Cytophagaceae bacterium SJW1-29

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; unclassified Cytophagaceae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9B9M3|A0A7C9B9M3_9BACT DinB family protein OS=Cytophagaceae bacterium SJW1-29 OX=2654236 GN=GBK04_08815 PE=4 SV=1
MM1 pKa = 7.41VDD3 pKa = 3.38GVTVAVVNSDD13 pKa = 3.14STLTIKK19 pKa = 11.01GPGSYY24 pKa = 9.09TFLSTSGVLYY34 pKa = 9.42PASGFCAIEE43 pKa = 4.07VVPGAFFDD51 pKa = 4.22LALSKK56 pKa = 10.9SLAPGQASTVTAGSLVKK73 pKa = 10.28FRR75 pKa = 11.84LTVSNEE81 pKa = 3.59GNMEE85 pKa = 4.24ALQIALSDD93 pKa = 3.86SLPEE97 pKa = 4.23GMTLADD103 pKa = 4.02TSWTASGGFATLNVPLAGPLYY124 pKa = 9.86PGATAFIDD132 pKa = 3.03ITLQVDD138 pKa = 3.42SSFTSGTLTNFAQIKK153 pKa = 9.52DD154 pKa = 3.77ASCATDD160 pKa = 3.78EE161 pKa = 4.29PVIDD165 pKa = 4.86IDD167 pKa = 3.92STPDD171 pKa = 2.93NGFNNGEE178 pKa = 4.46DD179 pKa = 4.13DD180 pKa = 5.27DD181 pKa = 5.54DD182 pKa = 5.46SEE184 pKa = 5.41SISISQCPTITLTTSSDD201 pKa = 3.2KK202 pKa = 11.03VICEE206 pKa = 4.23GEE208 pKa = 4.45SVVLNVTASEE218 pKa = 4.21SGATIDD224 pKa = 3.79WYY226 pKa = 9.52TVPTGGTPFVTTASEE241 pKa = 4.12ADD243 pKa = 3.47QTLSPTQTTTYY254 pKa = 9.61YY255 pKa = 10.92VEE257 pKa = 5.81GSLQDD262 pKa = 3.44DD263 pKa = 4.17CKK265 pKa = 10.62SARR268 pKa = 11.84VPITVTVNAKK278 pKa = 8.69PTIPVAPGNIQNICPDD294 pKa = 3.41TTADD298 pKa = 3.7LTTIDD303 pKa = 4.61LTASTPGGFFEE314 pKa = 4.52WRR316 pKa = 11.84EE317 pKa = 4.05GMLSTSALVDD327 pKa = 3.84DD328 pKa = 4.35PTKK331 pKa = 11.0VEE333 pKa = 3.81AGTYY337 pKa = 7.16YY338 pKa = 9.83ICEE341 pKa = 4.17KK342 pKa = 10.65SVEE345 pKa = 4.11GCYY348 pKa = 10.71GSAQAVVVNIVPCDD362 pKa = 3.64CQLEE366 pKa = 4.26YY367 pKa = 11.09SVAAGVDD374 pKa = 3.58QEE376 pKa = 4.65VCAGMPISVTATTSGTVSGIIWTTSGTGTFANADD410 pKa = 4.1SLNTTYY416 pKa = 10.39TPSAADD422 pKa = 3.16IAAGEE427 pKa = 4.18IALTVTTNDD436 pKa = 3.42PDD438 pKa = 5.37GDD440 pKa = 4.09DD441 pKa = 3.65TCVPKK446 pKa = 10.03MDD448 pKa = 4.67ALTVVIIPQPAPAYY462 pKa = 10.08GVACDD467 pKa = 4.17DD468 pKa = 3.87TLICVGKK475 pKa = 8.25STKK478 pKa = 10.6LLGFAPGYY486 pKa = 7.85TINWYY491 pKa = 5.8TTPTGGTPIGTTPSGGKK508 pKa = 8.31LTVSPSATTTYY519 pKa = 9.65YY520 pKa = 11.23AEE522 pKa = 5.05AIGDD526 pKa = 3.86KK527 pKa = 10.51GCSSEE532 pKa = 3.92EE533 pKa = 3.91RR534 pKa = 11.84TPVTVVVQPCSSDD547 pKa = 3.27LAIVKK552 pKa = 8.46TVLTPAPYY560 pKa = 10.25SAGQEE565 pKa = 3.86INYY568 pKa = 10.03SLTVTSLAVGNASNVTVEE586 pKa = 4.43DD587 pKa = 3.77VLPASLLYY595 pKa = 10.44VASAPAGEE603 pKa = 4.52YY604 pKa = 9.86NAEE607 pKa = 4.13TGVWTIGNMINGSNRR622 pKa = 11.84VLVITAKK629 pKa = 10.39IQDD632 pKa = 3.99NASGDD637 pKa = 3.53ITNTAIVKK645 pKa = 10.64SPDD648 pKa = 3.34NDD650 pKa = 3.93PGNTSNDD657 pKa = 3.45TSSVTIRR664 pKa = 11.84VGNLADD670 pKa = 4.32LMLAKK675 pKa = 10.09KK676 pKa = 10.56VSTVNPALGEE686 pKa = 4.47SITYY690 pKa = 8.36TLEE693 pKa = 3.7VSNNGPQRR701 pKa = 11.84ATNIEE706 pKa = 4.1VTDD709 pKa = 3.67QLPAGLEE716 pKa = 4.45FVSSTDD722 pKa = 3.72FSKK725 pKa = 9.8TGNLLKK731 pKa = 10.32GTIDD735 pKa = 3.76SIEE738 pKa = 4.14VGDD741 pKa = 4.05TKK743 pKa = 10.93TLTFLAKK750 pKa = 9.95VVSGTSITNIAQISKK765 pKa = 10.31SDD767 pKa = 3.49QKK769 pKa = 11.61DD770 pKa = 3.41PDD772 pKa = 3.82STPGNGYY779 pKa = 10.97SNGEE783 pKa = 4.0DD784 pKa = 3.81DD785 pKa = 4.25EE786 pKa = 6.63ASVTVNTGCPTIDD799 pKa = 3.79PPIIACAQTTICVGTSVTLTAVGCKK824 pKa = 10.04NGTVKK829 pKa = 10.32WSNGMEE835 pKa = 4.18GVSITLPIDD844 pKa = 3.11QTTTFTAVCQKK855 pKa = 10.64DD856 pKa = 3.67EE857 pKa = 4.59CNSIPSNPITINVANTVKK875 pKa = 10.35PVLASNVSSVCEE887 pKa = 4.21GGSATLTAANCNGVLVWSTGATGTPWW913 pKa = 3.32

Molecular weight: 93.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9BLC4|A0A7C9BLC4_9BACT Uncharacterized protein OS=Cytophagaceae bacterium SJW1-29 OX=2654236 GN=GBK04_26170 PE=4 SV=1
MM1 pKa = 7.84LITHH5 pKa = 6.35TTDD8 pKa = 3.34QQVCCPLCQQVSSRR22 pKa = 11.84LHH24 pKa = 4.89GFYY27 pKa = 10.34YY28 pKa = 10.49RR29 pKa = 11.84KK30 pKa = 8.74PTDD33 pKa = 3.68LFIGDD38 pKa = 3.94KK39 pKa = 10.22QVQLRR44 pKa = 11.84VRR46 pKa = 11.84LRR48 pKa = 11.84RR49 pKa = 11.84FRR51 pKa = 11.84CLNSVRR57 pKa = 11.84PKK59 pKa = 9.76RR60 pKa = 11.84TFGQPCPDD68 pKa = 2.91WLFTFFHH75 pKa = 6.06RR76 pKa = 11.84TSLLVHH82 pKa = 6.33AQRR85 pKa = 11.84NVAMVLGGKK94 pKa = 8.25TSARR98 pKa = 11.84LLIHH102 pKa = 6.51LHH104 pKa = 5.9MLTSHH109 pKa = 6.81DD110 pKa = 3.55TLVRR114 pKa = 11.84IIRR117 pKa = 11.84KK118 pKa = 5.41WQPVDD123 pKa = 3.46LQTPHH128 pKa = 7.13ALSVDD133 pKa = 2.91DD134 pKa = 3.74WAIRR138 pKa = 11.84KK139 pKa = 8.97AKK141 pKa = 9.84TYY143 pKa = 8.97GTILTVRR150 pKa = 11.84RR151 pKa = 11.84YY152 pKa = 9.91DD153 pKa = 3.18

Molecular weight: 17.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5629

0

5629

1951742

20

2550

346.7

38.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.848 ± 0.034

0.751 ± 0.011

5.388 ± 0.023

5.921 ± 0.034

4.652 ± 0.026

7.258 ± 0.034

1.888 ± 0.018

5.951 ± 0.027

5.555 ± 0.03

9.986 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.013

4.646 ± 0.028

4.485 ± 0.022

3.99 ± 0.02

5.164 ± 0.027

6.175 ± 0.025

6.041 ± 0.032

6.685 ± 0.025

1.381 ± 0.014

4.0 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski