Amino acid dipepetide frequency for Rhodobacteraceae bacterium WDS4C29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.05AlaAla: 16.05 ± 0.187	1.092AlaCys: 1.092 ± 0.041	7.386AlaAsp: 7.386 ± 0.141	8.551AlaGlu: 8.551 ± 0.113	4.21AlaPhe: 4.21 ± 0.074	10.606AlaGly: 10.606 ± 0.111	2.363AlaHis: 2.363 ± 0.061	5.642AlaIle: 5.642 ± 0.081	3.627AlaLys: 3.627 ± 0.075	13.442AlaLeu: 13.442 ± 0.163	3.872AlaMet: 3.872 ± 0.074	2.723AlaAsn: 2.723 ± 0.067	5.878AlaPro: 5.878 ± 0.102	4.263AlaGln: 4.263 ± 0.071	8.842AlaArg: 8.842 ± 0.136	5.122AlaSer: 5.122 ± 0.106	5.914AlaThr: 5.914 ± 0.08	8.079AlaVal: 8.079 ± 0.099	1.385AlaTrp: 1.385 ± 0.045	2.495AlaTyr: 2.495 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.052CysAla: 1.052 ± 0.04	0.118CysCys: 0.118 ± 0.011	0.655CysAsp: 0.655 ± 0.024	0.469CysGlu: 0.469 ± 0.021	0.324CysPhe: 0.324 ± 0.02	0.999CysGly: 0.999 ± 0.039	0.279CysHis: 0.279 ± 0.02	0.422CysIle: 0.422 ± 0.02	0.205CysLys: 0.205 ± 0.014	0.864CysLeu: 0.864 ± 0.034	0.155CysMet: 0.155 ± 0.013	0.211CysAsn: 0.211 ± 0.015	0.523CysPro: 0.523 ± 0.025	0.227CysGln: 0.227 ± 0.015	0.546CysArg: 0.546 ± 0.024	0.413CysSer: 0.413 ± 0.021	0.451CysThr: 0.451 ± 0.022	0.57CysVal: 0.57 ± 0.026	0.114CysTrp: 0.114 ± 0.01	0.207CysTyr: 0.207 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.46AspAla: 7.46 ± 0.124	0.56AspCys: 0.56 ± 0.024	3.831AspAsp: 3.831 ± 0.122	3.995AspGlu: 3.995 ± 0.073	2.449AspPhe: 2.449 ± 0.046	6.099AspGly: 6.099 ± 0.158	1.512AspHis: 1.512 ± 0.043	3.155AspIle: 3.155 ± 0.061	1.802AspLys: 1.802 ± 0.046	6.841AspLeu: 6.841 ± 0.101	1.881AspMet: 1.881 ± 0.047	1.417AspAsn: 1.417 ± 0.073	4.071AspPro: 4.071 ± 0.069	2.041AspGln: 2.041 ± 0.048	5.018AspArg: 5.018 ± 0.077	2.539AspSer: 2.539 ± 0.102	3.343AspThr: 3.343 ± 0.124	4.262AspVal: 4.262 ± 0.075	1.348AspTrp: 1.348 ± 0.035	1.696AspTyr: 1.696 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	8.695GluAla: 8.695 ± 0.119	0.427GluCys: 0.427 ± 0.023	3.879GluAsp: 3.879 ± 0.07	3.996GluGlu: 3.996 ± 0.102	1.805GluPhe: 1.805 ± 0.047	5.175GluGly: 5.175 ± 0.076	1.163GluHis: 1.163 ± 0.036	3.838GluIle: 3.838 ± 0.068	2.253GluLys: 2.253 ± 0.054	5.277GluLeu: 5.277 ± 0.085	1.846GluMet: 1.846 ± 0.045	1.828GluAsn: 1.828 ± 0.047	2.597GluPro: 2.597 ± 0.066	2.072GluGln: 2.072 ± 0.051	4.213GluArg: 4.213 ± 0.078	2.186GluSer: 2.186 ± 0.052	4.126GluThr: 4.126 ± 0.073	4.668GluVal: 4.668 ± 0.063	0.796GluTrp: 0.796 ± 0.034	1.068GluTyr: 1.068 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.156PheAla: 4.156 ± 0.083	0.441PheCys: 0.441 ± 0.023	2.918PheAsp: 2.918 ± 0.056	2.421PheGlu: 2.421 ± 0.056	1.415PhePhe: 1.415 ± 0.053	3.637PheGly: 3.637 ± 0.073	0.738PheHis: 0.738 ± 0.031	1.637PheIle: 1.637 ± 0.046	0.907PheLys: 0.907 ± 0.032	3.454PheLeu: 3.454 ± 0.079	0.857PheMet: 0.857 ± 0.029	1.045PheAsn: 1.045 ± 0.034	1.479PhePro: 1.479 ± 0.045	0.955PheGln: 0.955 ± 0.032	2.238PheArg: 2.238 ± 0.049	1.978PheSer: 1.978 ± 0.048	2.118PheThr: 2.118 ± 0.056	2.565PheVal: 2.565 ± 0.054	0.607PheTrp: 0.607 ± 0.031	0.921PheTyr: 0.921 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	9.732GlyAla: 9.732 ± 0.111	0.869GlyCys: 0.869 ± 0.036	5.105GlyAsp: 5.105 ± 0.108	4.955GlyGlu: 4.955 ± 0.078	3.657GlyPhe: 3.657 ± 0.063	7.425GlyGly: 7.425 ± 0.108	1.987GlyHis: 1.987 ± 0.046	4.461GlyIle: 4.461 ± 0.076	3.113GlyLys: 3.113 ± 0.068	9.455GlyLeu: 9.455 ± 0.129	2.61GlyMet: 2.61 ± 0.062	2.177GlyAsn: 2.177 ± 0.106	3.976GlyPro: 3.976 ± 0.074	3.336GlyGln: 3.336 ± 0.075	5.867GlyArg: 5.867 ± 0.105	4.127GlySer: 4.127 ± 0.096	4.779GlyThr: 4.779 ± 0.136	6.427GlyVal: 6.427 ± 0.088	1.548GlyTrp: 1.548 ± 0.054	2.372GlyTyr: 2.372 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	2.295HisAla: 2.295 ± 0.054	0.229HisCys: 0.229 ± 0.015	1.437HisAsp: 1.437 ± 0.049	1.147HisGlu: 1.147 ± 0.036	0.816HisPhe: 0.816 ± 0.031	1.978HisGly: 1.978 ± 0.058	0.517HisHis: 0.517 ± 0.025	0.958HisIle: 0.958 ± 0.03	0.525HisLys: 0.525 ± 0.025	2.073HisLeu: 2.073 ± 0.053	0.587HisMet: 0.587 ± 0.025	0.473HisAsn: 0.473 ± 0.024	1.409HisPro: 1.409 ± 0.044	0.563HisGln: 0.563 ± 0.023	1.389HisArg: 1.389 ± 0.042	0.919HisSer: 0.919 ± 0.03	0.791HisThr: 0.791 ± 0.031	1.598HisVal: 1.598 ± 0.041	0.358HisTrp: 0.358 ± 0.02	0.625HisTyr: 0.625 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.694IleAla: 6.694 ± 0.09	0.604IleCys: 0.604 ± 0.026	3.554IleAsp: 3.554 ± 0.07	3.799IleGlu: 3.799 ± 0.065	1.634IlePhe: 1.634 ± 0.051	4.778IleGly: 4.778 ± 0.078	0.909IleHis: 0.909 ± 0.029	2.176IleIle: 2.176 ± 0.053	1.364IleLys: 1.364 ± 0.044	4.57IleLeu: 4.57 ± 0.083	1.057IleMet: 1.057 ± 0.036	1.374IleAsn: 1.374 ± 0.042	2.321IlePro: 2.321 ± 0.055	1.162IleGln: 1.162 ± 0.036	3.229IleArg: 3.229 ± 0.056	2.642IleSer: 2.642 ± 0.06	2.943IleThr: 2.943 ± 0.08	3.615IleVal: 3.615 ± 0.074	0.705IleTrp: 0.705 ± 0.03	1.068IleTyr: 1.068 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.895LysAla: 3.895 ± 0.074	0.179LysCys: 0.179 ± 0.015	1.837LysAsp: 1.837 ± 0.046	1.518LysGlu: 1.518 ± 0.049	0.864LysPhe: 0.864 ± 0.031	2.71LysGly: 2.71 ± 0.071	0.598LysHis: 0.598 ± 0.029	1.461LysIle: 1.461 ± 0.045	1.228LysLys: 1.228 ± 0.054	2.896LysLeu: 2.896 ± 0.06	0.832LysMet: 0.832 ± 0.034	0.725LysAsn: 0.725 ± 0.033	1.736LysPro: 1.736 ± 0.05	0.914LysGln: 0.914 ± 0.031	2.179LysArg: 2.179 ± 0.06	1.677LysSer: 1.677 ± 0.042	1.937LysThr: 1.937 ± 0.048	2.338LysVal: 2.338 ± 0.055	0.388LysTrp: 0.388 ± 0.019	0.611LysTyr: 0.611 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	12.684LeuAla: 12.684 ± 0.149	0.838LeuCys: 0.838 ± 0.031	6.608LeuAsp: 6.608 ± 0.102	5.38LeuGlu: 5.38 ± 0.088	3.598LeuPhe: 3.598 ± 0.073	8.253LeuGly: 8.253 ± 0.101	1.925LeuHis: 1.925 ± 0.051	4.911LeuIle: 4.911 ± 0.088	3.057LeuLys: 3.057 ± 0.065	8.617LeuLeu: 8.617 ± 0.136	2.596LeuMet: 2.596 ± 0.059	2.525LeuAsn: 2.525 ± 0.073	5.351LeuPro: 5.351 ± 0.092	2.794LeuGln: 2.794 ± 0.054	7.129LeuArg: 7.129 ± 0.104	6.502LeuSer: 6.502 ± 0.097	5.964LeuThr: 5.964 ± 0.114	6.919LeuVal: 6.919 ± 0.103	1.327LeuTrp: 1.327 ± 0.042	1.986LeuTyr: 1.986 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	3.64MetAla: 3.64 ± 0.067	0.169MetCys: 0.169 ± 0.013	1.603MetAsp: 1.603 ± 0.045	1.484MetGlu: 1.484 ± 0.046	0.748MetPhe: 0.748 ± 0.03	2.382MetGly: 2.382 ± 0.054	0.418MetHis: 0.418 ± 0.023	1.482MetIle: 1.482 ± 0.046	0.988MetLys: 0.988 ± 0.037	2.49MetLeu: 2.49 ± 0.054	0.739MetMet: 0.739 ± 0.029	0.792MetAsn: 0.792 ± 0.028	1.528MetPro: 1.528 ± 0.038	0.975MetGln: 0.975 ± 0.035	1.967MetArg: 1.967 ± 0.052	1.601MetSer: 1.601 ± 0.04	2.111MetThr: 2.111 ± 0.05	1.924MetVal: 1.924 ± 0.049	0.246MetTrp: 0.246 ± 0.018	0.343MetTyr: 0.343 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.087AsnAla: 3.087 ± 0.067	0.245AsnCys: 0.245 ± 0.018	1.533AsnAsp: 1.533 ± 0.071	1.273AsnGlu: 1.273 ± 0.04	0.91AsnPhe: 0.91 ± 0.03	2.444AsnGly: 2.444 ± 0.091	0.527AsnHis: 0.527 ± 0.024	1.297AsnIle: 1.297 ± 0.039	0.612AsnLys: 0.612 ± 0.026	2.361AsnLeu: 2.361 ± 0.065	0.692AsnMet: 0.692 ± 0.027	0.672AsnAsn: 0.672 ± 0.039	1.837AsnPro: 1.837 ± 0.043	0.731AsnGln: 0.731 ± 0.028	1.792AsnArg: 1.792 ± 0.043	1.108AsnSer: 1.108 ± 0.047	1.425AsnThr: 1.425 ± 0.078	1.822AsnVal: 1.822 ± 0.058	0.434AsnTrp: 0.434 ± 0.019	0.608AsnTyr: 0.608 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.725ProAla: 5.725 ± 0.095	0.379ProCys: 0.379 ± 0.022	4.401ProAsp: 4.401 ± 0.079	4.264ProGlu: 4.264 ± 0.09	1.907ProPhe: 1.907 ± 0.051	4.767ProGly: 4.767 ± 0.081	1.116ProHis: 1.116 ± 0.038	2.213ProIle: 2.213 ± 0.059	1.623ProLys: 1.623 ± 0.046	4.512ProLeu: 4.512 ± 0.086	1.307ProMet: 1.307 ± 0.037	1.33ProAsn: 1.33 ± 0.038	2.293ProPro: 2.293 ± 0.06	1.614ProGln: 1.614 ± 0.047	2.945ProArg: 2.945 ± 0.065	2.327ProSer: 2.327 ± 0.049	2.472ProThr: 2.472 ± 0.068	4.373ProVal: 4.373 ± 0.073	0.672ProTrp: 0.672 ± 0.034	1.14ProTyr: 1.14 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.116GlnAla: 4.116 ± 0.075	0.209GlnCys: 0.209 ± 0.014	1.883GlnAsp: 1.883 ± 0.044	1.689GlnGlu: 1.689 ± 0.053	1.109GlnPhe: 1.109 ± 0.031	2.587GlnGly: 2.587 ± 0.054	0.574GlnHis: 0.574 ± 0.024	1.826GlnIle: 1.826 ± 0.048	1.026GlnLys: 1.026 ± 0.038	2.85GlnLeu: 2.85 ± 0.059	1.074GlnMet: 1.074 ± 0.036	0.843GlnAsn: 0.843 ± 0.03	1.691GlnPro: 1.691 ± 0.048	1.03GlnGln: 1.03 ± 0.042	2.169GlnArg: 2.169 ± 0.045	1.684GlnSer: 1.684 ± 0.05	1.782GlnThr: 1.782 ± 0.049	2.499GlnVal: 2.499 ± 0.053	0.422GlnTrp: 0.422 ± 0.019	0.581GlnTyr: 0.581 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	8.123ArgAla: 8.123 ± 0.109	0.48ArgCys: 0.48 ± 0.024	4.72ArgAsp: 4.72 ± 0.084	3.895ArgGlu: 3.895 ± 0.073	2.695ArgPhe: 2.695 ± 0.062	4.854ArgGly: 4.854 ± 0.081	1.578ArgHis: 1.578 ± 0.044	3.9ArgIle: 3.9 ± 0.076	2.269ArgLys: 2.269 ± 0.059	7.375ArgLeu: 7.375 ± 0.108	1.96ArgMet: 1.96 ± 0.045	1.724ArgAsn: 1.724 ± 0.049	3.511ArgPro: 3.511 ± 0.067	2.378ArgGln: 2.378 ± 0.055	5.224ArgArg: 5.224 ± 0.114	3.291ArgSer: 3.291 ± 0.061	3.104ArgThr: 3.104 ± 0.057	4.798ArgVal: 4.798 ± 0.078	1.025ArgTrp: 1.025 ± 0.037	1.581ArgTyr: 1.581 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.477SerAla: 5.477 ± 0.093	0.451SerCys: 0.451 ± 0.023	3.501SerAsp: 3.501 ± 0.138	2.902SerGlu: 2.902 ± 0.056	2.078SerPhe: 2.078 ± 0.049	5.134SerGly: 5.134 ± 0.114	1.043SerHis: 1.043 ± 0.03	2.277SerIle: 2.277 ± 0.051	1.426SerLys: 1.426 ± 0.039	4.564SerLeu: 4.564 ± 0.08	1.309SerMet: 1.309 ± 0.033	1.315SerAsn: 1.315 ± 0.053	2.472SerPro: 2.472 ± 0.05	1.472SerGln: 1.472 ± 0.038	3.123SerArg: 3.123 ± 0.063	2.309SerSer: 2.309 ± 0.076	2.465SerThr: 2.465 ± 0.068	3.557SerVal: 3.557 ± 0.068	0.705SerTrp: 0.705 ± 0.029	1.248SerTyr: 1.248 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.112ThrAla: 6.112 ± 0.162	0.518ThrCys: 0.518 ± 0.025	3.513ThrAsp: 3.513 ± 0.11	3.307ThrGlu: 3.307 ± 0.061	1.964ThrPhe: 1.964 ± 0.048	5.655ThrGly: 5.655 ± 0.102	1.155ThrHis: 1.155 ± 0.032	2.695ThrIle: 2.695 ± 0.062	1.424ThrLys: 1.424 ± 0.042	6.004ThrLeu: 6.004 ± 0.129	1.258ThrMet: 1.258 ± 0.042	1.303ThrAsn: 1.303 ± 0.066	3.382ThrPro: 3.382 ± 0.053	1.538ThrGln: 1.538 ± 0.04	3.683ThrArg: 3.683 ± 0.063	2.474ThrSer: 2.474 ± 0.079	2.896ThrThr: 2.896 ± 0.096	4.362ThrVal: 4.362 ± 0.086	0.658ThrTrp: 0.658 ± 0.027	1.358ThrTyr: 1.358 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	8.538ValAla: 8.538 ± 0.101	0.612ValCys: 0.612 ± 0.028	4.425ValAsp: 4.425 ± 0.127	4.763ValGlu: 4.763 ± 0.072	2.814ValPhe: 2.814 ± 0.057	5.395ValGly: 5.395 ± 0.096	1.362ValHis: 1.362 ± 0.042	4.214ValIle: 4.214 ± 0.068	2.093ValLys: 2.093 ± 0.054	7.422ValLeu: 7.422 ± 0.095	2.064ValMet: 2.064 ± 0.055	1.944ValAsn: 1.944 ± 0.051	3.531ValPro: 3.531 ± 0.072	2.197ValGln: 2.197 ± 0.053	4.22ValArg: 4.22 ± 0.072	4.036ValSer: 4.036 ± 0.078	4.715ValThr: 4.715 ± 0.093	5.556ValVal: 5.556 ± 0.082	0.98ValTrp: 0.98 ± 0.041	1.503ValTyr: 1.503 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.487TrpAla: 1.487 ± 0.047	0.162TrpCys: 0.162 ± 0.014	0.839TrpAsp: 0.839 ± 0.034	0.693TrpGlu: 0.693 ± 0.027	0.612TrpPhe: 0.612 ± 0.024	1.031TrpGly: 1.031 ± 0.038	0.368TrpHis: 0.368 ± 0.021	0.695TrpIle: 0.695 ± 0.028	0.4TrpLys: 0.4 ± 0.022	1.685TrpLeu: 1.685 ± 0.046	0.4TrpMet: 0.4 ± 0.024	0.403TrpAsn: 0.403 ± 0.02	0.742TrpPro: 0.742 ± 0.032	0.64TrpGln: 0.64 ± 0.029	1.113TrpArg: 1.113 ± 0.039	0.797TrpSer: 0.797 ± 0.029	0.794TrpThr: 0.794 ± 0.03	0.953TrpVal: 0.953 ± 0.034	0.246TrpTrp: 0.246 ± 0.014	0.253TrpTyr: 0.253 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.521TyrAla: 2.521 ± 0.062	0.223TyrCys: 0.223 ± 0.016	1.693TyrAsp: 1.693 ± 0.045	1.405TyrGlu: 1.405 ± 0.039	0.879TyrPhe: 0.879 ± 0.03	2.072TyrGly: 2.072 ± 0.053	0.555TyrHis: 0.555 ± 0.024	0.939TyrIle: 0.939 ± 0.034	0.583TyrLys: 0.583 ± 0.025	2.213TyrLeu: 2.213 ± 0.051	0.53TyrMet: 0.53 ± 0.022	0.586TyrAsn: 0.586 ± 0.024	1.049TyrPro: 1.049 ± 0.033	0.742TyrGln: 0.742 ± 0.029	1.599TyrArg: 1.599 ± 0.044	1.096TyrSer: 1.096 ± 0.037	1.127TyrThr: 1.127 ± 0.042	1.5TyrVal: 1.5 ± 0.043	0.352TyrTrp: 0.352 ± 0.022	0.588TyrTyr: 0.588 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3136 proteins (979202 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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