Autographa californica nuclear polyhedrosis virus (AcMNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus; Autographa californica multiple nucleopolyhedrovirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P41659|Y106_NPVAC Uncharacterized 7.1 kDa protein in HE65-PK2 intergenic region OS=Autographa californica nuclear polyhedrosis virus OX=46015 PE=4 SV=1
MM1 pKa = 7.26ATWICWPNNAYY12 pKa = 9.52IDD14 pKa = 3.8ACTFIVVIILIHH26 pKa = 6.68LIEE29 pKa = 4.98LNIHH33 pKa = 5.36LQWVKK38 pKa = 10.92EE39 pKa = 4.09SLNFAMEE46 pKa = 4.4NGDD49 pKa = 5.77KK50 pKa = 10.69EE51 pKa = 5.25DD52 pKa = 4.17SDD54 pKa = 4.71NEE56 pKa = 4.1

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P24731|AN_NPVAC Alkaline nuclease OS=Autographa californica nuclear polyhedrosis virus OX=46015 GN=AN PE=1 SV=1
MM1 pKa = 7.57KK2 pKa = 8.07PTNNVMFDD10 pKa = 3.7DD11 pKa = 6.11ASVLWIDD18 pKa = 3.72TDD20 pKa = 4.41YY21 pKa = 11.02IYY23 pKa = 11.21QNLKK27 pKa = 9.57MPLQAFQQLLFTIPSKK43 pKa = 10.03HH44 pKa = 5.88RR45 pKa = 11.84KK46 pKa = 7.82MINDD50 pKa = 3.71AGGSCHH56 pKa = 5.81NTVKK60 pKa = 10.97YY61 pKa = 9.87MVDD64 pKa = 2.77IYY66 pKa = 11.18GAAVLVLRR74 pKa = 11.84TPCSFADD81 pKa = 3.49QLLSTFIANNYY92 pKa = 8.53LCYY95 pKa = 9.83FYY97 pKa = 10.79RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84SRR103 pKa = 11.84SRR105 pKa = 11.84SRR107 pKa = 11.84SRR109 pKa = 11.84SRR111 pKa = 11.84SRR113 pKa = 11.84SPHH116 pKa = 5.16CRR118 pKa = 11.84PRR120 pKa = 11.84SRR122 pKa = 11.84SPHH125 pKa = 5.28CRR127 pKa = 11.84PRR129 pKa = 11.84SRR131 pKa = 11.84SRR133 pKa = 11.84SRR135 pKa = 11.84SRR137 pKa = 11.84SRR139 pKa = 11.84SRR141 pKa = 11.84SSSPRR146 pKa = 11.84RR147 pKa = 11.84GRR149 pKa = 11.84RR150 pKa = 11.84QIFDD154 pKa = 3.45ALEE157 pKa = 4.5KK158 pKa = 10.15IRR160 pKa = 11.84HH161 pKa = 5.15QNDD164 pKa = 2.9MLMSNVNQINLNQTNQFLEE183 pKa = 4.64LSNMMTGVRR192 pKa = 11.84NQNVQLLAALEE203 pKa = 4.14TAKK206 pKa = 10.87DD207 pKa = 3.83VILTRR212 pKa = 11.84LNTLLAEE219 pKa = 4.59ITDD222 pKa = 4.18SLPDD226 pKa = 3.73LTSMLDD232 pKa = 3.42KK233 pKa = 10.81LAEE236 pKa = 4.0QLLDD240 pKa = 4.78AINTVQQTCATSS252 pKa = 3.01

Molecular weight: 29.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

155

0

155

41208

53

1221

265.9

30.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.2 ± 0.169

2.524 ± 0.145

6.035 ± 0.156

5.322 ± 0.149

4.972 ± 0.119

3.276 ± 0.164

2.252 ± 0.101

6.557 ± 0.125

7.19 ± 0.252

9.282 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.082

7.894 ± 0.17

3.946 ± 0.26

3.808 ± 0.149

4.642 ± 0.155

6.317 ± 0.167

5.647 ± 0.151

6.686 ± 0.147

0.772 ± 0.068

4.924 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski