Capybara microvirus Cap3_SP_449
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W530|A0A4P8W530_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_449 OX=2585459 PE=4 SV=1
MM1 pKa = 7.69 TYY3 pKa = 8.64 FTIKK7 pKa = 10.25 DD8 pKa = 3.64 DD9 pKa = 3.64 KK10 pKa = 10.24 TGSFEE15 pKa = 4.75 GLFPFPNIEE24 pKa = 3.88 VAKK27 pKa = 10.49 RR28 pKa = 11.84 NLGYY32 pKa = 11.21 ALTHH36 pKa = 5.7 KK37 pKa = 10.41 QMPEE41 pKa = 3.65 IMCEE45 pKa = 4.25 FPADD49 pKa = 3.68 YY50 pKa = 11.07 QLFLVGDD57 pKa = 4.86 LDD59 pKa = 4.06 PKK61 pKa = 10.56 TGVFSPDD68 pKa = 2.99 QKK70 pKa = 11.1 YY71 pKa = 9.05 VCCLLEE77 pKa = 4.16 CVNGQSSPNN86 pKa = 3.38
Molecular weight: 9.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.406
IPC2_protein 4.762
IPC_protein 4.571
Toseland 4.406
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.431
Rodwell 4.406
Grimsley 4.317
Solomon 4.52
Lehninger 4.482
Nozaki 4.647
DTASelect 4.825
Thurlkill 4.431
EMBOSS 4.444
Sillero 4.685
Patrickios 2.041
IPC_peptide 4.533
IPC2_peptide 4.673
IPC2.peptide.svr19 4.652
Protein with the highest isoelectric point:
>tr|A0A4P8W5Y9|A0A4P8W5Y9_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_449 OX=2585459 PE=4 SV=1
MM1 pKa = 7.6 SYY3 pKa = 10.95 AAGEE7 pKa = 4.36 GSRR10 pKa = 11.84 GVASRR15 pKa = 11.84 VLGRR19 pKa = 11.84 KK20 pKa = 8.71 GPKK23 pKa = 9.52 GRR25 pKa = 11.84 QTRR28 pKa = 11.84 AAQAAQKK35 pKa = 10.21 NKK37 pKa = 10.06 KK38 pKa = 9.79 SEE40 pKa = 4.0 EE41 pKa = 4.05 NVRR44 pKa = 11.84 GGHH47 pKa = 6.49 CSPDD51 pKa = 2.93 SRR53 pKa = 11.84 QAGRR57 pKa = 11.84 RR58 pKa = 11.84 SHH60 pKa = 6.4 EE61 pKa = 4.39 KK62 pKa = 10.36 RR63 pKa = 11.84 DD64 pKa = 3.86 DD65 pKa = 4.24 DD66 pKa = 5.92 LNDD69 pKa = 3.5 DD70 pKa = 4.34 AKK72 pKa = 10.93 NVRR75 pKa = 11.84 IGYY78 pKa = 9.11 LRR80 pKa = 11.84 AARR83 pKa = 11.84 RR84 pKa = 3.45
Molecular weight: 9.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.765
IPC_protein 10.804
Toseland 11.067
ProMoST 11.316
Dawson 11.111
Bjellqvist 10.891
Wikipedia 11.389
Rodwell 11.213
Grimsley 11.14
Solomon 11.359
Lehninger 11.316
Nozaki 11.038
DTASelect 10.891
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.052
Patrickios 10.95
IPC_peptide 11.374
IPC2_peptide 10.072
IPC2.peptide.svr19 8.915
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1565
84
558
195.6
21.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.179 ± 1.062
1.534 ± 0.535
6.134 ± 0.334
6.645 ± 1.662
4.089 ± 0.642
7.54 ± 0.583
2.045 ± 0.301
5.176 ± 0.556
6.518 ± 1.59
8.243 ± 0.678
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.173 ± 0.276
4.409 ± 0.466
4.473 ± 0.94
3.898 ± 0.506
5.112 ± 1.009
7.348 ± 1.12
6.134 ± 0.987
4.984 ± 0.667
1.597 ± 0.434
3.77 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here