Aliidiomarina minuta
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2751 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432W3H0|A0A432W3H0_9GAMM Uncharacterized protein OS=Aliidiomarina minuta OX=880057 GN=CWE09_12250 PE=4 SV=1
MM1 pKa = 7.9 EE2 pKa = 5.15 YY3 pKa = 8.87 NTSEE7 pKa = 4.51 LCDD10 pKa = 4.47 LYY12 pKa = 11.24 ADD14 pKa = 3.83 SVDD17 pKa = 3.49 VVEE20 pKa = 5.24 PMFVNYY26 pKa = 10.17 GGRR29 pKa = 11.84 SSFGGQIVTIKK40 pKa = 10.26 CHH42 pKa = 5.55 EE43 pKa = 4.66 DD44 pKa = 2.9 KK45 pKa = 11.42 GLIEE49 pKa = 4.79 ATLQDD54 pKa = 3.51 AGAGKK59 pKa = 10.45 VLLVDD64 pKa = 4.01 GGGSLRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.21 IAIAEE80 pKa = 4.24 LAVEE84 pKa = 4.47 NEE86 pKa = 3.9 WEE88 pKa = 4.51 GIICYY93 pKa = 10.48 GCVRR97 pKa = 11.84 DD98 pKa = 4.3 VDD100 pKa = 4.03 ALEE103 pKa = 5.17 DD104 pKa = 4.02 LDD106 pKa = 5.99 LGIQAVASLPVGAPSASTGEE126 pKa = 3.53 VDD128 pKa = 3.59 IPVNFGGVTFLPEE141 pKa = 3.46 DD142 pKa = 4.47 HH143 pKa = 7.26 IYY145 pKa = 11.47 ADD147 pKa = 3.57 ATGVILSPEE156 pKa = 4.24 PLDD159 pKa = 3.82 IDD161 pKa = 3.71
Molecular weight: 16.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.859
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.859
Patrickios 0.985
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A432WA08|A0A432WA08_9GAMM Uncharacterized protein OS=Aliidiomarina minuta OX=880057 GN=CWE09_09025 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2751
0
2751
902465
37
2816
328.0
36.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.354 ± 0.046
0.852 ± 0.015
5.711 ± 0.035
6.652 ± 0.049
3.916 ± 0.033
6.995 ± 0.042
2.318 ± 0.023
5.645 ± 0.038
3.879 ± 0.053
10.776 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.023
3.672 ± 0.03
4.146 ± 0.024
5.359 ± 0.047
5.756 ± 0.036
6.321 ± 0.032
4.937 ± 0.028
6.86 ± 0.042
1.36 ± 0.021
2.939 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here