Streptococcus satellite phage Javan571
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRJ3|A0A4D5ZRJ3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan571 OX=2558753 GN=JavanS571_0002 PE=4 SV=1
MM1 pKa = 7.41 NSFVALDD8 pKa = 3.65 VEE10 pKa = 4.78 TANSFRR16 pKa = 11.84 GSLCAIGLVKK26 pKa = 10.5 FEE28 pKa = 4.67 NGKK31 pKa = 8.54 ITDD34 pKa = 4.33 FFYY37 pKa = 11.22 SHH39 pKa = 7.32 INPEE43 pKa = 3.84 EE44 pKa = 3.81 EE45 pKa = 3.71 FQYY48 pKa = 11.09 FF49 pKa = 3.31
Molecular weight: 5.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.485
IPC2_protein 4.673
IPC_protein 4.393
Toseland 4.266
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.202
Rodwell 4.24
Grimsley 4.19
Solomon 4.317
Lehninger 4.266
Nozaki 4.457
DTASelect 4.533
Thurlkill 4.279
EMBOSS 4.215
Sillero 4.507
Patrickios 3.35
IPC_peptide 4.329
IPC2_peptide 4.495
IPC2.peptide.svr19 4.451
Protein with the highest isoelectric point:
>tr|A0A4D5ZVP4|A0A4D5ZVP4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan571 OX=2558753 GN=JavanS571_0004 PE=4 SV=1
MM1 pKa = 7.61 TNFQTYY7 pKa = 10.09 LGYY10 pKa = 10.01 GQPYY14 pKa = 10.03 RR15 pKa = 11.84 MADD18 pKa = 3.07 EE19 pKa = 5.76 HH20 pKa = 5.9 IWKK23 pKa = 9.84 DD24 pKa = 3.46 RR25 pKa = 11.84 RR26 pKa = 11.84 PTLEE30 pKa = 4.79 HH31 pKa = 6.51 IKK33 pKa = 9.99 EE34 pKa = 3.96 QQRR37 pKa = 11.84 LKK39 pKa = 10.73 KK40 pKa = 10.24 LKK42 pKa = 10.01 KK43 pKa = 9.67 KK44 pKa = 9.88 RR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 9.34
Molecular weight: 5.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.809
IPC_protein 10.175
Toseland 10.672
ProMoST 10.292
Dawson 10.774
Bjellqvist 10.394
Wikipedia 10.921
Rodwell 11.272
Grimsley 10.818
Solomon 10.818
Lehninger 10.804
Nozaki 10.628
DTASelect 10.394
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 11.023
IPC_peptide 10.833
IPC2_peptide 8.96
IPC2.peptide.svr19 8.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2568
39
533
151.1
17.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.269 ± 0.462
0.467 ± 0.148
5.763 ± 0.386
8.178 ± 0.93
4.011 ± 0.458
4.79 ± 0.571
1.908 ± 0.251
6.114 ± 0.603
9.54 ± 0.604
10.436 ± 0.773
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.327
4.868 ± 0.651
3.388 ± 0.585
4.984 ± 0.382
5.763 ± 0.458
4.829 ± 0.5
5.919 ± 0.521
5.101 ± 0.504
0.779 ± 0.118
4.673 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here