Pigmentiphaga humi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pigmentiphaga

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P4AVG1|A0A3P4AVG1_9BURK S-adenosylmethionine synthase OS=Pigmentiphaga humi OX=2478468 GN=metK PE=3 SV=1
MM1 pKa = 7.62LPEE4 pKa = 5.08AGTVPAVAEE13 pKa = 4.07LLLLLGAALVDD24 pKa = 4.01TVPVEE29 pKa = 4.1ALVPAVSLEE38 pKa = 4.18SGVALAALDD47 pKa = 4.2AAPPLPPPPPTDD59 pKa = 3.59CANTPAEE66 pKa = 4.28FAPCVAIVPVLVTATVPAFPPPPPLPPRR94 pKa = 11.84PNEE97 pKa = 3.9AAALPPASAVLLAAPPVPPPPPIDD121 pKa = 3.86CANTPSDD128 pKa = 4.2CVPVVAMLPLLTTEE142 pKa = 4.71TAPPSPPTAPDD153 pKa = 3.62PPRR156 pKa = 11.84LTEE159 pKa = 4.99AEE161 pKa = 4.39LLPPVAFEE169 pKa = 4.4LLEE172 pKa = 4.6PPLPPPPPTDD182 pKa = 3.6CANTPLDD189 pKa = 3.82RR190 pKa = 11.84SPVVLIAATVATLTVLPLPPSPPAPPMLSDD220 pKa = 3.93APMPLSAPDD229 pKa = 3.28WFAGVLLLEE238 pKa = 4.6NFCAADD244 pKa = 4.08TVALPAVLPTKK255 pKa = 10.3PVV257 pKa = 2.9

Molecular weight: 25.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P4AYG1|A0A3P4AYG1_9BURK Haloalkane dehalogenase OS=Pigmentiphaga humi OX=2478468 GN=dhaA_1 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.24TRR25 pKa = 11.84AGRR28 pKa = 11.84AIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4682

0

4682

1541785

30

7768

329.3

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.173 ± 0.054

0.932 ± 0.012

5.425 ± 0.033

5.389 ± 0.034

3.337 ± 0.02

8.585 ± 0.037

2.253 ± 0.018

4.349 ± 0.025

2.757 ± 0.029

10.546 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.02

2.397 ± 0.02

5.64 ± 0.027

3.706 ± 0.02

7.615 ± 0.048

5.311 ± 0.027

4.849 ± 0.056

7.504 ± 0.03

1.411 ± 0.015

2.381 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski