Paenisporosarcina antarctica
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P7A3S5|A0A4P7A3S5_9BACL Uncharacterized protein OS=Paenisporosarcina antarctica OX=417367 GN=E2636_16840 PE=4 SV=1
MM1 pKa = 7.29 FSGYY5 pKa = 10.94 KK6 pKa = 10.18 LIEE9 pKa = 3.92 VDD11 pKa = 4.58 GGDD14 pKa = 3.09 LSGYY18 pKa = 10.03 RR19 pKa = 11.84 EE20 pKa = 4.23 TNVVVDD26 pKa = 3.14 IGYY29 pKa = 10.81 GDD31 pKa = 3.54 RR32 pKa = 11.84 EE33 pKa = 3.97 YY34 pKa = 10.38 WAFTNEE40 pKa = 3.58 YY41 pKa = 8.43 GQLVRR46 pKa = 11.84 VIADD50 pKa = 3.95 EE51 pKa = 5.84 IIIQDD56 pKa = 3.84 DD57 pKa = 3.73 SNEE60 pKa = 4.0 PVLSSGRR67 pKa = 11.84 YY68 pKa = 8.18 YY69 pKa = 10.94 SDD71 pKa = 3.3 EE72 pKa = 4.16 AKK74 pKa = 10.96 VPGVEE79 pKa = 4.89 SDD81 pKa = 3.87 VLDD84 pKa = 4.29 EE85 pKa = 4.17 GHH87 pKa = 6.92 IVADD91 pKa = 3.9 SNGGVSNAYY100 pKa = 10.46 NITPQNSTLNRR111 pKa = 11.84 HH112 pKa = 5.47 GDD114 pKa = 3.32 QAYY117 pKa = 8.24 MEE119 pKa = 4.23 NVIRR123 pKa = 11.84 SAGGATNFEE132 pKa = 4.75 AIITYY137 pKa = 9.8 PNTEE141 pKa = 3.94 TQIPSSYY148 pKa = 10.51 QYY150 pKa = 10.89 TYY152 pKa = 10.69 TLMGNEE158 pKa = 4.15 IVDD161 pKa = 3.54 TFDD164 pKa = 3.41 NVNPDD169 pKa = 3.51 EE170 pKa = 4.65 VNTSLGLTEE179 pKa = 5.2 SEE181 pKa = 4.72 PSDD184 pKa = 3.45 STSSNTNGDD193 pKa = 3.16 ISSVDD198 pKa = 3.32 TNGNGKK204 pKa = 7.9 VTIKK208 pKa = 9.78 EE209 pKa = 4.01 AKK211 pKa = 9.84 DD212 pKa = 3.22 AGFSMPITSDD222 pKa = 2.65 HH223 pKa = 6.27 WLYY226 pKa = 11.35 AYY228 pKa = 8.87 MQDD231 pKa = 3.47 NDD233 pKa = 3.77 GDD235 pKa = 4.21 GMVGEE240 pKa = 4.67
Molecular weight: 26.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.592
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A4P7A318|A0A4P7A318_9BACL SRPBCC family protein OS=Paenisporosarcina antarctica OX=417367 GN=E2636_17745 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPNKK9 pKa = 8.16 RR10 pKa = 11.84 KK11 pKa = 9.54 HH12 pKa = 5.99 SKK14 pKa = 8.91 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 9.98 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3570
0
3570
1051335
30
1549
294.5
33.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.901 ± 0.044
0.671 ± 0.012
5.098 ± 0.032
7.224 ± 0.045
4.648 ± 0.034
6.77 ± 0.037
2.12 ± 0.02
8.165 ± 0.041
6.597 ± 0.03
9.853 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.889 ± 0.022
4.329 ± 0.035
3.544 ± 0.026
3.792 ± 0.029
3.941 ± 0.028
6.281 ± 0.028
5.643 ± 0.031
7.182 ± 0.032
1.021 ± 0.014
3.33 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here