Pseudomonas virus PBPA162

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TNB4|A0A4Y5TNB4_9CAUD GTP cyclohydrolase I OS=Pseudomonas virus PBPA162 OX=2588096 GN=folE PE=3 SV=1
MM1 pKa = 7.33TEE3 pKa = 3.81YY4 pKa = 10.9AGLYY8 pKa = 8.69TPVNFVDD15 pKa = 4.38PPTPSGLPEE24 pKa = 4.96DD25 pKa = 4.34LTPVGYY31 pKa = 8.48ITHH34 pKa = 7.17DD35 pKa = 3.21EE36 pKa = 4.18RR37 pKa = 11.84GFITGSMFCQPRR49 pKa = 11.84CVEE52 pKa = 4.23SNAMGRR58 pKa = 11.84LYY60 pKa = 10.6LRR62 pKa = 11.84HH63 pKa = 6.83DD64 pKa = 3.96NYY66 pKa = 11.19SASKK70 pKa = 10.13ALWYY74 pKa = 9.83VQGGALVARR83 pKa = 11.84PMLPYY88 pKa = 9.96TLRR91 pKa = 11.84EE92 pKa = 3.92NVLVGVPQGATLRR105 pKa = 11.84IEE107 pKa = 4.31GVEE110 pKa = 4.04YY111 pKa = 9.28TADD114 pKa = 3.5GTDD117 pKa = 3.13IEE119 pKa = 5.4LSFSHH124 pKa = 6.62SGTFIVEE131 pKa = 4.01VDD133 pKa = 3.9PFPYY137 pKa = 9.75QAFEE141 pKa = 4.33VEE143 pKa = 4.55VEE145 pKa = 4.34YY146 pKa = 10.39EE147 pKa = 4.4APTQSS152 pKa = 3.07

Molecular weight: 16.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TNE0|A0A4Y5TNE0_9CAUD Uncharacterized protein OS=Pseudomonas virus PBPA162 OX=2588096 PE=4 SV=1
MM1 pKa = 6.92QHH3 pKa = 5.85TEE5 pKa = 4.1RR6 pKa = 11.84EE7 pKa = 4.3SLRR10 pKa = 11.84AVAAMVDD17 pKa = 3.6QAIEE21 pKa = 3.89KK22 pKa = 9.79CIADD26 pKa = 3.77MRR28 pKa = 11.84PFSLLPVSKK37 pKa = 10.41LDD39 pKa = 3.19AVTYY43 pKa = 10.16MFDD46 pKa = 3.46APGAAWRR53 pKa = 11.84TVVTRR58 pKa = 11.84EE59 pKa = 3.57QRR61 pKa = 11.84VAARR65 pKa = 11.84GVTRR69 pKa = 11.84EE70 pKa = 4.04LATKK74 pKa = 10.07RR75 pKa = 11.84LMVGLVGAAAQAVLISNAYY94 pKa = 8.12TPRR97 pKa = 11.84QAQYY101 pKa = 10.97AASQAAWARR110 pKa = 11.84RR111 pKa = 11.84WLVTLL116 pKa = 4.56

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

18612

32

1291

224.2

24.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.913 ± 0.79

1.499 ± 0.188

5.319 ± 0.163

6.216 ± 0.294

3.052 ± 0.162

7.484 ± 0.252

2.343 ± 0.241

4.513 ± 0.193

5.056 ± 0.255

8.532 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.106 ± 0.18

4.245 ± 0.233

4.712 ± 0.34

4.857 ± 0.3

5.856 ± 0.218

5.179 ± 0.16

5.539 ± 0.293

7.554 ± 0.212

1.623 ± 0.129

3.401 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski