Rhodobacteraceae bacterium WDS4C29

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Salibaculum

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1P607|A0A2V1P607_9RHOB Diaminobutyrate--2-oxoglutarate transaminase OS=Rhodobacteraceae bacterium WDS4C29 OX=1914409 GN=ectB PE=3 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84YY3 pKa = 8.64PVTIAIVGLAALSGCLDD20 pKa = 3.43GTNPFMVDD28 pKa = 3.2SPDD31 pKa = 3.6GTGSEE36 pKa = 4.28QLDD39 pKa = 3.81EE40 pKa = 4.9NDD42 pKa = 4.3PNTDD46 pKa = 2.74VNNQFLYY53 pKa = 11.0DD54 pKa = 3.99PDD56 pKa = 4.76RR57 pKa = 11.84GLTMNAVQYY66 pKa = 10.73DD67 pKa = 3.78AANDD71 pKa = 3.5EE72 pKa = 4.52LVINNLPFDD81 pKa = 4.52GPDD84 pKa = 3.14GRR86 pKa = 11.84YY87 pKa = 10.69DD88 pKa = 3.96NIAGTTSTDD97 pKa = 3.17ANGITSGVYY106 pKa = 9.59EE107 pKa = 4.33SRR109 pKa = 11.84QTATTGLVKK118 pKa = 10.25HH119 pKa = 5.75YY120 pKa = 10.72AVFVRR125 pKa = 11.84GDD127 pKa = 3.65YY128 pKa = 11.37LEE130 pKa = 4.46ATAANSRR137 pKa = 11.84DD138 pKa = 3.2WFGFGYY144 pKa = 10.63GGANINRR151 pKa = 11.84SDD153 pKa = 3.58FGLPSGIGEE162 pKa = 3.97YY163 pKa = 10.36VYY165 pKa = 10.65RR166 pKa = 11.84GVYY169 pKa = 9.94GGARR173 pKa = 11.84TFDD176 pKa = 3.24NGGGIEE182 pKa = 4.33LTAGDD187 pKa = 4.08ATLILDD193 pKa = 4.36VLDD196 pKa = 4.66FDD198 pKa = 6.21PII200 pKa = 3.69

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1P7Y8|A0A2V1P7Y8_9RHOB ATP synthase subunit c OS=Rhodobacteraceae bacterium WDS4C29 OX=1914409 GN=atpE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3136

0

3136

979202

21

14339

312.2

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.154 ± 0.061

0.88 ± 0.016

6.532 ± 0.069

6.191 ± 0.044

3.649 ± 0.036

8.798 ± 0.042

2.069 ± 0.025

5.02 ± 0.032

3.052 ± 0.044

9.897 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.031

2.541 ± 0.046

5.138 ± 0.036

3.165 ± 0.026

6.849 ± 0.066

4.883 ± 0.052

5.526 ± 0.066

7.238 ± 0.035

1.432 ± 0.023

2.225 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski