Amino acid dipepetide frequency for Cytophagaceae bacterium SJW1-29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.685AlaAla: 6.685 ± 0.077	0.718AlaCys: 0.718 ± 0.02	4.566AlaAsp: 4.566 ± 0.056	4.938AlaGlu: 4.938 ± 0.064	3.528AlaPhe: 3.528 ± 0.047	6.083AlaGly: 6.083 ± 0.067	1.406AlaHis: 1.406 ± 0.029	4.573AlaIle: 4.573 ± 0.055	3.791AlaLys: 3.791 ± 0.05	7.973AlaLeu: 7.973 ± 0.072	1.802AlaMet: 1.802 ± 0.037	3.321AlaAsn: 3.321 ± 0.051	2.913AlaPro: 2.913 ± 0.042	3.592AlaGln: 3.592 ± 0.039	3.946AlaArg: 3.946 ± 0.056	4.766AlaSer: 4.766 ± 0.063	4.694AlaThr: 4.694 ± 0.054	5.086AlaVal: 5.086 ± 0.056	1.088AlaTrp: 1.088 ± 0.023	3.008AlaTyr: 3.008 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	0.559CysAla: 0.559 ± 0.02	0.11CysCys: 0.11 ± 0.008	0.371CysAsp: 0.371 ± 0.017	0.429CysGlu: 0.429 ± 0.019	0.365CysPhe: 0.365 ± 0.015	0.617CysGly: 0.617 ± 0.02	0.213CysHis: 0.213 ± 0.011	0.458CysIle: 0.458 ± 0.015	0.302CysLys: 0.302 ± 0.013	0.805CysLeu: 0.805 ± 0.018	0.164CysMet: 0.164 ± 0.009	0.284CysAsn: 0.284 ± 0.012	0.351CysPro: 0.351 ± 0.016	0.299CysGln: 0.299 ± 0.014	0.395CysArg: 0.395 ± 0.016	0.472CysSer: 0.472 ± 0.017	0.469CysThr: 0.469 ± 0.019	0.471CysVal: 0.471 ± 0.015	0.114CysTrp: 0.114 ± 0.009	0.264CysTyr: 0.264 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.065AspAla: 4.065 ± 0.05	0.385AspCys: 0.385 ± 0.014	2.631AspAsp: 2.631 ± 0.042	3.355AspGlu: 3.355 ± 0.046	3.149AspPhe: 3.149 ± 0.042	4.056AspGly: 4.056 ± 0.069	1.026AspHis: 1.026 ± 0.025	3.117AspIle: 3.117 ± 0.044	2.936AspLys: 2.936 ± 0.05	5.371AspLeu: 5.371 ± 0.05	1.155AspMet: 1.155 ± 0.025	2.283AspAsn: 2.283 ± 0.037	2.583AspPro: 2.583 ± 0.039	2.054AspGln: 2.054 ± 0.032	2.997AspArg: 2.997 ± 0.044	3.154AspSer: 3.154 ± 0.049	2.799AspThr: 2.799 ± 0.04	3.357AspVal: 3.357 ± 0.046	0.933AspTrp: 0.933 ± 0.022	2.469AspTyr: 2.469 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	5.022GluAla: 5.022 ± 0.065	0.319GluCys: 0.319 ± 0.014	2.637GluAsp: 2.637 ± 0.035	3.675GluGlu: 3.675 ± 0.059	2.407GluPhe: 2.407 ± 0.036	3.98GluGly: 3.98 ± 0.054	1.024GluHis: 1.024 ± 0.024	3.928GluIle: 3.928 ± 0.047	4.521GluLys: 4.521 ± 0.059	5.768GluLeu: 5.768 ± 0.066	1.549GluMet: 1.549 ± 0.034	3.031GluAsn: 3.031 ± 0.042	2.014GluPro: 2.014 ± 0.032	2.433GluGln: 2.433 ± 0.039	3.135GluArg: 3.135 ± 0.04	3.218GluSer: 3.218 ± 0.046	3.249GluThr: 3.249 ± 0.045	4.299GluVal: 4.299 ± 0.049	0.809GluTrp: 0.809 ± 0.022	2.193GluTyr: 2.193 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.313PheAla: 3.313 ± 0.048	0.422PheCys: 0.422 ± 0.014	2.918PheAsp: 2.918 ± 0.038	2.765PheGlu: 2.765 ± 0.038	2.275PhePhe: 2.275 ± 0.037	3.486PheGly: 3.486 ± 0.048	0.811PheHis: 0.811 ± 0.022	2.635PheIle: 2.635 ± 0.049	2.215PheLys: 2.215 ± 0.04	4.558PheLeu: 4.558 ± 0.06	1.038PheMet: 1.038 ± 0.026	2.146PheAsn: 2.146 ± 0.035	1.888PhePro: 1.888 ± 0.038	1.676PheGln: 1.676 ± 0.029	2.426PheArg: 2.426 ± 0.034	3.396PheSer: 3.396 ± 0.048	2.941PheThr: 2.941 ± 0.041	3.118PheVal: 3.118 ± 0.041	0.666PheTrp: 0.666 ± 0.021	1.823PheTyr: 1.823 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	4.856GlyAla: 4.856 ± 0.059	0.69GlyCys: 0.69 ± 0.023	3.547GlyAsp: 3.547 ± 0.05	3.745GlyGlu: 3.745 ± 0.044	3.509GlyPhe: 3.509 ± 0.041	5.532GlyGly: 5.532 ± 0.096	1.334GlyHis: 1.334 ± 0.027	4.868GlyIle: 4.868 ± 0.05	4.726GlyLys: 4.726 ± 0.06	6.931GlyLeu: 6.931 ± 0.07	1.809GlyMet: 1.809 ± 0.031	3.367GlyAsn: 3.367 ± 0.054	2.027GlyPro: 2.027 ± 0.036	2.847GlyGln: 2.847 ± 0.037	3.562GlyArg: 3.562 ± 0.04	4.489GlySer: 4.489 ± 0.065	5.357GlyThr: 5.357 ± 0.069	4.941GlyVal: 4.941 ± 0.056	1.174GlyTrp: 1.174 ± 0.026	3.268GlyTyr: 3.268 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.332HisAla: 1.332 ± 0.03	0.181HisCys: 0.181 ± 0.011	0.978HisAsp: 0.978 ± 0.024	1.096HisGlu: 1.096 ± 0.023	1.138HisPhe: 1.138 ± 0.028	1.202HisGly: 1.202 ± 0.027	0.523HisHis: 0.523 ± 0.019	1.102HisIle: 1.102 ± 0.024	0.793HisLys: 0.793 ± 0.022	1.925HisLeu: 1.925 ± 0.036	0.34HisMet: 0.34 ± 0.013	0.757HisAsn: 0.757 ± 0.02	1.136HisPro: 1.136 ± 0.027	0.795HisGln: 0.795 ± 0.02	1.028HisArg: 1.028 ± 0.025	1.108HisSer: 1.108 ± 0.026	1.094HisThr: 1.094 ± 0.026	1.104HisVal: 1.104 ± 0.028	0.321HisTrp: 0.321 ± 0.013	0.926HisTyr: 0.926 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	4.815IleAla: 4.815 ± 0.053	0.5IleCys: 0.5 ± 0.015	3.301IleAsp: 3.301 ± 0.042	3.688IleGlu: 3.688 ± 0.047	2.498IlePhe: 2.498 ± 0.038	4.423IleGly: 4.423 ± 0.057	1.188IleHis: 1.188 ± 0.026	3.372IleIle: 3.372 ± 0.047	3.185IleLys: 3.185 ± 0.044	5.531IleLeu: 5.531 ± 0.07	1.147IleMet: 1.147 ± 0.025	2.846IleAsn: 2.846 ± 0.048	2.979IlePro: 2.979 ± 0.042	2.318IleGln: 2.318 ± 0.037	3.516IleArg: 3.516 ± 0.044	4.009IleSer: 4.009 ± 0.051	3.639IleThr: 3.639 ± 0.045	3.913IleVal: 3.913 ± 0.054	0.658IleTrp: 0.658 ± 0.018	1.978IleTyr: 1.978 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.647LysAla: 4.647 ± 0.053	0.236LysCys: 0.236 ± 0.011	2.99LysAsp: 2.99 ± 0.04	3.63LysGlu: 3.63 ± 0.056	1.854LysPhe: 1.854 ± 0.036	3.862LysGly: 3.862 ± 0.052	0.886LysHis: 0.886 ± 0.02	3.611LysIle: 3.611 ± 0.046	3.983LysLys: 3.983 ± 0.057	4.895LysLeu: 4.895 ± 0.062	1.471LysMet: 1.471 ± 0.032	2.977LysAsn: 2.977 ± 0.045	2.56LysPro: 2.56 ± 0.04	1.988LysGln: 1.988 ± 0.031	2.571LysArg: 2.571 ± 0.046	3.344LysSer: 3.344 ± 0.048	3.375LysThr: 3.375 ± 0.041	3.837LysVal: 3.837 ± 0.049	0.675LysTrp: 0.675 ± 0.021	2.164LysTyr: 2.164 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	8.457LeuAla: 8.457 ± 0.079	0.793LeuCys: 0.793 ± 0.021	5.407LeuAsp: 5.407 ± 0.059	5.262LeuGlu: 5.262 ± 0.063	4.616LeuPhe: 4.616 ± 0.053	6.867LeuGly: 6.867 ± 0.061	1.881LeuHis: 1.881 ± 0.037	5.747LeuIle: 5.747 ± 0.063	5.507LeuLys: 5.507 ± 0.055	10.512LeuLeu: 10.512 ± 0.11	2.26LeuMet: 2.26 ± 0.03	4.66LeuAsn: 4.66 ± 0.054	4.905LeuPro: 4.905 ± 0.051	3.338LeuGln: 3.338 ± 0.042	5.194LeuArg: 5.194 ± 0.055	6.904LeuSer: 6.904 ± 0.07	6.428LeuThr: 6.428 ± 0.065	6.461LeuVal: 6.461 ± 0.057	1.194LeuTrp: 1.194 ± 0.028	3.467LeuTyr: 3.467 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.999MetAla: 1.999 ± 0.035	0.124MetCys: 0.124 ± 0.008	1.183MetAsp: 1.183 ± 0.024	1.359MetGlu: 1.359 ± 0.027	0.679MetPhe: 0.679 ± 0.02	1.669MetGly: 1.669 ± 0.028	0.438MetHis: 0.438 ± 0.016	1.2MetIle: 1.2 ± 0.028	1.891MetLys: 1.891 ± 0.033	2.147MetLeu: 2.147 ± 0.036	0.551MetMet: 0.551 ± 0.017	1.2MetAsn: 1.2 ± 0.027	1.118MetPro: 1.118 ± 0.026	0.936MetGln: 0.936 ± 0.022	1.133MetArg: 1.133 ± 0.026	1.224MetSer: 1.224 ± 0.028	1.22MetThr: 1.22 ± 0.027	1.421MetVal: 1.421 ± 0.031	0.211MetTrp: 0.211 ± 0.011	0.66MetTyr: 0.66 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.559AsnAla: 3.559 ± 0.05	0.309AsnCys: 0.309 ± 0.015	2.448AsnAsp: 2.448 ± 0.038	2.532AsnGlu: 2.532 ± 0.04	2.211AsnPhe: 2.211 ± 0.039	3.583AsnGly: 3.583 ± 0.06	0.875AsnHis: 0.875 ± 0.021	2.563AsnIle: 2.563 ± 0.038	2.146AsnLys: 2.146 ± 0.035	4.515AsnLeu: 4.515 ± 0.058	0.918AsnMet: 0.918 ± 0.021	2.16AsnAsn: 2.16 ± 0.043	2.907AsnPro: 2.907 ± 0.044	2.028AsnGln: 2.028 ± 0.038	2.581AsnArg: 2.581 ± 0.036	2.814AsnSer: 2.814 ± 0.046	2.76AsnThr: 2.76 ± 0.045	2.775AsnVal: 2.775 ± 0.045	0.697AsnTrp: 0.697 ± 0.018	2.078AsnTyr: 2.078 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	3.879ProAla: 3.879 ± 0.052	0.252ProCys: 0.252 ± 0.011	3.209ProAsp: 3.209 ± 0.046	3.321ProGlu: 3.321 ± 0.042	2.071ProPhe: 2.071 ± 0.033	3.271ProGly: 3.271 ± 0.044	0.88ProHis: 0.88 ± 0.021	2.535ProIle: 2.535 ± 0.039	2.112ProLys: 2.112 ± 0.037	4.081ProLeu: 4.081 ± 0.05	0.934ProMet: 0.934 ± 0.019	2.126ProAsn: 2.126 ± 0.041	1.592ProPro: 1.592 ± 0.038	1.639ProGln: 1.639 ± 0.03	1.8ProArg: 1.8 ± 0.037	2.57ProSer: 2.57 ± 0.039	2.934ProThr: 2.934 ± 0.039	3.302ProVal: 3.302 ± 0.043	0.542ProTrp: 0.542 ± 0.017	1.799ProTyr: 1.799 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.209GlnAla: 3.209 ± 0.05	0.213GlnCys: 0.213 ± 0.011	1.749GlnAsp: 1.749 ± 0.032	2.461GlnGlu: 2.461 ± 0.036	1.674GlnPhe: 1.674 ± 0.031	2.627GlnGly: 2.627 ± 0.039	0.754GlnHis: 0.754 ± 0.021	2.381GlnIle: 2.381 ± 0.038	2.678GlnLys: 2.678 ± 0.042	4.026GlnLeu: 4.026 ± 0.05	0.926GlnMet: 0.926 ± 0.02	2.001GlnAsn: 2.001 ± 0.032	1.853GlnPro: 1.853 ± 0.031	1.928GlnGln: 1.928 ± 0.038	2.132GlnArg: 2.132 ± 0.036	2.31GlnSer: 2.31 ± 0.039	2.275GlnThr: 2.275 ± 0.035	2.627GlnVal: 2.627 ± 0.036	0.56GlnTrp: 0.56 ± 0.017	1.512GlnTyr: 1.512 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	3.534ArgAla: 3.534 ± 0.05	0.31ArgCys: 0.31 ± 0.015	2.662ArgAsp: 2.662 ± 0.042	3.192ArgGlu: 3.192 ± 0.043	2.556ArgPhe: 2.556 ± 0.04	2.92ArgGly: 2.92 ± 0.049	1.019ArgHis: 1.019 ± 0.027	3.555ArgIle: 3.555 ± 0.038	2.995ArgLys: 2.995 ± 0.039	5.172ArgLeu: 5.172 ± 0.062	1.338ArgMet: 1.338 ± 0.027	2.533ArgAsn: 2.533 ± 0.038	2.138ArgPro: 2.138 ± 0.038	2.433ArgGln: 2.433 ± 0.04	2.711ArgArg: 2.711 ± 0.042	2.911ArgSer: 2.911 ± 0.042	2.992ArgThr: 2.992 ± 0.042	3.376ArgVal: 3.376 ± 0.038	0.812ArgTrp: 0.812 ± 0.021	2.482ArgTyr: 2.482 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.515SerAla: 4.515 ± 0.055	0.534SerCys: 0.534 ± 0.017	3.241SerAsp: 3.241 ± 0.042	3.453SerGlu: 3.453 ± 0.045	3.22SerPhe: 3.22 ± 0.049	4.935SerGly: 4.935 ± 0.077	1.135SerHis: 1.135 ± 0.029	3.74SerIle: 3.74 ± 0.058	2.908SerLys: 2.908 ± 0.044	6.528SerLeu: 6.528 ± 0.057	1.292SerMet: 1.292 ± 0.027	2.593SerAsn: 2.593 ± 0.051	2.904SerPro: 2.904 ± 0.045	2.361SerGln: 2.361 ± 0.035	3.09SerArg: 3.09 ± 0.044	3.888SerSer: 3.888 ± 0.06	3.699SerThr: 3.699 ± 0.049	4.34SerVal: 4.34 ± 0.052	0.855SerTrp: 0.855 ± 0.022	2.519SerTyr: 2.519 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.701ThrAla: 4.701 ± 0.059	0.37ThrCys: 0.37 ± 0.014	3.547ThrAsp: 3.547 ± 0.048	3.273ThrGlu: 3.273 ± 0.045	2.939ThrPhe: 2.939 ± 0.039	4.682ThrGly: 4.682 ± 0.056	1.191ThrHis: 1.191 ± 0.026	3.634ThrIle: 3.634 ± 0.049	2.608ThrLys: 2.608 ± 0.035	6.587ThrLeu: 6.587 ± 0.063	1.082ThrMet: 1.082 ± 0.023	2.619ThrAsn: 2.619 ± 0.041	3.205ThrPro: 3.205 ± 0.044	2.268ThrGln: 2.268 ± 0.039	2.67ThrArg: 2.67 ± 0.034	3.611ThrSer: 3.611 ± 0.048	3.68ThrThr: 3.68 ± 0.061	4.252ThrVal: 4.252 ± 0.065	0.809ThrTrp: 0.809 ± 0.024	2.685ThrTyr: 2.685 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.426ValAla: 5.426 ± 0.064	0.591ValCys: 0.591 ± 0.019	3.417ValAsp: 3.417 ± 0.045	3.977ValGlu: 3.977 ± 0.042	3.037ValPhe: 3.037 ± 0.039	4.858ValGly: 4.858 ± 0.055	1.152ValHis: 1.152 ± 0.025	3.745ValIle: 3.745 ± 0.05	3.572ValLys: 3.572 ± 0.049	6.74ValLeu: 6.74 ± 0.067	1.48ValMet: 1.48 ± 0.03	3.049ValAsn: 3.049 ± 0.041	3.401ValPro: 3.401 ± 0.043	2.417ValGln: 2.417 ± 0.036	3.564ValArg: 3.564 ± 0.039	4.456ValSer: 4.456 ± 0.054	3.75ValThr: 3.75 ± 0.052	4.923ValVal: 4.923 ± 0.053	0.881ValTrp: 0.881 ± 0.021	2.415ValTyr: 2.415 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.952TrpAla: 0.952 ± 0.025	0.112TrpCys: 0.112 ± 0.007	0.748TrpAsp: 0.748 ± 0.021	0.779TrpGlu: 0.779 ± 0.024	0.606TrpPhe: 0.606 ± 0.021	0.98TrpGly: 0.98 ± 0.023	0.322TrpHis: 0.322 ± 0.013	0.783TrpIle: 0.783 ± 0.02	0.828TrpLys: 0.828 ± 0.022	1.484TrpLeu: 1.484 ± 0.032	0.398TrpMet: 0.398 ± 0.015	0.726TrpAsn: 0.726 ± 0.021	0.452TrpPro: 0.452 ± 0.017	0.719TrpGln: 0.719 ± 0.02	0.716TrpArg: 0.716 ± 0.019	0.722TrpSer: 0.722 ± 0.02	0.762TrpThr: 0.762 ± 0.021	0.917TrpVal: 0.917 ± 0.025	0.248TrpTrp: 0.248 ± 0.012	0.553TrpTyr: 0.553 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.953TyrAla: 2.953 ± 0.039	0.343TyrCys: 0.343 ± 0.016	2.326TyrAsp: 2.326 ± 0.041	2.279TyrGlu: 2.279 ± 0.036	2.186TyrPhe: 2.186 ± 0.036	2.945TyrGly: 2.945 ± 0.043	0.82TyrHis: 0.82 ± 0.022	1.959TyrIle: 1.959 ± 0.032	1.858TyrLys: 1.858 ± 0.037	4.309TyrLeu: 4.309 ± 0.049	0.707TyrMet: 0.707 ± 0.02	1.78TyrAsn: 1.78 ± 0.035	1.922TyrPro: 1.922 ± 0.035	1.827TyrGln: 1.827 ± 0.031	2.475TyrArg: 2.475 ± 0.04	2.384TyrSer: 2.384 ± 0.037	2.296TyrThr: 2.296 ± 0.04	2.334TyrVal: 2.334 ± 0.034	0.562TyrTrp: 0.562 ± 0.016	1.737TyrTyr: 1.737 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5629 proteins (1951742 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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