Amino acid dipepetide frequency for Pseudomonas virus PBPA162

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.218AlaAla: 13.218 ± 2.344
1.236AlaCys: 1.236 ± 0.334
4.513AlaAsp: 4.513 ± 0.418
6.77AlaGlu: 6.77 ± 1.128
2.579AlaPhe: 2.579 ± 0.369
6.555AlaGly: 6.555 ± 0.921
1.236AlaHis: 1.236 ± 0.286
5.588AlaIle: 5.588 ± 0.468
5.696AlaLys: 5.696 ± 0.738
8.113AlaLeu: 8.113 ± 0.758
3.17AlaMet: 3.17 ± 0.535
3.869AlaAsn: 3.869 ± 0.63
4.245AlaPro: 4.245 ± 0.674
5.481AlaGln: 5.481 ± 0.964
5.158AlaArg: 5.158 ± 0.578
4.675AlaSer: 4.675 ± 0.463
6.018AlaThr: 6.018 ± 1.01
6.824AlaVal: 6.824 ± 0.568
1.021AlaTrp: 1.021 ± 0.231
3.17AlaTyr: 3.17 ± 0.393
0.0AlaXaa: 0.0 ± 0.0
Cys
0.645CysAla: 0.645 ± 0.186
0.376CysCys: 0.376 ± 0.155
1.021CysAsp: 1.021 ± 0.282
0.967CysGlu: 0.967 ± 0.232
0.591CysPhe: 0.591 ± 0.211
1.343CysGly: 1.343 ± 0.243
0.591CysHis: 0.591 ± 0.211
0.913CysIle: 0.913 ± 0.208
0.591CysLys: 0.591 ± 0.153
1.612CysLeu: 1.612 ± 0.325
0.376CysMet: 0.376 ± 0.163
0.913CysAsn: 0.913 ± 0.207
0.591CysPro: 0.591 ± 0.171
0.913CysGln: 0.913 ± 0.208
0.645CysArg: 0.645 ± 0.188
0.645CysSer: 0.645 ± 0.185
0.806CysThr: 0.806 ± 0.258
1.128CysVal: 1.128 ± 0.228
0.161CysTrp: 0.161 ± 0.102
0.161CysTyr: 0.161 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
5.051AspAla: 5.051 ± 0.566
0.645AspCys: 0.645 ± 0.171
2.633AspAsp: 2.633 ± 0.364
3.707AspGlu: 3.707 ± 0.508
2.149AspPhe: 2.149 ± 0.287
5.964AspGly: 5.964 ± 0.607
1.128AspHis: 1.128 ± 0.271
2.525AspIle: 2.525 ± 0.313
2.418AspLys: 2.418 ± 0.327
4.782AspLeu: 4.782 ± 0.534
1.343AspMet: 1.343 ± 0.232
1.988AspAsn: 1.988 ± 0.29
3.116AspPro: 3.116 ± 0.366
1.988AspGln: 1.988 ± 0.302
2.579AspArg: 2.579 ± 0.376
1.881AspSer: 1.881 ± 0.321
2.31AspThr: 2.31 ± 0.281
3.976AspVal: 3.976 ± 0.509
0.913AspTrp: 0.913 ± 0.184
2.096AspTyr: 2.096 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
6.34GluAla: 6.34 ± 0.799
0.806GluCys: 0.806 ± 0.262
2.74GluAsp: 2.74 ± 0.335
3.546GluGlu: 3.546 ± 0.553
2.257GluPhe: 2.257 ± 0.37
3.869GluGly: 3.869 ± 0.469
1.504GluHis: 1.504 ± 0.314
2.579GluIle: 2.579 ± 0.282
3.17GluLys: 3.17 ± 0.546
7.2GluLeu: 7.2 ± 0.558
2.257GluMet: 2.257 ± 0.31
1.934GluAsn: 1.934 ± 0.267
2.687GluPro: 2.687 ± 0.425
3.439GluGln: 3.439 ± 0.474
3.976GluArg: 3.976 ± 0.588
3.17GluSer: 3.17 ± 0.446
2.31GluThr: 2.31 ± 0.321
4.352GluVal: 4.352 ± 0.424
1.343GluTrp: 1.343 ± 0.234
2.687GluTyr: 2.687 ± 0.447
0.0GluXaa: 0.0 ± 0.0
Phe
1.934PheAla: 1.934 ± 0.397
0.376PheCys: 0.376 ± 0.163
2.257PheAsp: 2.257 ± 0.341
2.096PheGlu: 2.096 ± 0.247
0.537PhePhe: 0.537 ± 0.159
3.009PheGly: 3.009 ± 0.402
0.806PheHis: 0.806 ± 0.234
1.612PheIle: 1.612 ± 0.333
1.881PheLys: 1.881 ± 0.336
2.364PheLeu: 2.364 ± 0.318
1.021PheMet: 1.021 ± 0.254
2.042PheAsn: 2.042 ± 0.317
1.236PhePro: 1.236 ± 0.275
1.128PheGln: 1.128 ± 0.214
1.719PheArg: 1.719 ± 0.336
1.558PheSer: 1.558 ± 0.272
1.827PheThr: 1.827 ± 0.274
1.881PheVal: 1.881 ± 0.249
0.43PheTrp: 0.43 ± 0.145
0.806PheTyr: 0.806 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
6.77GlyAla: 6.77 ± 0.658
1.021GlyCys: 1.021 ± 0.189
3.493GlyAsp: 3.493 ± 0.394
4.245GlyGlu: 4.245 ± 0.336
2.418GlyPhe: 2.418 ± 0.369
6.125GlyGly: 6.125 ± 0.696
1.504GlyHis: 1.504 ± 0.316
2.955GlyIle: 2.955 ± 0.37
4.46GlyLys: 4.46 ± 0.644
6.502GlyLeu: 6.502 ± 0.593
2.848GlyMet: 2.848 ± 0.382
3.009GlyAsn: 3.009 ± 0.391
2.848GlyPro: 2.848 ± 0.337
3.6GlyGln: 3.6 ± 0.397
4.46GlyArg: 4.46 ± 0.394
3.654GlySer: 3.654 ± 0.523
4.299GlyThr: 4.299 ± 0.39
6.77GlyVal: 6.77 ± 0.439
0.967GlyTrp: 0.967 ± 0.254
2.902GlyTyr: 2.902 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.881HisAla: 1.881 ± 0.296
0.645HisCys: 0.645 ± 0.259
0.967HisAsp: 0.967 ± 0.223
0.86HisGlu: 0.86 ± 0.189
0.913HisPhe: 0.913 ± 0.205
1.719HisGly: 1.719 ± 0.353
0.806HisHis: 0.806 ± 0.229
1.397HisIle: 1.397 ± 0.264
1.558HisLys: 1.558 ± 0.357
1.988HisLeu: 1.988 ± 0.394
0.913HisMet: 0.913 ± 0.26
1.021HisAsn: 1.021 ± 0.231
1.29HisPro: 1.29 ± 0.275
1.021HisGln: 1.021 ± 0.226
0.967HisArg: 0.967 ± 0.215
1.236HisSer: 1.236 ± 0.202
1.558HisThr: 1.558 ± 0.346
1.451HisVal: 1.451 ± 0.33
0.484HisTrp: 0.484 ± 0.188
0.752HisTyr: 0.752 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
4.943IleAla: 4.943 ± 0.493
0.645IleCys: 0.645 ± 0.185
3.063IleAsp: 3.063 ± 0.39
2.902IleGlu: 2.902 ± 0.339
1.236IlePhe: 1.236 ± 0.275
3.869IleGly: 3.869 ± 0.462
0.591IleHis: 0.591 ± 0.152
1.719IleIle: 1.719 ± 0.361
2.364IleLys: 2.364 ± 0.37
2.687IleLeu: 2.687 ± 0.369
1.128IleMet: 1.128 ± 0.247
2.364IleAsn: 2.364 ± 0.354
2.525IlePro: 2.525 ± 0.358
2.257IleGln: 2.257 ± 0.334
2.149IleArg: 2.149 ± 0.309
2.687IleSer: 2.687 ± 0.353
3.224IleThr: 3.224 ± 0.564
3.17IleVal: 3.17 ± 0.401
0.43IleTrp: 0.43 ± 0.128
1.182IleTyr: 1.182 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
5.534LysAla: 5.534 ± 0.844
0.913LysCys: 0.913 ± 0.244
2.579LysAsp: 2.579 ± 0.437
3.063LysGlu: 3.063 ± 0.487
1.397LysPhe: 1.397 ± 0.29
3.331LysGly: 3.331 ± 0.45
0.591LysHis: 0.591 ± 0.158
2.042LysIle: 2.042 ± 0.252
2.955LysLys: 2.955 ± 0.459
5.051LysLeu: 5.051 ± 0.68
1.988LysMet: 1.988 ± 0.301
1.29LysAsn: 1.29 ± 0.257
2.472LysPro: 2.472 ± 0.375
1.827LysGln: 1.827 ± 0.362
3.439LysArg: 3.439 ± 0.423
3.116LysSer: 3.116 ± 0.363
2.31LysThr: 2.31 ± 0.38
4.245LysVal: 4.245 ± 0.502
0.537LysTrp: 0.537 ± 0.149
1.881LysTyr: 1.881 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
8.436LeuAla: 8.436 ± 1.01
1.612LeuCys: 1.612 ± 0.3
5.857LeuAsp: 5.857 ± 0.642
5.749LeuGlu: 5.749 ± 0.513
2.794LeuPhe: 2.794 ± 0.383
5.803LeuGly: 5.803 ± 0.662
2.149LeuHis: 2.149 ± 0.347
3.546LeuIle: 3.546 ± 0.433
3.17LeuLys: 3.17 ± 0.389
6.287LeuLeu: 6.287 ± 0.631
1.934LeuMet: 1.934 ± 0.312
3.439LeuAsn: 3.439 ± 0.427
4.513LeuPro: 4.513 ± 0.508
3.654LeuGln: 3.654 ± 0.455
6.179LeuArg: 6.179 ± 0.743
5.481LeuSer: 5.481 ± 0.502
4.997LeuThr: 4.997 ± 0.494
6.072LeuVal: 6.072 ± 0.712
0.913LeuTrp: 0.913 ± 0.159
2.74LeuTyr: 2.74 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.385
0.484MetCys: 0.484 ± 0.16
1.504MetAsp: 1.504 ± 0.212
2.042MetGlu: 2.042 ± 0.349
1.128MetPhe: 1.128 ± 0.245
1.827MetGly: 1.827 ± 0.36
1.29MetHis: 1.29 ± 0.209
0.913MetIle: 0.913 ± 0.217
1.021MetLys: 1.021 ± 0.257
2.579MetLeu: 2.579 ± 0.377
0.43MetMet: 0.43 ± 0.151
1.236MetAsn: 1.236 ± 0.219
0.967MetPro: 0.967 ± 0.224
1.504MetGln: 1.504 ± 0.271
2.579MetArg: 2.579 ± 0.394
2.418MetSer: 2.418 ± 0.309
2.472MetThr: 2.472 ± 0.311
2.579MetVal: 2.579 ± 0.46
0.537MetTrp: 0.537 ± 0.152
1.182MetTyr: 1.182 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.922AsnAla: 3.922 ± 0.456
0.645AsnCys: 0.645 ± 0.173
2.257AsnAsp: 2.257 ± 0.319
1.666AsnGlu: 1.666 ± 0.268
0.967AsnPhe: 0.967 ± 0.217
3.493AsnGly: 3.493 ± 0.446
1.29AsnHis: 1.29 ± 0.296
2.364AsnIle: 2.364 ± 0.446
1.719AsnLys: 1.719 ± 0.308
4.406AsnLeu: 4.406 ± 0.553
1.182AsnMet: 1.182 ± 0.232
2.472AsnAsn: 2.472 ± 0.468
2.418AsnPro: 2.418 ± 0.349
2.096AsnGln: 2.096 ± 0.278
2.149AsnArg: 2.149 ± 0.292
2.096AsnSer: 2.096 ± 0.389
1.881AsnThr: 1.881 ± 0.255
3.009AsnVal: 3.009 ± 0.409
1.075AsnTrp: 1.075 ± 0.189
1.29AsnTyr: 1.29 ± 0.334
0.0AsnXaa: 0.0 ± 0.0
Pro
3.976ProAla: 3.976 ± 0.438
0.591ProCys: 0.591 ± 0.193
3.224ProAsp: 3.224 ± 0.399
3.761ProGlu: 3.761 ± 0.676
1.773ProPhe: 1.773 ± 0.358
4.084ProGly: 4.084 ± 0.511
1.29ProHis: 1.29 ± 0.249
1.988ProIle: 1.988 ± 0.335
2.042ProLys: 2.042 ± 0.328
3.439ProLeu: 3.439 ± 0.539
0.967ProMet: 0.967 ± 0.222
1.827ProAsn: 1.827 ± 0.366
1.988ProPro: 1.988 ± 0.362
2.31ProGln: 2.31 ± 0.381
1.988ProArg: 1.988 ± 0.268
2.149ProSer: 2.149 ± 0.284
2.794ProThr: 2.794 ± 0.57
3.869ProVal: 3.869 ± 0.63
1.343ProTrp: 1.343 ± 0.288
1.719ProTyr: 1.719 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
6.502GlnAla: 6.502 ± 0.81
0.86GlnCys: 0.86 ± 0.218
2.042GlnAsp: 2.042 ± 0.33
3.009GlnGlu: 3.009 ± 0.499
1.021GlnPhe: 1.021 ± 0.2
3.815GlnGly: 3.815 ± 0.446
1.451GlnHis: 1.451 ± 0.271
2.149GlnIle: 2.149 ± 0.53
1.666GlnLys: 1.666 ± 0.301
3.546GlnLeu: 3.546 ± 0.366
1.451GlnMet: 1.451 ± 0.244
1.612GlnAsn: 1.612 ± 0.32
2.74GlnPro: 2.74 ± 0.467
2.203GlnGln: 2.203 ± 0.514
3.331GlnArg: 3.331 ± 0.494
2.257GlnSer: 2.257 ± 0.334
2.579GlnThr: 2.579 ± 0.381
3.922GlnVal: 3.922 ± 0.524
0.752GlnTrp: 0.752 ± 0.216
1.666GlnTyr: 1.666 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
5.749ArgAla: 5.749 ± 0.589
0.537ArgCys: 0.537 ± 0.136
2.794ArgAsp: 2.794 ± 0.376
3.493ArgGlu: 3.493 ± 0.479
2.203ArgPhe: 2.203 ± 0.349
3.546ArgGly: 3.546 ± 0.45
1.666ArgHis: 1.666 ± 0.412
2.955ArgIle: 2.955 ± 0.338
3.439ArgLys: 3.439 ± 0.415
5.373ArgLeu: 5.373 ± 0.614
2.472ArgMet: 2.472 ± 0.342
2.633ArgAsn: 2.633 ± 0.336
3.063ArgPro: 3.063 ± 0.494
2.902ArgGln: 2.902 ± 0.345
2.848ArgArg: 2.848 ± 0.393
3.063ArgSer: 3.063 ± 0.363
2.525ArgThr: 2.525 ± 0.344
4.245ArgVal: 4.245 ± 0.496
1.29ArgTrp: 1.29 ± 0.284
1.773ArgTyr: 1.773 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
4.03SerAla: 4.03 ± 0.485
0.806SerCys: 0.806 ± 0.236
2.902SerAsp: 2.902 ± 0.327
2.364SerGlu: 2.364 ± 0.347
1.934SerPhe: 1.934 ± 0.323
4.46SerGly: 4.46 ± 0.539
0.967SerHis: 0.967 ± 0.248
2.149SerIle: 2.149 ± 0.321
3.331SerLys: 3.331 ± 0.32
4.46SerLeu: 4.46 ± 0.577
1.988SerMet: 1.988 ± 0.353
2.633SerAsn: 2.633 ± 0.356
1.827SerPro: 1.827 ± 0.247
2.955SerGln: 2.955 ± 0.369
3.116SerArg: 3.116 ± 0.378
2.848SerSer: 2.848 ± 0.418
2.579SerThr: 2.579 ± 0.366
3.761SerVal: 3.761 ± 0.458
0.86SerTrp: 0.86 ± 0.24
1.827SerTyr: 1.827 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
5.427ThrAla: 5.427 ± 0.935
0.591ThrCys: 0.591 ± 0.206
2.955ThrAsp: 2.955 ± 0.373
3.922ThrGlu: 3.922 ± 0.558
1.666ThrPhe: 1.666 ± 0.262
3.922ThrGly: 3.922 ± 0.42
1.558ThrHis: 1.558 ± 0.252
2.579ThrIle: 2.579 ± 0.43
2.257ThrLys: 2.257 ± 0.423
4.728ThrLeu: 4.728 ± 0.443
1.612ThrMet: 1.612 ± 0.251
2.902ThrAsn: 2.902 ± 0.317
3.439ThrPro: 3.439 ± 0.588
2.902ThrGln: 2.902 ± 0.33
2.74ThrArg: 2.74 ± 0.327
2.472ThrSer: 2.472 ± 0.472
3.063ThrThr: 3.063 ± 0.6
3.707ThrVal: 3.707 ± 0.493
1.128ThrTrp: 1.128 ± 0.31
1.827ThrTyr: 1.827 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
7.791ValAla: 7.791 ± 0.634
1.236ValCys: 1.236 ± 0.299
3.922ValAsp: 3.922 ± 0.443
5.319ValGlu: 5.319 ± 0.502
1.558ValPhe: 1.558 ± 0.372
5.319ValGly: 5.319 ± 0.546
1.666ValHis: 1.666 ± 0.282
3.063ValIle: 3.063 ± 0.313
4.137ValLys: 4.137 ± 0.51
5.857ValLeu: 5.857 ± 0.607
2.149ValMet: 2.149 ± 0.345
2.687ValAsn: 2.687 ± 0.384
3.009ValPro: 3.009 ± 0.379
4.513ValGln: 4.513 ± 0.558
4.836ValArg: 4.836 ± 0.457
3.385ValSer: 3.385 ± 0.362
4.89ValThr: 4.89 ± 0.566
6.609ValVal: 6.609 ± 0.632
1.021ValTrp: 1.021 ± 0.276
2.579ValTyr: 2.579 ± 0.443
0.0ValXaa: 0.0 ± 0.0
Trp
1.612TrpAla: 1.612 ± 0.291
0.322TrpCys: 0.322 ± 0.123
1.021TrpAsp: 1.021 ± 0.209
0.806TrpGlu: 0.806 ± 0.194
0.322TrpPhe: 0.322 ± 0.142
0.699TrpGly: 0.699 ± 0.163
0.537TrpHis: 0.537 ± 0.181
0.484TrpIle: 0.484 ± 0.165
0.645TrpLys: 0.645 ± 0.164
1.451TrpLeu: 1.451 ± 0.236
0.537TrpMet: 0.537 ± 0.142
0.86TrpAsn: 0.86 ± 0.188
1.021TrpPro: 1.021 ± 0.189
0.43TrpGln: 0.43 ± 0.135
1.236TrpArg: 1.236 ± 0.23
0.86TrpSer: 0.86 ± 0.259
1.075TrpThr: 1.075 ± 0.22
1.128TrpVal: 1.128 ± 0.255
0.43TrpTrp: 0.43 ± 0.226
0.752TrpTyr: 0.752 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.009TyrAla: 3.009 ± 0.359
0.645TyrCys: 0.645 ± 0.191
1.451TyrAsp: 1.451 ± 0.311
1.881TyrGlu: 1.881 ± 0.35
1.075TyrPhe: 1.075 ± 0.231
2.096TyrGly: 2.096 ± 0.359
0.806TyrHis: 0.806 ± 0.215
1.612TyrIle: 1.612 ± 0.267
2.042TyrLys: 2.042 ± 0.347
2.902TyrLeu: 2.902 ± 0.363
1.236TyrMet: 1.236 ± 0.232
1.719TyrAsn: 1.719 ± 0.375
1.128TyrPro: 1.128 ± 0.271
1.451TyrGln: 1.451 ± 0.272
2.472TyrArg: 2.472 ± 0.309
2.149TyrSer: 2.149 ± 0.324
1.881TyrThr: 1.881 ± 0.314
2.848TyrVal: 2.848 ± 0.324
0.591TyrTrp: 0.591 ± 0.19
1.021TyrTyr: 1.021 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (18612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski