Mycobacterium phage Saguaro

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KAG8|A0A386KAG8_9CAUD Exonuclease OS=Mycobacterium phage Saguaro OX=2315616 GN=53 PE=4 SV=1
MM1 pKa = 7.59AVTTVLFDD9 pKa = 3.51TAAQAGSKK17 pKa = 10.08FDD19 pKa = 3.96PEE21 pKa = 3.94VAAEE25 pKa = 4.28VEE27 pKa = 4.32HH28 pKa = 6.53LAPGLEE34 pKa = 4.15PGEE37 pKa = 4.35VGEE40 pKa = 4.49STLADD45 pKa = 3.63GAVSRR50 pKa = 11.84SKK52 pKa = 10.47IKK54 pKa = 10.37PGAVGPEE61 pKa = 3.63QLAAGGVEE69 pKa = 4.37AANMAANSVPTTALQDD85 pKa = 3.75NSVTAAKK92 pKa = 10.33AGLGVSTAYY101 pKa = 10.33DD102 pKa = 3.16AAGNPVEE109 pKa = 5.21DD110 pKa = 3.27RR111 pKa = 11.84TVYY114 pKa = 9.25LTAAEE119 pKa = 4.37YY120 pKa = 10.76NQLTDD125 pKa = 4.16PDD127 pKa = 4.99PNTTYY132 pKa = 10.9YY133 pKa = 10.19ISS135 pKa = 3.42

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386K9K3|A0A386K9K3_9CAUD Uncharacterized protein OS=Mycobacterium phage Saguaro OX=2315616 GN=89 PE=4 SV=1
MM1 pKa = 7.82PGKK4 pKa = 9.37QVKK7 pKa = 8.26NWKK10 pKa = 9.21QYY12 pKa = 9.1HH13 pKa = 4.96GLRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84GMSKK22 pKa = 10.29ARR24 pKa = 11.84AAAITNAASRR34 pKa = 11.84KK35 pKa = 8.21DD36 pKa = 3.39RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84SRR41 pKa = 11.84SRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 3.33

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

22190

28

2183

238.6

25.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.929 ± 0.397

1.005 ± 0.146

6.169 ± 0.24

5.836 ± 0.231

2.325 ± 0.103

9.297 ± 0.53

2.267 ± 0.188

4.123 ± 0.152

2.388 ± 0.128

8.459 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.109

2.902 ± 0.167

6.372 ± 0.235

3.366 ± 0.198

7.269 ± 0.276

4.903 ± 0.18

6.674 ± 0.159

7.373 ± 0.198

2.01 ± 0.138

2.114 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski