAsp: 5.157 ± 0.375
4.655ValGlu: 4.655 ± 0.329
2.387ValPhe: 2.387 ± 0.237
4.154ValGly: 4.154 ± 0.396
1.48ValHis: 1.48 ± 0.19
4.345ValIle: 4.345 ± 0.322
4.44ValLys: 4.44 ± 0.307
4.799ValLeu: 4.799 ± 0.329
1.671ValMet: 1.671 ± 0.176
3.294ValAsn: 3.294 ± 0.287
2.889ValPro: 2.889 ± 0.294
2.483ValGln: 2.483 ± 0.216
3.509ValArg: 3.509 ± 0.305
4.297ValSer: 4.297 ± 0.343
5.013ValThr: 5.013 ± 0.392
4.846ValVal: 4.846 ± 0.355
0.812ValTrp: 0.812 ± 0.122
2.149ValTyr: 2.149 ± 0.236
0.0ValXaa: 0.0 ± 0.0
Trp
1.241TrpAla: 1.241 ± 0.225
0.072TrpCys: 0.072 ± 0.043
0.836TrpAsp: 0.836 ± 0.147
1.003TrpGlu: 1.003 ± 0.16
0.74TrpPhe: 0.74 ± 0.15
0.955TrpGly: 0.955 ± 0.161
0.43TrpHis: 0.43 ± 0.114
0.931TrpIle: 0.931 ± 0.151
0.979TrpLys: 0.979 ± 0.164
1.48TrpLeu: 1.48 ± 0.17
0.454TrpMet: 0.454 ± 0.118
1.098TrpAsn: 1.098 ± 0.151
0.215TrpPro: 0.215 ± 0.07
0.573TrpGln: 0.573 ± 0.127
1.074TrpArg: 1.074 ± 0.165
1.194TrpSer: 1.194 ± 0.175
1.122TrpThr: 1.122 ± 0.137
1.027TrpVal: 1.027 ± 0.149
0.263TrpTrp: 0.263 ± 0.062
0.931TrpTyr: 0.931 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.913TyrAla: 2.913 ± 0.276
0.382TyrCys: 0.382 ± 0.103
2.865TyrAsp: 2.865 ± 0.267
2.554TyrGlu: 2.554 ± 0.297
1.337TyrPhe: 1.337 ± 0.19
1.838TyrGly: 1.838 ± 0.187
0.931TyrHis: 0.931 ± 0.149
2.149TyrIle: 2.149 ± 0.229
2.34TyrLys: 2.34 ± 0.231
3.104TyrLeu: 3.104 ± 0.281
1.122TyrMet: 1.122 ± 0.172
2.22TyrAsn: 2.22 ± 0.221
1.552TyrPro: 1.552 ± 0.157
1.862TyrGln: 1.862 ± 0.228
1.934TyrArg: 1.934 ± 0.244
2.483TyrSer: 2.483 ± 0.272
2.674TyrThr: 2.674 ± 0.294
2.578TyrVal: 2.578 ± 0.304
0.549TyrTrp: 0.549 ± 0.097
1.48TyrTyr: 1.48 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 202 proteins (41889 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski