Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Amphibacillus; Amphibacillus xylanus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0J5Y7|K0J5Y7_AMPXN Histidine kinase OS=Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01) OX=698758 GN=AXY_23790 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.37KK3 pKa = 10.28RR4 pKa = 11.84ILAILLVLIFALVACGDD21 pKa = 4.11KK22 pKa = 10.44DD23 pKa = 3.73TASPNDD29 pKa = 3.47VEE31 pKa = 6.69GDD33 pKa = 3.57DD34 pKa = 3.97TDD36 pKa = 3.87NTEE39 pKa = 3.81EE40 pKa = 4.12TTDD43 pKa = 3.45EE44 pKa = 4.3VEE46 pKa = 4.27EE47 pKa = 4.25EE48 pKa = 4.57EE49 pKa = 4.31PTVDD53 pKa = 4.09YY54 pKa = 10.71EE55 pKa = 4.28QTPEE59 pKa = 3.72MDD61 pKa = 3.35FDD63 pKa = 4.49LGGRR67 pKa = 11.84TLKK70 pKa = 10.71LVSWYY75 pKa = 10.09DD76 pKa = 3.56EE77 pKa = 4.15QVGEE81 pKa = 4.5GSPDD85 pKa = 3.51SIAISEE91 pKa = 4.08NMEE94 pKa = 4.1ALKK97 pKa = 10.45EE98 pKa = 3.91KK99 pKa = 10.82HH100 pKa = 6.33NFDD103 pKa = 3.12IEE105 pKa = 4.65YY106 pKa = 10.59VVVDD110 pKa = 3.17YY111 pKa = 11.59GEE113 pKa = 4.01YY114 pKa = 9.72QEE116 pKa = 5.85RR117 pKa = 11.84VTASLIAGEE126 pKa = 4.09PLGDD130 pKa = 4.21IIRR133 pKa = 11.84LPRR136 pKa = 11.84PWMIPTLTRR145 pKa = 11.84QGLFHH150 pKa = 7.11PVDD153 pKa = 3.47EE154 pKa = 4.47YY155 pKa = 11.83VINEE159 pKa = 3.87NSFVLQYY166 pKa = 10.36TEE168 pKa = 3.75QHH170 pKa = 5.97SEE172 pKa = 3.68FDD174 pKa = 3.19GRR176 pKa = 11.84GYY178 pKa = 10.8GFRR181 pKa = 11.84VGIAGAAGGVFYY193 pKa = 11.15NRR195 pKa = 11.84TLMNEE200 pKa = 4.08LNLDD204 pKa = 3.72PLQAYY209 pKa = 9.1VDD211 pKa = 3.54NGEE214 pKa = 4.4WNWEE218 pKa = 4.03TFKK221 pKa = 11.28AVAEE225 pKa = 4.36SANQDD230 pKa = 3.54TNNDD234 pKa = 3.29GTIDD238 pKa = 3.38TWGLATSSILVQALAANEE256 pKa = 3.74AAIVRR261 pKa = 11.84NGKK264 pKa = 9.52QNLEE268 pKa = 4.11DD269 pKa = 3.93PATIEE274 pKa = 3.75VMEE277 pKa = 5.45FISEE281 pKa = 4.19LGDD284 pKa = 3.87GIARR288 pKa = 11.84PTEE291 pKa = 4.38GGDD294 pKa = 2.9WTEE297 pKa = 3.86PKK299 pKa = 10.58QFFLQGNTLMYY310 pKa = 10.3VGQDD314 pKa = 3.22YY315 pKa = 11.42EE316 pKa = 4.15MADD319 pKa = 4.54FIDD322 pKa = 4.54GLPDD326 pKa = 3.41HH327 pKa = 7.69DD328 pKa = 4.88IGFLPFPMGPSASSYY343 pKa = 6.71QTHH346 pKa = 5.39ITIPNYY352 pKa = 8.74YY353 pKa = 8.8TIPSAVEE360 pKa = 4.02DD361 pKa = 4.09PDD363 pKa = 5.0KK364 pKa = 10.9IVYY367 pKa = 9.19LWEE370 pKa = 3.86KK371 pKa = 9.7MYY373 pKa = 10.55DD374 pKa = 3.05IEE376 pKa = 5.84SIYY379 pKa = 10.51DD380 pKa = 3.53YY381 pKa = 10.98PEE383 pKa = 3.52QANFEE388 pKa = 4.47THH390 pKa = 6.65FNNEE394 pKa = 3.72DD395 pKa = 4.14DD396 pKa = 4.0INNARR401 pKa = 11.84LAVQSFQVIEE411 pKa = 4.18QIDD414 pKa = 4.78YY415 pKa = 8.97YY416 pKa = 10.52PSMPYY421 pKa = 10.36YY422 pKa = 9.8EE423 pKa = 4.76FSGEE427 pKa = 3.96LSDD430 pKa = 4.83GVNISTLIEE439 pKa = 4.22SYY441 pKa = 11.1GPSFQAAIDD450 pKa = 4.16EE451 pKa = 4.64VWGEE455 pKa = 3.9

Molecular weight: 51.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0IUW5|K0IUW5_AMPXN Beta sliding clamp OS=Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01) OX=698758 GN=dnaN PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.36NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.01KK14 pKa = 7.87VHH16 pKa = 5.71GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.02NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.51KK37 pKa = 10.32GRR39 pKa = 11.84RR40 pKa = 11.84VLSAA44 pKa = 3.76

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2384

0

2384

713264

26

3128

299.2

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.537 ± 0.049

0.568 ± 0.014

5.86 ± 0.048

7.504 ± 0.061

4.359 ± 0.041

6.254 ± 0.052

2.099 ± 0.024

8.524 ± 0.052

6.513 ± 0.052

9.746 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.021

4.779 ± 0.039

3.419 ± 0.035

4.401 ± 0.047

4.234 ± 0.038

5.759 ± 0.03

5.498 ± 0.038

6.728 ± 0.038

0.911 ± 0.02

3.833 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski