Enteractinococcus helveticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Enteractinococcus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B7LXW3|A0A1B7LXW3_9MICC Uncharacterized protein OS=Enteractinococcus helveticum OX=1837282 GN=A6F49_12085 PE=4 SV=1
MM1 pKa = 7.62SKK3 pKa = 10.27PIKK6 pKa = 10.16QYY8 pKa = 10.02WPIAATLGLIAVIVLALLRR27 pKa = 11.84PWSTPKK33 pKa = 10.29TEE35 pKa = 4.41LTFEE39 pKa = 4.66LSPTQVDD46 pKa = 3.45IAIDD50 pKa = 3.73SQDD53 pKa = 3.7YY54 pKa = 10.78GSVASGEE61 pKa = 4.09MLEE64 pKa = 4.51VEE66 pKa = 4.48LSGDD70 pKa = 3.93VIVEE74 pKa = 3.98ASRR77 pKa = 11.84EE78 pKa = 4.17GFTPEE83 pKa = 4.25SLPLTVDD90 pKa = 2.99PGEE93 pKa = 4.21AQTVYY98 pKa = 10.67FGLVPEE104 pKa = 4.41TPEE107 pKa = 4.22AEE109 pKa = 4.18EE110 pKa = 5.26LVDD113 pKa = 3.89EE114 pKa = 4.32QDD116 pKa = 3.66SVVDD120 pKa = 3.71EE121 pKa = 4.06QVITEE126 pKa = 4.38EE127 pKa = 3.92YY128 pKa = 9.59HH129 pKa = 7.4DD130 pKa = 3.98RR131 pKa = 11.84AEE133 pKa = 4.47GMFDD137 pKa = 3.12KK138 pKa = 11.05HH139 pKa = 6.74PILSDD144 pKa = 3.86LPQEE148 pKa = 4.19DD149 pKa = 4.21QYY151 pKa = 11.97YY152 pKa = 10.49GAYY155 pKa = 9.69QGIPEE160 pKa = 4.5GDD162 pKa = 3.07AHH164 pKa = 7.14DD165 pKa = 4.24FAIHH169 pKa = 5.41LHH171 pKa = 6.45LYY173 pKa = 9.31VGHH176 pKa = 6.51EE177 pKa = 3.92AQGRR181 pKa = 11.84EE182 pKa = 4.13DD183 pKa = 3.91FEE185 pKa = 4.62VWMDD189 pKa = 3.65DD190 pKa = 3.69AGYY193 pKa = 8.23STSNYY198 pKa = 10.16QIIEE202 pKa = 4.54DD203 pKa = 4.5IKK205 pKa = 11.52DD206 pKa = 3.45EE207 pKa = 4.77DD208 pKa = 4.31PPVIPDD214 pKa = 4.54DD215 pKa = 4.53GPSYY219 pKa = 9.9TALQDD224 pKa = 3.7MNPGDD229 pKa = 3.81ISMPTADD236 pKa = 3.99NKK238 pKa = 11.26GLGVDD243 pKa = 3.97ALALHH248 pKa = 6.48FAEE251 pKa = 6.35VSTTWDD257 pKa = 3.1TAEE260 pKa = 4.92DD261 pKa = 3.63VHH263 pKa = 6.47HH264 pKa = 7.01TDD266 pKa = 2.84GLIRR270 pKa = 11.84AKK272 pKa = 10.55PLMTHH277 pKa = 7.21DD278 pKa = 4.42LADD281 pKa = 4.49SIEE284 pKa = 4.34MPHH287 pKa = 6.87RR288 pKa = 11.84PTPSQSWWTAYY299 pKa = 7.59EE300 pKa = 4.29HH301 pKa = 6.17EE302 pKa = 4.66AKK304 pKa = 10.58SYY306 pKa = 10.5VWLHH310 pKa = 6.97DD311 pKa = 3.65YY312 pKa = 11.01SSQHH316 pKa = 5.59YY317 pKa = 9.23RR318 pKa = 11.84DD319 pKa = 3.54NSTVEE324 pKa = 4.15MQVCWAWVADD334 pKa = 3.77EE335 pKa = 4.78MEE337 pKa = 4.51PVVDD341 pKa = 5.05GPRR344 pKa = 11.84TLEE347 pKa = 3.94ATVDD351 pKa = 3.58TTGSDD356 pKa = 3.61NIISDD361 pKa = 4.26FFYY364 pKa = 10.73EE365 pKa = 4.87DD366 pKa = 3.79PDD368 pKa = 4.43DD369 pKa = 5.07FVDD372 pKa = 5.29SSASPCIPEE381 pKa = 4.48DD382 pKa = 3.46ASS384 pKa = 3.41

Molecular weight: 42.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B7LWN3|A0A1B7LWN3_9MICC Amino_oxidase domain-containing protein OS=Enteractinococcus helveticum OX=1837282 GN=A6F49_16625 PE=4 SV=1
MM1 pKa = 7.72ASNHH5 pKa = 5.14NRR7 pKa = 11.84SVGAFFAGVFMVNSLGHH24 pKa = 6.53FATAIAGKK32 pKa = 8.45QHH34 pKa = 6.47LTPLAGRR41 pKa = 11.84RR42 pKa = 11.84SGPAVNALWGAMNLAGGLALARR64 pKa = 11.84RR65 pKa = 4.28

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

1011829

55

2016

316.1

34.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.315 ± 0.045

0.508 ± 0.009

6.169 ± 0.048

6.131 ± 0.048

3.319 ± 0.025

7.854 ± 0.037

2.445 ± 0.024

5.453 ± 0.034

2.747 ± 0.032

9.713 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.02

2.859 ± 0.025

4.978 ± 0.032

4.095 ± 0.03

5.899 ± 0.042

5.706 ± 0.032

6.631 ± 0.034

7.973 ± 0.033

1.471 ± 0.019

2.373 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski