Amino acid dipepetide frequency for Mycobacterium phage Saguaro

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.928AlaAla: 18.928 ± 1.304
1.082AlaCys: 1.082 ± 0.238
8.247AlaAsp: 8.247 ± 0.704
9.149AlaGlu: 9.149 ± 0.736
2.344AlaPhe: 2.344 ± 0.326
10.591AlaGly: 10.591 ± 0.874
2.839AlaHis: 2.839 ± 0.447
4.281AlaIle: 4.281 ± 0.505
3.786AlaLys: 3.786 ± 0.575
11.177AlaLeu: 11.177 ± 1.053
2.704AlaMet: 2.704 ± 0.311
3.065AlaAsn: 3.065 ± 0.35
8.202AlaPro: 8.202 ± 0.615
4.101AlaGln: 4.101 ± 0.538
9.284AlaArg: 9.284 ± 0.643
6.219AlaSer: 6.219 ± 0.497
9.104AlaThr: 9.104 ± 0.716
9.554AlaVal: 9.554 ± 0.633
2.479AlaTrp: 2.479 ± 0.384
2.163AlaTyr: 2.163 ± 0.325
0.0AlaXaa: 0.0 ± 0.0
Cys
1.127CysAla: 1.127 ± 0.267
0.135CysCys: 0.135 ± 0.085
0.721CysAsp: 0.721 ± 0.157
0.586CysGlu: 0.586 ± 0.155
0.135CysPhe: 0.135 ± 0.102
1.397CysGly: 1.397 ± 0.305
0.406CysHis: 0.406 ± 0.123
0.27CysIle: 0.27 ± 0.121
0.045CysLys: 0.045 ± 0.039
0.676CysLeu: 0.676 ± 0.195
0.315CysMet: 0.315 ± 0.134
0.361CysAsn: 0.361 ± 0.117
0.451CysPro: 0.451 ± 0.156
0.315CysGln: 0.315 ± 0.13
1.217CysArg: 1.217 ± 0.319
0.496CysSer: 0.496 ± 0.181
0.406CysThr: 0.406 ± 0.146
0.586CysVal: 0.586 ± 0.177
0.225CysTrp: 0.225 ± 0.115
0.18CysTyr: 0.18 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
7.166AspAla: 7.166 ± 0.609
0.631AspCys: 0.631 ± 0.215
4.011AspAsp: 4.011 ± 0.645
3.605AspGlu: 3.605 ± 0.594
1.577AspPhe: 1.577 ± 0.246
5.994AspGly: 5.994 ± 0.59
1.487AspHis: 1.487 ± 0.344
2.839AspIle: 2.839 ± 0.35
1.532AspLys: 1.532 ± 0.312
4.191AspLeu: 4.191 ± 0.389
1.803AspMet: 1.803 ± 0.277
1.622AspAsn: 1.622 ± 0.218
4.417AspPro: 4.417 ± 0.509
2.073AspGln: 2.073 ± 0.355
4.372AspArg: 4.372 ± 0.46
2.569AspSer: 2.569 ± 0.327
4.777AspThr: 4.777 ± 0.484
3.876AspVal: 3.876 ± 0.345
1.622AspTrp: 1.622 ± 0.254
1.532AspTyr: 1.532 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
8.473GluAla: 8.473 ± 0.624
0.721GluCys: 0.721 ± 0.227
2.524GluAsp: 2.524 ± 0.393
2.208GluGlu: 2.208 ± 0.256
1.487GluPhe: 1.487 ± 0.207
3.786GluGly: 3.786 ± 0.429
1.758GluHis: 1.758 ± 0.246
2.929GluIle: 2.929 ± 0.37
1.127GluLys: 1.127 ± 0.265
6.625GluLeu: 6.625 ± 0.627
1.082GluMet: 1.082 ± 0.303
1.082GluAsn: 1.082 ± 0.211
3.515GluPro: 3.515 ± 0.364
2.884GluGln: 2.884 ± 0.384
4.957GluArg: 4.957 ± 0.559
2.614GluSer: 2.614 ± 0.362
3.2GluThr: 3.2 ± 0.356
5.273GluVal: 5.273 ± 0.566
1.037GluTrp: 1.037 ± 0.218
1.082GluTyr: 1.082 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
2.749PheAla: 2.749 ± 0.342
0.18PheCys: 0.18 ± 0.082
2.344PheAsp: 2.344 ± 0.333
1.037PheGlu: 1.037 ± 0.196
0.406PhePhe: 0.406 ± 0.125
2.569PheGly: 2.569 ± 0.482
0.856PheHis: 0.856 ± 0.261
0.991PheIle: 0.991 ± 0.221
0.766PheLys: 0.766 ± 0.181
1.803PheLeu: 1.803 ± 0.259
0.406PheMet: 0.406 ± 0.146
0.901PheAsn: 0.901 ± 0.171
1.037PhePro: 1.037 ± 0.198
0.586PheGln: 0.586 ± 0.14
1.713PheArg: 1.713 ± 0.275
1.307PheSer: 1.307 ± 0.225
1.442PheThr: 1.442 ± 0.296
1.397PheVal: 1.397 ± 0.256
0.406PheTrp: 0.406 ± 0.138
0.361PheTyr: 0.361 ± 0.108
0.0PheXaa: 0.0 ± 0.0
Gly
8.608GlyAla: 8.608 ± 1.011
0.946GlyCys: 0.946 ± 0.214
5.408GlyAsp: 5.408 ± 0.554
4.777GlyGlu: 4.777 ± 0.424
1.938GlyPhe: 1.938 ± 0.336
10.861GlyGly: 10.861 ± 1.885
1.803GlyHis: 1.803 ± 0.365
4.191GlyIle: 4.191 ± 0.477
3.02GlyLys: 3.02 ± 0.392
8.292GlyLeu: 8.292 ± 0.809
1.667GlyMet: 1.667 ± 0.269
2.794GlyAsn: 2.794 ± 0.424
4.507GlyPro: 4.507 ± 0.556
3.605GlyGln: 3.605 ± 0.39
6.94GlyArg: 6.94 ± 0.524
3.966GlySer: 3.966 ± 0.538
6.58GlyThr: 6.58 ± 0.796
7.571GlyVal: 7.571 ± 0.731
2.344GlyTrp: 2.344 ± 0.456
3.155GlyTyr: 3.155 ± 0.411
0.0GlyXaa: 0.0 ± 0.0
His
2.118HisAla: 2.118 ± 0.281
0.225HisCys: 0.225 ± 0.114
1.397HisAsp: 1.397 ± 0.248
1.352HisGlu: 1.352 ± 0.252
0.496HisPhe: 0.496 ± 0.124
1.938HisGly: 1.938 ± 0.386
1.037HisHis: 1.037 ± 0.245
1.487HisIle: 1.487 ± 0.247
0.451HisLys: 0.451 ± 0.176
1.803HisLeu: 1.803 ± 0.397
0.451HisMet: 0.451 ± 0.126
1.082HisAsn: 1.082 ± 0.304
1.938HisPro: 1.938 ± 0.335
0.721HisGln: 0.721 ± 0.192
1.487HisArg: 1.487 ± 0.253
0.811HisSer: 0.811 ± 0.18
1.577HisThr: 1.577 ± 0.291
0.991HisVal: 0.991 ± 0.23
0.451HisTrp: 0.451 ± 0.124
0.856HisTyr: 0.856 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
5.453IleAla: 5.453 ± 0.465
0.315IleCys: 0.315 ± 0.157
4.236IleAsp: 4.236 ± 0.413
3.696IleGlu: 3.696 ± 0.413
0.766IlePhe: 0.766 ± 0.147
4.462IleGly: 4.462 ± 0.492
0.631IleHis: 0.631 ± 0.146
1.532IleIle: 1.532 ± 0.33
0.856IleLys: 0.856 ± 0.249
1.758IleLeu: 1.758 ± 0.229
0.721IleMet: 0.721 ± 0.21
1.803IleAsn: 1.803 ± 0.258
2.569IlePro: 2.569 ± 0.287
1.172IleGln: 1.172 ± 0.236
2.524IleArg: 2.524 ± 0.43
1.938IleSer: 1.938 ± 0.282
2.659IleThr: 2.659 ± 0.269
3.155IleVal: 3.155 ± 0.462
0.315IleTrp: 0.315 ± 0.109
0.676IleTyr: 0.676 ± 0.165
0.0IleXaa: 0.0 ± 0.0
Lys
2.839LysAla: 2.839 ± 0.324
0.135LysCys: 0.135 ± 0.074
1.307LysAsp: 1.307 ± 0.245
1.217LysGlu: 1.217 ± 0.196
0.315LysPhe: 0.315 ± 0.132
2.298LysGly: 2.298 ± 0.376
0.451LysHis: 0.451 ± 0.167
1.082LysIle: 1.082 ± 0.2
0.766LysLys: 0.766 ± 0.194
2.434LysLeu: 2.434 ± 0.358
0.586LysMet: 0.586 ± 0.163
0.901LysAsn: 0.901 ± 0.201
0.991LysPro: 0.991 ± 0.206
0.766LysGln: 0.766 ± 0.183
1.487LysArg: 1.487 ± 0.266
1.397LysSer: 1.397 ± 0.268
1.532LysThr: 1.532 ± 0.214
2.344LysVal: 2.344 ± 0.31
0.406LysTrp: 0.406 ± 0.124
0.631LysTyr: 0.631 ± 0.17
0.0LysXaa: 0.0 ± 0.0
Leu
13.836LeuAla: 13.836 ± 0.921
0.856LeuCys: 0.856 ± 0.219
5.633LeuAsp: 5.633 ± 0.561
4.642LeuGlu: 4.642 ± 0.341
1.758LeuPhe: 1.758 ± 0.27
7.256LeuGly: 7.256 ± 0.71
1.758LeuHis: 1.758 ± 0.332
3.56LeuIle: 3.56 ± 0.406
1.758LeuLys: 1.758 ± 0.346
6.985LeuLeu: 6.985 ± 0.594
1.667LeuMet: 1.667 ± 0.276
2.389LeuAsn: 2.389 ± 0.381
6.039LeuPro: 6.039 ± 0.564
1.893LeuGln: 1.893 ± 0.281
5.363LeuArg: 5.363 ± 0.456
4.867LeuSer: 4.867 ± 0.529
5.318LeuThr: 5.318 ± 0.612
6.129LeuVal: 6.129 ± 0.568
1.082LeuTrp: 1.082 ± 0.237
1.803LeuTyr: 1.803 ± 0.271
0.0LeuXaa: 0.0 ± 0.0
Met
3.335MetAla: 3.335 ± 0.39
0.18MetCys: 0.18 ± 0.096
0.811MetAsp: 0.811 ± 0.179
1.037MetGlu: 1.037 ± 0.211
0.631MetPhe: 0.631 ± 0.193
1.893MetGly: 1.893 ± 0.341
0.361MetHis: 0.361 ± 0.128
0.676MetIle: 0.676 ± 0.176
0.496MetLys: 0.496 ± 0.163
1.667MetLeu: 1.667 ± 0.218
0.18MetMet: 0.18 ± 0.088
0.541MetAsn: 0.541 ± 0.144
2.344MetPro: 2.344 ± 0.302
0.27MetGln: 0.27 ± 0.101
1.442MetArg: 1.442 ± 0.242
1.938MetSer: 1.938 ± 0.274
2.298MetThr: 2.298 ± 0.358
1.442MetVal: 1.442 ± 0.228
0.225MetTrp: 0.225 ± 0.125
0.406MetTyr: 0.406 ± 0.095
0.0MetXaa: 0.0 ± 0.0
Asn
3.966AsnAla: 3.966 ± 0.447
0.406AsnCys: 0.406 ± 0.115
1.893AsnAsp: 1.893 ± 0.333
1.758AsnGlu: 1.758 ± 0.288
0.406AsnPhe: 0.406 ± 0.179
2.073AsnGly: 2.073 ± 0.388
0.901AsnHis: 0.901 ± 0.219
1.442AsnIle: 1.442 ± 0.269
0.676AsnLys: 0.676 ± 0.162
2.163AsnLeu: 2.163 ± 0.285
0.676AsnMet: 0.676 ± 0.212
0.676AsnAsn: 0.676 ± 0.216
2.073AsnPro: 2.073 ± 0.274
1.082AsnGln: 1.082 ± 0.28
1.577AsnArg: 1.577 ± 0.268
1.487AsnSer: 1.487 ± 0.244
2.163AsnThr: 2.163 ± 0.302
2.298AsnVal: 2.298 ± 0.36
0.766AsnTrp: 0.766 ± 0.203
0.541AsnTyr: 0.541 ± 0.135
0.0AsnXaa: 0.0 ± 0.0
Pro
7.121ProAla: 7.121 ± 0.712
0.586ProCys: 0.586 ± 0.217
5.183ProAsp: 5.183 ± 0.541
4.417ProGlu: 4.417 ± 0.395
1.803ProPhe: 1.803 ± 0.26
7.932ProGly: 7.932 ± 0.768
1.442ProHis: 1.442 ± 0.346
2.208ProIle: 2.208 ± 0.295
1.127ProLys: 1.127 ± 0.215
4.011ProLeu: 4.011 ± 0.397
1.262ProMet: 1.262 ± 0.233
1.713ProAsn: 1.713 ± 0.285
5.228ProPro: 5.228 ± 0.536
1.713ProGln: 1.713 ± 0.247
3.56ProArg: 3.56 ± 0.446
3.155ProSer: 3.155 ± 0.4
4.822ProThr: 4.822 ± 0.518
4.777ProVal: 4.777 ± 0.527
0.901ProTrp: 0.901 ± 0.194
0.721ProTyr: 0.721 ± 0.194
0.0ProXaa: 0.0 ± 0.0
Gln
4.912GlnAla: 4.912 ± 0.645
0.18GlnCys: 0.18 ± 0.091
1.082GlnAsp: 1.082 ± 0.189
1.352GlnGlu: 1.352 ± 0.229
1.082GlnPhe: 1.082 ± 0.232
2.659GlnGly: 2.659 ± 0.294
0.361GlnHis: 0.361 ± 0.125
1.532GlnIle: 1.532 ± 0.371
0.406GlnLys: 0.406 ± 0.109
4.732GlnLeu: 4.732 ± 0.394
1.172GlnMet: 1.172 ± 0.263
0.856GlnAsn: 0.856 ± 0.227
2.163GlnPro: 2.163 ± 0.312
1.352GlnGln: 1.352 ± 0.314
2.974GlnArg: 2.974 ± 0.351
1.487GlnSer: 1.487 ± 0.288
1.307GlnThr: 1.307 ± 0.195
2.479GlnVal: 2.479 ± 0.327
0.946GlnTrp: 0.946 ± 0.255
0.631GlnTyr: 0.631 ± 0.15
0.0GlnXaa: 0.0 ± 0.0
Arg
9.374ArgAla: 9.374 ± 0.699
0.766ArgCys: 0.766 ± 0.195
3.966ArgAsp: 3.966 ± 0.456
4.597ArgGlu: 4.597 ± 0.558
1.758ArgPhe: 1.758 ± 0.221
4.822ArgGly: 4.822 ± 0.498
1.758ArgHis: 1.758 ± 0.273
2.929ArgIle: 2.929 ± 0.335
1.848ArgLys: 1.848 ± 0.325
6.4ArgLeu: 6.4 ± 0.537
1.758ArgMet: 1.758 ± 0.285
2.479ArgAsn: 2.479 ± 0.308
3.876ArgPro: 3.876 ± 0.441
3.11ArgGln: 3.11 ± 0.415
7.256ArgArg: 7.256 ± 0.879
3.47ArgSer: 3.47 ± 0.524
4.552ArgThr: 4.552 ± 0.422
3.966ArgVal: 3.966 ± 0.42
1.893ArgTrp: 1.893 ± 0.282
2.118ArgTyr: 2.118 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
5.633SerAla: 5.633 ± 0.598
0.766SerCys: 0.766 ± 0.25
2.524SerAsp: 2.524 ± 0.386
2.704SerGlu: 2.704 ± 0.351
1.217SerPhe: 1.217 ± 0.214
6.039SerGly: 6.039 ± 0.701
0.901SerHis: 0.901 ± 0.213
1.487SerIle: 1.487 ± 0.276
1.487SerLys: 1.487 ± 0.314
4.507SerLeu: 4.507 ± 0.479
0.991SerMet: 0.991 ± 0.222
1.442SerAsn: 1.442 ± 0.286
2.839SerPro: 2.839 ± 0.323
1.848SerGln: 1.848 ± 0.28
3.921SerArg: 3.921 ± 0.507
2.163SerSer: 2.163 ± 0.325
3.245SerThr: 3.245 ± 0.392
3.02SerVal: 3.02 ± 0.372
1.352SerTrp: 1.352 ± 0.236
0.946SerTyr: 0.946 ± 0.244
0.0SerXaa: 0.0 ± 0.0
Thr
9.509ThrAla: 9.509 ± 0.686
0.451ThrCys: 0.451 ± 0.146
3.921ThrAsp: 3.921 ± 0.452
3.515ThrGlu: 3.515 ± 0.402
1.848ThrPhe: 1.848 ± 0.304
6.49ThrGly: 6.49 ± 0.657
1.262ThrHis: 1.262 ± 0.298
3.11ThrIle: 3.11 ± 0.323
1.487ThrLys: 1.487 ± 0.301
4.236ThrLeu: 4.236 ± 0.379
2.208ThrMet: 2.208 ± 0.307
1.442ThrAsn: 1.442 ± 0.262
4.867ThrPro: 4.867 ± 0.581
2.208ThrGln: 2.208 ± 0.271
3.786ThrArg: 3.786 ± 0.323
3.921ThrSer: 3.921 ± 0.436
4.417ThrThr: 4.417 ± 0.569
5.724ThrVal: 5.724 ± 0.577
0.991ThrTrp: 0.991 ± 0.222
1.352ThrTyr: 1.352 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
9.374ValAla: 9.374 ± 0.809
0.676ValCys: 0.676 ± 0.182
4.011ValAsp: 4.011 ± 0.401
4.597ValGlu: 4.597 ± 0.362
1.803ValPhe: 1.803 ± 0.249
6.985ValGly: 6.985 ± 0.698
1.352ValHis: 1.352 ± 0.232
3.29ValIle: 3.29 ± 0.43
1.532ValLys: 1.532 ± 0.244
7.481ValLeu: 7.481 ± 0.579
1.667ValMet: 1.667 ± 0.264
2.479ValAsn: 2.479 ± 0.333
4.462ValPro: 4.462 ± 0.407
2.298ValGln: 2.298 ± 0.288
5.633ValArg: 5.633 ± 0.627
3.11ValSer: 3.11 ± 0.395
4.687ValThr: 4.687 ± 0.674
5.363ValVal: 5.363 ± 0.529
1.758ValTrp: 1.758 ± 0.258
1.172ValTyr: 1.172 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
2.389TrpAla: 2.389 ± 0.361
0.451TrpCys: 0.451 ± 0.16
1.127TrpAsp: 1.127 ± 0.218
0.676TrpGlu: 0.676 ± 0.177
0.811TrpPhe: 0.811 ± 0.232
1.127TrpGly: 1.127 ± 0.255
0.811TrpHis: 0.811 ± 0.181
0.766TrpIle: 0.766 ± 0.156
0.406TrpLys: 0.406 ± 0.141
1.848TrpLeu: 1.848 ± 0.228
0.451TrpMet: 0.451 ± 0.148
0.721TrpAsn: 0.721 ± 0.183
0.991TrpPro: 0.991 ± 0.227
0.766TrpGln: 0.766 ± 0.201
1.532TrpArg: 1.532 ± 0.219
1.172TrpSer: 1.172 ± 0.221
1.307TrpThr: 1.307 ± 0.314
1.938TrpVal: 1.938 ± 0.287
0.496TrpTrp: 0.496 ± 0.141
0.315TrpTyr: 0.315 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.389TyrAla: 2.389 ± 0.386
0.361TyrCys: 0.361 ± 0.146
1.352TyrAsp: 1.352 ± 0.226
1.442TyrGlu: 1.442 ± 0.244
0.676TyrPhe: 0.676 ± 0.167
1.803TyrGly: 1.803 ± 0.313
0.496TyrHis: 0.496 ± 0.173
0.631TyrIle: 0.631 ± 0.217
0.315TyrLys: 0.315 ± 0.107
1.803TyrLeu: 1.803 ± 0.278
0.361TyrMet: 0.361 ± 0.116
0.676TyrAsn: 0.676 ± 0.157
1.217TyrPro: 1.217 ± 0.236
0.901TyrGln: 0.901 ± 0.196
1.667TyrArg: 1.667 ± 0.348
0.946TyrSer: 0.946 ± 0.177
1.352TyrThr: 1.352 ± 0.276
1.848TyrVal: 1.848 ± 0.246
0.406TyrTrp: 0.406 ± 0.126
0.496TyrTyr: 0.496 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (22190 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski