Podoviridae sp. ctDWo9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAS3|A0A5Q2WAS3_9CAUD Tail fiber protein OS=Podoviridae sp. ctDWo9 OX=2656708 PE=4 SV=1
MM1 pKa = 7.46AHH3 pKa = 6.83FAHH6 pKa = 7.19ISNGQVSQVIVISNDD21 pKa = 3.12DD22 pKa = 3.64CGGGDD27 pKa = 4.03FPQSEE32 pKa = 4.56PVGQAFIASLWLSGEE47 pKa = 3.97WRR49 pKa = 11.84QTSYY53 pKa = 11.26NANFRR58 pKa = 11.84GKK60 pKa = 10.07YY61 pKa = 10.04AGIGDD66 pKa = 4.46TYY68 pKa = 11.37DD69 pKa = 3.45ADD71 pKa = 4.03ADD73 pKa = 4.15EE74 pKa = 4.97FTSPPIAEE82 pKa = 4.12PTAA85 pKa = 3.76

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W8J7|A0A5Q2W8J7_9CAUD Uncharacterized protein OS=Podoviridae sp. ctDWo9 OX=2656708 PE=4 SV=1
MM1 pKa = 7.27GGVRR5 pKa = 11.84GGQRR9 pKa = 11.84NLPLRR14 pKa = 11.84RR15 pKa = 11.84PSRR18 pKa = 11.84RR19 pKa = 11.84GKK21 pKa = 8.57PARR24 pKa = 11.84RR25 pKa = 11.84TRR27 pKa = 11.84NALRR31 pKa = 11.84NHH33 pKa = 6.54LWW35 pKa = 3.19

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11048

34

3154

263.0

28.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.88 ± 0.971

0.824 ± 0.274

5.829 ± 0.246

6.363 ± 0.491

3.231 ± 0.211

8.599 ± 0.847

1.828 ± 0.218

3.919 ± 0.192

5.051 ± 0.512

8.02 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.2

4.091 ± 0.285

4.127 ± 0.329

3.376 ± 0.301

7.024 ± 0.399

6.897 ± 0.432

5.965 ± 0.393

6.445 ± 0.292

1.693 ± 0.306

3.249 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski