Bifidobacterium choloepi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1599 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5MYS2|A0A6I5MYS2_9BIFI Esterase family protein OS=Bifidobacterium choloepi OX=2614131 GN=F6S87_03990 PE=4 SV=1
MM1 pKa = 7.59GRR3 pKa = 11.84LTKK6 pKa = 10.74LCTAVAASLALAGVAVGPAFAGEE29 pKa = 4.22SSGGSSTTTSTIDD42 pKa = 3.39AGNGTITVDD51 pKa = 2.83YY52 pKa = 9.48HH53 pKa = 4.29YY54 pKa = 9.24TYY56 pKa = 10.24TSSAGEE62 pKa = 3.8QTQFPVKK69 pKa = 9.75GANVMLYY76 pKa = 10.22KK77 pKa = 10.63AAGWNDD83 pKa = 3.28SGDD86 pKa = 3.7LVLTGDD92 pKa = 4.81FAALCEE98 pKa = 4.3GASLDD103 pKa = 4.17CSAITDD109 pKa = 3.73TGRR112 pKa = 11.84LADD115 pKa = 4.4NDD117 pKa = 4.17TQRR120 pKa = 11.84DD121 pKa = 3.86LATSLLTALATSDD134 pKa = 3.98IAFDD138 pKa = 3.69TQDD141 pKa = 3.21AVIVSDD147 pKa = 3.69GTARR151 pKa = 11.84YY152 pKa = 9.79VDD154 pKa = 3.95ADD156 pKa = 3.88GEE158 pKa = 4.4ATDD161 pKa = 5.53DD162 pKa = 3.95VAFTGLGDD170 pKa = 3.63ALYY173 pKa = 9.76FIRR176 pKa = 11.84VGGQILDD183 pKa = 3.58ADD185 pKa = 4.44AYY187 pKa = 10.12SHH189 pKa = 7.0LMYY192 pKa = 10.84CNEE195 pKa = 3.68TAMFIDD201 pKa = 4.71LKK203 pKa = 10.94SAVSGDD209 pKa = 3.82DD210 pKa = 3.26VAGTVSRR217 pKa = 11.84TVSLEE222 pKa = 3.78PKK224 pKa = 10.07ASCTLGPDD232 pKa = 4.5PIHH235 pKa = 5.7VTKK238 pKa = 10.72VWDD241 pKa = 4.97DD242 pKa = 3.59GDD244 pKa = 3.9STARR248 pKa = 11.84PSSVDD253 pKa = 2.91VTLYY257 pKa = 10.94GRR259 pKa = 11.84YY260 pKa = 7.97TDD262 pKa = 3.56IEE264 pKa = 4.3GNEE267 pKa = 3.99VVEE270 pKa = 4.75TIEE273 pKa = 4.21TQTLSADD280 pKa = 3.75NDD282 pKa = 3.45WTYY285 pKa = 11.35VWSGLPDD292 pKa = 3.92DD293 pKa = 4.28YY294 pKa = 11.79YY295 pKa = 11.01EE296 pKa = 4.45YY297 pKa = 10.63FVQEE301 pKa = 3.62VDD303 pKa = 3.63VPDD306 pKa = 5.31GYY308 pKa = 9.35TVSYY312 pKa = 10.81SRR314 pKa = 11.84TDD316 pKa = 3.22VTEE319 pKa = 5.13EE320 pKa = 3.94SATWTVTNTSEE331 pKa = 4.89SEE333 pKa = 4.03SSTPGDD339 pKa = 3.51SEE341 pKa = 4.77TPGTSDD347 pKa = 4.25SDD349 pKa = 4.26DD350 pKa = 3.35GTTPGTSGSTGSTSASASATATSTTPAQTGANIALVALVAVSALMLGVLLIVEE403 pKa = 4.69IRR405 pKa = 11.84RR406 pKa = 11.84ASKK409 pKa = 10.42KK410 pKa = 10.41AA411 pKa = 3.17

Molecular weight: 42.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5NMU5|A0A6I5NMU5_9BIFI DNA helicase OS=Bifidobacterium choloepi OX=2614131 GN=F6S87_05435 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1599

0

1599

615902

29

2363

385.2

41.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.338 ± 0.078

0.912 ± 0.016

7.181 ± 0.052

5.793 ± 0.051

3.483 ± 0.036

8.256 ± 0.05

2.055 ± 0.03

5.008 ± 0.046

3.526 ± 0.055

8.717 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.026

3.19 ± 0.04

4.609 ± 0.044

3.055 ± 0.031

5.968 ± 0.07

5.97 ± 0.063

6.244 ± 0.071

8.075 ± 0.055

1.335 ± 0.026

2.715 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski