Kingella potus
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2195 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A377R093|A0A377R093_9NEIS Staphylococcal nuclease homologue OS=Kingella potus OX=265175 GN=NCTC13336_00718 PE=4 SV=1
MM1 pKa = 7.31 GLCVVLQAYY10 pKa = 8.19 TEE12 pKa = 4.64 DD13 pKa = 4.5 DD14 pKa = 3.92 LNQILTGGSGFDD26 pKa = 3.22 ALQEE30 pKa = 4.15 EE31 pKa = 5.12 EE32 pKa = 3.97 NRR34 pKa = 11.84 AVAEE38 pKa = 4.15 CDD40 pKa = 4.06 LDD42 pKa = 4.67 KK43 pKa = 11.43 LWDD46 pKa = 3.81 CLRR49 pKa = 11.84 YY50 pKa = 9.97 ALTRR54 pKa = 11.84 SPEE57 pKa = 4.13 FEE59 pKa = 4.0 DD60 pKa = 4.06 TPLNNAVCGTHH71 pKa = 6.65 SVLEE75 pKa = 4.2 YY76 pKa = 10.93 VDD78 pKa = 5.45 LSPEE82 pKa = 3.86 EE83 pKa = 4.4 CAEE86 pKa = 4.31 LDD88 pKa = 3.61 PSEE91 pKa = 4.32 VPSYY95 pKa = 10.48 STVGDD100 pKa = 3.6 VAEE103 pKa = 4.99 ISAALNALDD112 pKa = 4.1 FAKK115 pKa = 10.61 QLGEE119 pKa = 3.57 ISAAEE124 pKa = 4.02 WEE126 pKa = 4.31 KK127 pKa = 11.25 SDD129 pKa = 4.62 VYY131 pKa = 10.99 RR132 pKa = 11.84 SDD134 pKa = 4.81 LFGDD138 pKa = 3.84 DD139 pKa = 4.03 PEE141 pKa = 5.33 IVDD144 pKa = 4.88 FLIEE148 pKa = 4.55 LFDD151 pKa = 3.9 EE152 pKa = 4.92 LRR154 pKa = 11.84 AFYY157 pKa = 8.82 QTAAEE162 pKa = 4.31 QKK164 pKa = 10.15 CGVLSTLCC172 pKa = 3.93
Molecular weight: 18.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.821
Patrickios 0.566
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A377QZ36|A0A377QZ36_9NEIS Uncharacterized protein OS=Kingella potus OX=265175 GN=NCTC13336_00014 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.87 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2195
0
2195
656480
30
10105
299.1
32.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.161 ± 0.086
1.087 ± 0.026
5.219 ± 0.04
5.818 ± 0.055
3.966 ± 0.042
7.882 ± 0.07
2.213 ± 0.029
5.127 ± 0.043
4.642 ± 0.05
10.214 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.274 ± 0.031
3.55 ± 0.051
4.622 ± 0.035
4.075 ± 0.043
6.211 ± 0.065
5.375 ± 0.049
5.021 ± 0.051
6.363 ± 0.056
1.285 ± 0.022
2.897 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here