Wenxinia saemankumensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Wenxinia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6A3K7|A0A1M6A3K7_9RHOB Peptide/nickel transport system permease protein OS=Wenxinia saemankumensis OX=1447782 GN=SAMN05444417_0183 PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.53KK3 pKa = 10.38LLLSSALTLLAAPALAQDD21 pKa = 4.74GEE23 pKa = 4.53CGEE26 pKa = 4.23VSITEE31 pKa = 4.25MNWASASVVTNVASFILTQGYY52 pKa = 8.92GCDD55 pKa = 3.59VSIVPSDD62 pKa = 3.71TVPAITSVSEE72 pKa = 3.85NGEE75 pKa = 3.85PDD77 pKa = 3.08IVTEE81 pKa = 4.49LWTNATGQVYY91 pKa = 10.34EE92 pKa = 4.14EE93 pKa = 4.51MKK95 pKa = 10.82DD96 pKa = 3.08AGTIQEE102 pKa = 4.55LGAVLEE108 pKa = 4.68PGGIDD113 pKa = 3.37AWWIPAYY120 pKa = 10.25LAEE123 pKa = 4.47AHH125 pKa = 6.96PEE127 pKa = 3.78LTTIEE132 pKa = 4.29GVLANPDD139 pKa = 3.37LVGGRR144 pKa = 11.84FHH146 pKa = 7.13NCPEE150 pKa = 3.77GWGCRR155 pKa = 11.84VFNDD159 pKa = 3.29NLLPAFGVEE168 pKa = 3.93EE169 pKa = 4.09AGIEE173 pKa = 4.12IFDD176 pKa = 4.18HH177 pKa = 7.12GSGEE181 pKa = 4.23TLATSIASAFEE192 pKa = 4.23NEE194 pKa = 4.51EE195 pKa = 3.93PWFGYY200 pKa = 9.3YY201 pKa = 8.43WAPTSVIGRR210 pKa = 11.84YY211 pKa = 10.55DD212 pKa = 3.46MVQVDD217 pKa = 3.4MGDD220 pKa = 3.76YY221 pKa = 10.74DD222 pKa = 4.62RR223 pKa = 11.84EE224 pKa = 4.25AHH226 pKa = 6.35EE227 pKa = 4.45AAQNVDD233 pKa = 3.05ATDD236 pKa = 3.3VGRR239 pKa = 11.84TSFPPAPVATAVTTDD254 pKa = 3.55FAEE257 pKa = 4.28AHH259 pKa = 6.58PEE261 pKa = 3.92LVDD264 pKa = 3.8FLSNMTFQTDD274 pKa = 3.28TMNSILSWMEE284 pKa = 3.27EE285 pKa = 3.71NAASGEE291 pKa = 4.06EE292 pKa = 4.44GAVHH296 pKa = 7.41FLTTYY301 pKa = 8.58QDD303 pKa = 3.14EE304 pKa = 4.29WRR306 pKa = 11.84GWISGEE312 pKa = 3.8ARR314 pKa = 11.84EE315 pKa = 4.61NLSAMLQQQ323 pKa = 4.01

Molecular weight: 34.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6E9S4|A0A1M6E9S4_9RHOB 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Wenxinia saemankumensis OX=1447782 GN=ispE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25SGRR28 pKa = 11.84QIIAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3386

0

3386

1058228

29

2118

312.5

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.892 ± 0.069

0.787 ± 0.013

6.143 ± 0.035

6.123 ± 0.039

3.346 ± 0.026

9.661 ± 0.049

1.928 ± 0.021

4.615 ± 0.031

1.963 ± 0.038

10.156 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.02

1.945 ± 0.022

5.814 ± 0.042

2.626 ± 0.027

7.983 ± 0.046

4.505 ± 0.023

5.246 ± 0.031

7.244 ± 0.032

1.506 ± 0.019

2.0 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski