Amino acid dipepetide frequency for Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.22AlaAla: 4.22 ± 0.103
0.461AlaCys: 0.461 ± 0.027
3.791AlaAsp: 3.791 ± 0.085
4.822AlaGlu: 4.822 ± 0.097
2.709AlaPhe: 2.709 ± 0.065
4.482AlaGly: 4.482 ± 0.096
1.245AlaHis: 1.245 ± 0.04
6.12AlaIle: 6.12 ± 0.109
4.669AlaLys: 4.669 ± 0.095
6.532AlaLeu: 6.532 ± 0.103
1.659AlaMet: 1.659 ± 0.048
3.103AlaAsn: 3.103 ± 0.082
1.691AlaPro: 1.691 ± 0.054
2.449AlaGln: 2.449 ± 0.068
2.72AlaArg: 2.72 ± 0.057
3.648AlaSer: 3.648 ± 0.071
3.54AlaThr: 3.54 ± 0.07
4.523AlaVal: 4.523 ± 0.09
0.562AlaTrp: 0.562 ± 0.034
2.43AlaTyr: 2.43 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.329CysAla: 0.329 ± 0.024
0.062CysCys: 0.062 ± 0.01
0.322CysAsp: 0.322 ± 0.026
0.376CysGlu: 0.376 ± 0.027
0.254CysPhe: 0.254 ± 0.019
0.512CysGly: 0.512 ± 0.027
0.191CysHis: 0.191 ± 0.017
0.4CysIle: 0.4 ± 0.023
0.255CysLys: 0.255 ± 0.02
0.578CysLeu: 0.578 ± 0.033
0.129CysMet: 0.129 ± 0.013
0.212CysAsn: 0.212 ± 0.02
0.297CysPro: 0.297 ± 0.021
0.296CysGln: 0.296 ± 0.021
0.245CysArg: 0.245 ± 0.022
0.37CysSer: 0.37 ± 0.021
0.255CysThr: 0.255 ± 0.019
0.324CysVal: 0.324 ± 0.022
0.036CysTrp: 0.036 ± 0.007
0.24CysTyr: 0.24 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.103AspAla: 3.103 ± 0.071
0.351AspCys: 0.351 ± 0.023
3.215AspAsp: 3.215 ± 0.088
4.871AspGlu: 4.871 ± 0.091
2.741AspPhe: 2.741 ± 0.065
3.53AspGly: 3.53 ± 0.087
1.583AspHis: 1.583 ± 0.051
4.502AspIle: 4.502 ± 0.087
3.298AspLys: 3.298 ± 0.091
5.959AspLeu: 5.959 ± 0.101
1.286AspMet: 1.286 ± 0.044
2.36AspAsn: 2.36 ± 0.07
2.318AspPro: 2.318 ± 0.069
4.021AspGln: 4.021 ± 0.084
2.863AspArg: 2.863 ± 0.06
2.674AspSer: 2.674 ± 0.071
2.584AspThr: 2.584 ± 0.063
3.877AspVal: 3.877 ± 0.075
0.733AspTrp: 0.733 ± 0.035
2.735AspTyr: 2.735 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
5.504GluAla: 5.504 ± 0.094
0.273GluCys: 0.273 ± 0.019
4.062GluAsp: 4.062 ± 0.083
6.34GluGlu: 6.34 ± 0.128
2.507GluPhe: 2.507 ± 0.053
3.703GluGly: 3.703 ± 0.09
1.445GluHis: 1.445 ± 0.047
6.268GluIle: 6.268 ± 0.099
5.674GluLys: 5.674 ± 0.096
7.656GluLeu: 7.656 ± 0.115
2.132GluMet: 2.132 ± 0.061
3.553GluAsn: 3.553 ± 0.083
2.177GluPro: 2.177 ± 0.061
4.224GluGln: 4.224 ± 0.087
3.606GluArg: 3.606 ± 0.091
3.655GluSer: 3.655 ± 0.075
4.014GluThr: 4.014 ± 0.083
5.186GluVal: 5.186 ± 0.114
0.767GluTrp: 0.767 ± 0.031
2.291GluTyr: 2.291 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.683PheAla: 2.683 ± 0.062
0.244PheCys: 0.244 ± 0.019
2.773PheAsp: 2.773 ± 0.064
2.995PheGlu: 2.995 ± 0.062
2.069PhePhe: 2.069 ± 0.065
3.047PheGly: 3.047 ± 0.079
0.77PheHis: 0.77 ± 0.035
3.966PheIle: 3.966 ± 0.083
2.752PheLys: 2.752 ± 0.064
3.857PheLeu: 3.857 ± 0.087
0.955PheMet: 0.955 ± 0.038
2.332PheAsn: 2.332 ± 0.064
1.445PhePro: 1.445 ± 0.049
1.448PheGln: 1.448 ± 0.046
1.56PheArg: 1.56 ± 0.048
3.065PheSer: 3.065 ± 0.068
2.441PheThr: 2.441 ± 0.061
2.953PheVal: 2.953 ± 0.063
0.467PheTrp: 0.467 ± 0.025
1.767PheTyr: 1.767 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
4.099GlyAla: 4.099 ± 0.083
0.471GlyCys: 0.471 ± 0.031
3.307GlyAsp: 3.307 ± 0.077
4.346GlyGlu: 4.346 ± 0.09
3.021GlyPhe: 3.021 ± 0.074
4.032GlyGly: 4.032 ± 0.116
1.37GlyHis: 1.37 ± 0.049
5.396GlyIle: 5.396 ± 0.106
4.308GlyLys: 4.308 ± 0.088
6.144GlyLeu: 6.144 ± 0.106
1.769GlyMet: 1.769 ± 0.052
2.477GlyAsn: 2.477 ± 0.061
1.724GlyPro: 1.724 ± 0.221
2.296GlyGln: 2.296 ± 0.063
2.681GlyArg: 2.681 ± 0.062
3.513GlySer: 3.513 ± 0.077
3.54GlyThr: 3.54 ± 0.08
4.638GlyVal: 4.638 ± 0.092
0.613GlyTrp: 0.613 ± 0.035
2.789GlyTyr: 2.789 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.273HisAla: 1.273 ± 0.041
0.17HisCys: 0.17 ± 0.015
1.256HisAsp: 1.256 ± 0.04
1.391HisGlu: 1.391 ± 0.047
1.05HisPhe: 1.05 ± 0.039
1.343HisGly: 1.343 ± 0.047
0.642HisHis: 0.642 ± 0.03
1.593HisIle: 1.593 ± 0.05
1.023HisLys: 1.023 ± 0.043
2.233HisLeu: 2.233 ± 0.058
0.404HisMet: 0.404 ± 0.022
0.859HisAsn: 0.859 ± 0.037
1.081HisPro: 1.081 ± 0.047
1.052HisGln: 1.052 ± 0.041
0.873HisArg: 0.873 ± 0.033
1.054HisSer: 1.054 ± 0.038
1.002HisThr: 1.002 ± 0.043
1.426HisVal: 1.426 ± 0.045
0.206HisTrp: 0.206 ± 0.018
1.061HisTyr: 1.061 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
6.001IleAla: 6.001 ± 0.103
0.531IleCys: 0.531 ± 0.028
5.939IleAsp: 5.939 ± 0.081
7.195IleGlu: 7.195 ± 0.123
3.543IlePhe: 3.543 ± 0.086
5.818IleGly: 5.818 ± 0.106
1.73IleHis: 1.73 ± 0.055
6.899IleIle: 6.899 ± 0.113
5.476IleLys: 5.476 ± 0.099
7.177IleLeu: 7.177 ± 0.123
1.73IleMet: 1.73 ± 0.05
4.516IleAsn: 4.516 ± 0.081
3.236IlePro: 3.236 ± 0.065
3.005IleGln: 3.005 ± 0.067
3.234IleArg: 3.234 ± 0.065
5.29IleSer: 5.29 ± 0.094
4.744IleThr: 4.744 ± 0.088
5.647IleVal: 5.647 ± 0.088
0.658IleTrp: 0.658 ± 0.027
2.876IleTyr: 2.876 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
4.392LysAla: 4.392 ± 0.083
0.294LysCys: 0.294 ± 0.022
3.739LysAsp: 3.739 ± 0.078
5.49LysGlu: 5.49 ± 0.105
1.956LysPhe: 1.956 ± 0.05
3.621LysGly: 3.621 ± 0.075
1.353LysHis: 1.353 ± 0.044
4.878LysIle: 4.878 ± 0.085
4.831LysLys: 4.831 ± 0.108
6.03LysLeu: 6.03 ± 0.103
1.883LysMet: 1.883 ± 0.052
3.089LysAsn: 3.089 ± 0.073
2.083LysPro: 2.083 ± 0.053
3.872LysGln: 3.872 ± 0.081
3.335LysArg: 3.335 ± 0.073
3.467LysSer: 3.467 ± 0.077
3.464LysThr: 3.464 ± 0.078
4.45LysVal: 4.45 ± 0.082
0.627LysTrp: 0.627 ± 0.03
2.277LysTyr: 2.277 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
7.157LeuAla: 7.157 ± 0.108
0.482LeuCys: 0.482 ± 0.029
5.706LeuAsp: 5.706 ± 0.097
6.821LeuGlu: 6.821 ± 0.103
4.477LeuPhe: 4.477 ± 0.105
5.663LeuGly: 5.663 ± 0.103
1.638LeuHis: 1.638 ± 0.05
8.851LeuIle: 8.851 ± 0.133
6.501LeuLys: 6.501 ± 0.109
9.461LeuLeu: 9.461 ± 0.169
2.35LeuMet: 2.35 ± 0.065
5.161LeuAsn: 5.161 ± 0.096
3.593LeuPro: 3.593 ± 0.073
3.034LeuGln: 3.034 ± 0.069
3.642LeuArg: 3.642 ± 0.074
6.746LeuSer: 6.746 ± 0.103
6.149LeuThr: 6.149 ± 0.092
6.179LeuVal: 6.179 ± 0.11
0.649LeuTrp: 0.649 ± 0.031
3.198LeuTyr: 3.198 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
1.734MetAla: 1.734 ± 0.047
0.105MetCys: 0.105 ± 0.012
1.265MetAsp: 1.265 ± 0.043
1.566MetGlu: 1.566 ± 0.046
0.882MetPhe: 0.882 ± 0.036
1.416MetGly: 1.416 ± 0.047
0.412MetHis: 0.412 ± 0.023
2.35MetIle: 2.35 ± 0.062
2.026MetLys: 2.026 ± 0.053
2.311MetLeu: 2.311 ± 0.062
0.76MetMet: 0.76 ± 0.031
1.447MetAsn: 1.447 ± 0.043
0.886MetPro: 0.886 ± 0.037
0.838MetGln: 0.838 ± 0.034
1.157MetArg: 1.157 ± 0.046
1.569MetSer: 1.569 ± 0.053
1.631MetThr: 1.631 ± 0.049
1.527MetVal: 1.527 ± 0.044
0.156MetTrp: 0.156 ± 0.016
0.7MetTyr: 0.7 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.696AsnAla: 2.696 ± 0.066
0.29AsnCys: 0.29 ± 0.021
2.78AsnAsp: 2.78 ± 0.075
3.738AsnGlu: 3.738 ± 0.078
1.844AsnPhe: 1.844 ± 0.051
3.093AsnGly: 3.093 ± 0.079
1.27AsnHis: 1.27 ± 0.044
3.529AsnIle: 3.529 ± 0.077
3.091AsnLys: 3.091 ± 0.062
4.433AsnLeu: 4.433 ± 0.086
1.255AsnMet: 1.255 ± 0.039
2.308AsnAsn: 2.308 ± 0.065
2.081AsnPro: 2.081 ± 0.053
3.241AsnGln: 3.241 ± 0.088
2.441AsnArg: 2.441 ± 0.061
2.184AsnSer: 2.184 ± 0.057
2.022AsnThr: 2.022 ± 0.053
2.852AsnVal: 2.852 ± 0.069
0.625AsnTrp: 0.625 ± 0.032
2.019AsnTyr: 2.019 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
1.932ProAla: 1.932 ± 0.054
0.195ProCys: 0.195 ± 0.019
2.148ProAsp: 2.148 ± 0.059
2.916ProGlu: 2.916 ± 0.077
1.712ProPhe: 1.712 ± 0.048
2.117ProGly: 2.117 ± 0.069
0.73ProHis: 0.73 ± 0.031
3.226ProIle: 3.226 ± 0.07
2.123ProLys: 2.123 ± 0.057
3.149ProLeu: 3.149 ± 0.07
0.732ProMet: 0.732 ± 0.035
1.991ProAsn: 1.991 ± 0.053
0.758ProPro: 0.758 ± 0.038
1.0ProGln: 1.0 ± 0.041
1.077ProArg: 1.077 ± 0.045
2.032ProSer: 2.032 ± 0.053
2.218ProThr: 2.218 ± 0.22
2.466ProVal: 2.466 ± 0.059
0.268ProTrp: 0.268 ± 0.02
1.402ProTyr: 1.402 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
3.502GlnAla: 3.502 ± 0.077
0.161GlnCys: 0.161 ± 0.017
2.092GlnAsp: 2.092 ± 0.06
3.014GlnGlu: 3.014 ± 0.07
2.134GlnPhe: 2.134 ± 0.067
2.179GlnGly: 2.179 ± 0.045
0.838GlnHis: 0.838 ± 0.034
3.791GlnIle: 3.791 ± 0.077
2.549GlnLys: 2.549 ± 0.062
5.541GlnLeu: 5.541 ± 0.126
1.283GlnMet: 1.283 ± 0.044
1.738GlnAsn: 1.738 ± 0.053
1.466GlnPro: 1.466 ± 0.05
2.064GlnGln: 2.064 ± 0.068
1.64GlnArg: 1.64 ± 0.054
2.629GlnSer: 2.629 ± 0.07
2.423GlnThr: 2.423 ± 0.062
2.867GlnVal: 2.867 ± 0.065
0.421GlnTrp: 0.421 ± 0.027
1.674GlnTyr: 1.674 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
2.543ArgAla: 2.543 ± 0.064
0.2ArgCys: 0.2 ± 0.015
2.334ArgAsp: 2.334 ± 0.055
3.145ArgGlu: 3.145 ± 0.072
1.967ArgPhe: 1.967 ± 0.059
2.427ArgGly: 2.427 ± 0.066
0.901ArgHis: 0.901 ± 0.032
3.414ArgIle: 3.414 ± 0.065
3.063ArgLys: 3.063 ± 0.082
4.436ArgLeu: 4.436 ± 0.081
1.171ArgMet: 1.171 ± 0.04
1.94ArgAsn: 1.94 ± 0.044
1.426ArgPro: 1.426 ± 0.047
1.889ArgGln: 1.889 ± 0.056
1.98ArgArg: 1.98 ± 0.047
2.287ArgSer: 2.287 ± 0.061
2.266ArgThr: 2.266 ± 0.058
2.786ArgVal: 2.786 ± 0.064
0.356ArgTrp: 0.356 ± 0.023
1.807ArgTyr: 1.807 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
3.37SerAla: 3.37 ± 0.068
0.301SerCys: 0.301 ± 0.02
3.319SerAsp: 3.319 ± 0.083
3.996SerGlu: 3.996 ± 0.085
2.895SerPhe: 2.895 ± 0.057
4.186SerGly: 4.186 ± 0.077
1.279SerHis: 1.279 ± 0.045
5.336SerIle: 5.336 ± 0.101
3.384SerLys: 3.384 ± 0.073
5.694SerLeu: 5.694 ± 0.096
1.448SerMet: 1.448 ± 0.048
2.636SerAsn: 2.636 ± 0.069
1.771SerPro: 1.771 ± 0.056
2.197SerGln: 2.197 ± 0.059
2.344SerArg: 2.344 ± 0.05
3.441SerSer: 3.441 ± 0.089
3.052SerThr: 3.052 ± 0.058
3.99SerVal: 3.99 ± 0.074
0.522SerTrp: 0.522 ± 0.029
2.431SerTyr: 2.431 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.585ThrAla: 3.585 ± 0.069
0.282ThrCys: 0.282 ± 0.019
3.424ThrAsp: 3.424 ± 0.074
3.898ThrGlu: 3.898 ± 0.085
2.546ThrPhe: 2.546 ± 0.062
4.083ThrGly: 4.083 ± 0.238
1.105ThrHis: 1.105 ± 0.04
5.165ThrIle: 5.165 ± 0.085
3.345ThrLys: 3.345 ± 0.064
5.068ThrLeu: 5.068 ± 0.08
1.195ThrMet: 1.195 ± 0.038
2.66ThrAsn: 2.66 ± 0.064
2.137ThrPro: 2.137 ± 0.055
1.765ThrGln: 1.765 ± 0.051
1.971ThrArg: 1.971 ± 0.046
2.989ThrSer: 2.989 ± 0.07
3.108ThrThr: 3.108 ± 0.066
4.123ThrVal: 4.123 ± 0.083
0.47ThrTrp: 0.47 ± 0.028
2.067ThrTyr: 2.067 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
4.669ValAla: 4.669 ± 0.08
0.443ValCys: 0.443 ± 0.023
4.317ValAsp: 4.317 ± 0.096
5.133ValGlu: 5.133 ± 0.122
2.831ValPhe: 2.831 ± 0.067
4.37ValGly: 4.37 ± 0.096
1.203ValHis: 1.203 ± 0.04
6.113ValIle: 6.113 ± 0.104
4.442ValLys: 4.442 ± 0.076
6.017ValLeu: 6.017 ± 0.112
1.618ValMet: 1.618 ± 0.047
3.265ValAsn: 3.265 ± 0.072
2.254ValPro: 2.254 ± 0.062
2.09ValGln: 2.09 ± 0.051
2.567ValArg: 2.567 ± 0.058
4.273ValSer: 4.273 ± 0.071
4.064ValThr: 4.064 ± 0.074
4.761ValVal: 4.761 ± 0.099
0.491ValTrp: 0.491 ± 0.026
2.36ValTyr: 2.36 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.46TrpAla: 0.46 ± 0.03
0.07TrpCys: 0.07 ± 0.01
0.543TrpAsp: 0.543 ± 0.031
0.572TrpGlu: 0.572 ± 0.033
0.456TrpPhe: 0.456 ± 0.027
0.53TrpGly: 0.53 ± 0.028
0.184TrpHis: 0.184 ± 0.016
0.728TrpIle: 0.728 ± 0.033
0.527TrpLys: 0.527 ± 0.03
1.108TrpLeu: 1.108 ± 0.036
0.247TrpMet: 0.247 ± 0.017
0.433TrpAsn: 0.433 ± 0.027
0.245TrpPro: 0.245 ± 0.021
0.486TrpGln: 0.486 ± 0.028
0.426TrpArg: 0.426 ± 0.023
0.566TrpSer: 0.566 ± 0.028
0.501TrpThr: 0.501 ± 0.027
0.536TrpVal: 0.536 ± 0.028
0.121TrpTrp: 0.121 ± 0.013
0.369TrpTyr: 0.369 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.12TyrAla: 2.12 ± 0.052
0.294TyrCys: 0.294 ± 0.021
2.268TyrAsp: 2.268 ± 0.068
2.416TyrGlu: 2.416 ± 0.06
1.996TyrPhe: 1.996 ± 0.058
2.385TyrGly: 2.385 ± 0.056
1.105TyrHis: 1.105 ± 0.044
2.721TyrIle: 2.721 ± 0.067
1.795TyrLys: 1.795 ± 0.049
4.076TyrLeu: 4.076 ± 0.091
0.73TyrMet: 0.73 ± 0.032
1.713TyrAsn: 1.713 ± 0.06
1.52TyrPro: 1.52 ± 0.052
2.738TyrGln: 2.738 ± 0.069
1.974TyrArg: 1.974 ± 0.05
2.139TyrSer: 2.139 ± 0.053
1.966TyrThr: 1.966 ± 0.064
2.173TyrVal: 2.173 ± 0.057
0.36TyrTrp: 0.36 ± 0.023
1.837TyrTyr: 1.837 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2384 proteins (713264 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski