Rotavirus B (isolate RVB/Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus B

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6NGG0|D6NGG0_ROTGA Nonstructural protein NSP1-1 OS=Rotavirus B (isolate RVB/Human/China/ADRV/1982) OX=10942 PE=4 SV=1
MM1 pKa = 7.78ASLPLLVLAAAVNAQLNIVPSVHH24 pKa = 6.89PEE26 pKa = 3.86VCVLYY31 pKa = 10.78ADD33 pKa = 5.01DD34 pKa = 4.87HH35 pKa = 7.2QSDD38 pKa = 3.83ANKK41 pKa = 10.23FNGNFTQIFHH51 pKa = 7.04SYY53 pKa = 11.01NSITLSFMSYY63 pKa = 10.24SSSSYY68 pKa = 11.12DD69 pKa = 3.35VIDD72 pKa = 3.17IMSRR76 pKa = 11.84YY77 pKa = 9.48DD78 pKa = 3.86LSSCSILAIDD88 pKa = 4.74VFDD91 pKa = 4.98ASMDD95 pKa = 3.83FNVFLQSTNNCSKK108 pKa = 10.78YY109 pKa = 9.86NANKK113 pKa = 9.08IHH115 pKa = 6.37YY116 pKa = 8.63IKK118 pKa = 10.57LPRR121 pKa = 11.84GEE123 pKa = 4.29EE124 pKa = 3.76WFSYY128 pKa = 10.32SKK130 pKa = 10.84NLKK133 pKa = 9.63FCPLSDD139 pKa = 3.85SLIGMYY145 pKa = 10.34CDD147 pKa = 3.57TQLSDD152 pKa = 3.69TYY154 pKa = 11.31FEE156 pKa = 4.61ISTGGTYY163 pKa = 10.29EE164 pKa = 4.04VTDD167 pKa = 3.7VPEE170 pKa = 4.39FTQMGYY176 pKa = 7.7TFHH179 pKa = 8.12SSEE182 pKa = 5.04DD183 pKa = 3.61FYY185 pKa = 11.53LCHH188 pKa = 7.18RR189 pKa = 11.84ISSEE193 pKa = 3.11AWLNYY198 pKa = 9.38HH199 pKa = 6.99LFYY202 pKa = 10.62RR203 pKa = 11.84DD204 pKa = 3.31YY205 pKa = 10.9DD206 pKa = 3.83VSGVISKK213 pKa = 9.89QINWGNVWSGFKK225 pKa = 10.1TFAQVLYY232 pKa = 10.46KK233 pKa = 10.41ILDD236 pKa = 3.49LFFNSKK242 pKa = 10.65RR243 pKa = 11.84NVEE246 pKa = 4.02PRR248 pKa = 11.84AA249 pKa = 3.51

Molecular weight: 28.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6NGG1|D6NGG1_ROTGA Nonstructural protein NSP1-2 OS=Rotavirus B (isolate RVB/Human/China/ADRV/1982) OX=10942 PE=4 SV=1
MM1 pKa = 7.84GNRR4 pKa = 11.84QSSAQLNSHH13 pKa = 6.25LTHH16 pKa = 7.03INSQNSNLFISDD28 pKa = 3.04SKK30 pKa = 9.88TAVFHH35 pKa = 5.6TQHH38 pKa = 6.61ILLAAGVGIIATLLVLLLCSCVLNCYY64 pKa = 10.22LCRR67 pKa = 11.84KK68 pKa = 9.37LKK70 pKa = 9.25RR71 pKa = 11.84TNGVSSLLEE80 pKa = 3.64RR81 pKa = 11.84NIRR84 pKa = 11.84QNGSSAKK91 pKa = 9.89IYY93 pKa = 9.41VKK95 pKa = 10.32PVMQSSTIIEE105 pKa = 4.17EE106 pKa = 4.13AA107 pKa = 3.36

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5712

107

1160

476.0

54.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.777 ± 0.388

1.366 ± 0.238

6.548 ± 0.306

5.41 ± 0.756

4.482 ± 0.454

3.957 ± 0.505

2.223 ± 0.219

7.668 ± 0.326

6.285 ± 0.506

8.841 ± 0.588

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.271

6.057 ± 0.445

3.589 ± 0.355

4.132 ± 0.271

5.182 ± 0.387

8.298 ± 0.486

6.46 ± 0.29

5.83 ± 0.422

0.963 ± 0.124

4.114 ± 0.484
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski