fungal sp. No.14919
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14332 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V1TMA7|A0A1V1TMA7_9FUNG Uncharacterized protein OS=fungal sp. No.14919 OX=1813822 GN=ANO14919_114450 PE=4 SV=1
MM1 pKa = 7.22 SLSMSPLWLSVDD13 pKa = 3.51 YY14 pKa = 11.29 CDD16 pKa = 4.64 INVTITTQADD26 pKa = 3.6 IEE28 pKa = 4.58 SLHH31 pKa = 5.12 ATPYY35 pKa = 9.66 EE36 pKa = 4.06 FCFNNVTVANAEE48 pKa = 4.08 GTLTFDD54 pKa = 5.06 NFTQVNGLYY63 pKa = 10.45 VQDD66 pKa = 4.23 SPNLEE71 pKa = 3.89 ALSFPDD77 pKa = 4.62 LGRR80 pKa = 11.84 LSALGVYY87 pKa = 9.81 DD88 pKa = 5.63 ALALSNISLPEE99 pKa = 3.87 IQSVGLLPFGGGGWSVPPPWPGAEE123 pKa = 3.92 DD124 pKa = 3.49 RR125 pKa = 11.84 SLIGVTINNAPALKK139 pKa = 9.79 TIDD142 pKa = 3.9 FDD144 pKa = 4.11 FLSGFFTLEE153 pKa = 3.72 LVGADD158 pKa = 3.49 GLTDD162 pKa = 4.38 FGPTGYY168 pKa = 10.62 DD169 pKa = 2.86 EE170 pKa = 5.99 GILRR174 pKa = 11.84 RR175 pKa = 11.84 INSSDD180 pKa = 3.27 TLSVDD185 pKa = 3.36 GCFNLANVQFARR197 pKa = 11.84 FVQITGRR204 pKa = 11.84 TDD206 pKa = 3.17 CEE208 pKa = 3.9 YY209 pKa = 11.73 LMLNYY214 pKa = 9.99 HH215 pKa = 5.83 SAYY218 pKa = 10.67 NLTLIDD224 pKa = 3.85 TAHH227 pKa = 6.3 SRR229 pKa = 11.84 LDD231 pKa = 3.17 ILAPFAVNGTLVADD245 pKa = 4.35 SLQLSPEE252 pKa = 4.09 NSSSYY257 pKa = 11.01 DD258 pKa = 3.44 PQPLYY263 pKa = 10.69 FISSIGEE270 pKa = 4.03 DD271 pKa = 3.04 AHH273 pKa = 7.01 LLSGANVDD281 pKa = 3.91 LSLDD285 pKa = 3.64 EE286 pKa = 4.78 VEE288 pKa = 4.55 TLGGSLVASNNKK300 pKa = 9.25 NCTFSFDD307 pKa = 3.56 KK308 pKa = 10.96 LSEE311 pKa = 4.25 VKK313 pKa = 10.86 GNISMTDD320 pKa = 3.42 NPDD323 pKa = 3.23 SALPWFPDD331 pKa = 3.14 LRR333 pKa = 11.84 RR334 pKa = 11.84 AANIEE339 pKa = 3.62 LRR341 pKa = 11.84 GNINTSHH348 pKa = 6.64 GPNIFPALTTVPGTVTIEE366 pKa = 3.62 AWNSDD371 pKa = 4.19 FNCSQLVKK379 pKa = 10.6 QMQAGIIQNLVCNGTDD395 pKa = 3.3 GTQGDD400 pKa = 4.32 TSGSPSSPAGGAWTVLGIAAGIMVLQNTIPVWW432 pKa = 3.47
Molecular weight: 46.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.91
IPC_protein 3.935
Toseland 3.706
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.291
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.05
Patrickios 1.291
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A1V1T950|A0A1V1T950_9FUNG Uncharacterized protein OS=fungal sp. No.14919 OX=1813822 GN=ANO14919_068420 PE=4 SV=1
MM1 pKa = 6.57 VTGPVPILGLPPGPPLPGLPLGTCASIGITTGVRR35 pKa = 11.84 IATSVGITTGVGITTGVGIATSIGITTTSIRR66 pKa = 11.84 IAASVGITTSVRR78 pKa = 11.84 ITTSVGITTGVGIATSIGITTGVGIATSIGITTTSIRR115 pKa = 11.84 IAASVGITTSVRR127 pKa = 11.84 ITTSVGITTGVGIATSIGITTGVGIATSIGITTTSIRR164 pKa = 11.84 IAASVGITTTSIRR177 pKa = 11.84 IAASVGITTSVRR189 pKa = 11.84 ITTSVGITTGVGIATSIGITTGVGIATSIGITTTSIRR226 pKa = 11.84 IAASVGITTGVRR238 pKa = 11.84 TAAGVGIATSVGITAGVGITTSVGIATSIGTATRR272 pKa = 11.84 TPTARR277 pKa = 11.84 ATVRR281 pKa = 3.27
Molecular weight: 26.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.574
Toseland 12.735
ProMoST 13.247
Dawson 12.735
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.237
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.735
DTASelect 12.735
Thurlkill 12.735
EMBOSS 13.247
Sillero 12.735
Patrickios 11.974
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14332
0
14332
6368111
29
6731
444.3
49.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.646 ± 0.018
1.227 ± 0.007
5.788 ± 0.015
6.059 ± 0.016
3.705 ± 0.013
6.974 ± 0.017
2.401 ± 0.01
5.027 ± 0.016
4.579 ± 0.018
8.953 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.032 ± 0.008
3.72 ± 0.011
6.034 ± 0.025
3.883 ± 0.014
6.234 ± 0.019
8.188 ± 0.023
6.052 ± 0.018
6.153 ± 0.015
1.511 ± 0.007
2.835 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here