Porphyromonas endodontalis (strain ATCC 35406 / BCRC 14492 / JCM 8526 / NCTC 13058 / HG 370)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1965 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3J9E9|C3J9E9_POREA PAP2 family protein OS=Porphyromonas endodontalis (strain ATCC 35406 / BCRC 14492 / JCM 8526 / NCTC 13058 / HG 370) OX=553175 GN=POREN0001_1093 PE=4 SV=1
MM1 pKa = 7.63 EE2 pKa = 4.62 ATDD5 pKa = 4.17 LNEE8 pKa = 3.23 ARR10 pKa = 11.84 IYY12 pKa = 10.4 VGTYY16 pKa = 9.66 AKK18 pKa = 10.48 YY19 pKa = 11.08 NNGSLQGEE27 pKa = 4.35 WVEE30 pKa = 5.59 LSDD33 pKa = 6.54 FYY35 pKa = 11.8 DD36 pKa = 3.73 LDD38 pKa = 3.98 GFMEE42 pKa = 4.67 RR43 pKa = 11.84 CAEE46 pKa = 3.73 IHH48 pKa = 6.44 EE49 pKa = 4.91 DD50 pKa = 3.87 EE51 pKa = 4.66 EE52 pKa = 5.21 APEE55 pKa = 3.96 YY56 pKa = 10.31 MFQAWEE62 pKa = 4.46 EE63 pKa = 4.28 IPDD66 pKa = 3.75 GLIDD70 pKa = 4.0 EE71 pKa = 4.67 GHH73 pKa = 6.61 LEE75 pKa = 3.97 EE76 pKa = 5.04 TFFEE80 pKa = 5.11 LRR82 pKa = 11.84 DD83 pKa = 3.54 EE84 pKa = 4.95 LDD86 pKa = 3.37 RR87 pKa = 11.84 LNDD90 pKa = 3.65 KK91 pKa = 10.7 EE92 pKa = 5.76 KK93 pKa = 10.68 GAFWVWADD101 pKa = 3.79 GNSSQLTQDD110 pKa = 3.22 AYY112 pKa = 11.76 SLVKK116 pKa = 10.47 SFQSDD121 pKa = 4.36 YY122 pKa = 10.36 IGSYY126 pKa = 10.73 ASRR129 pKa = 11.84 EE130 pKa = 4.07 DD131 pKa = 3.69 FAEE134 pKa = 4.24 EE135 pKa = 3.68 LAKK138 pKa = 10.14 MEE140 pKa = 4.32 NALSDD145 pKa = 3.48 FALNYY150 pKa = 10.23 FDD152 pKa = 3.98 FSKK155 pKa = 11.2 YY156 pKa = 10.79 ADD158 pKa = 4.7 DD159 pKa = 5.56 LFDD162 pKa = 3.53 MDD164 pKa = 4.02 FWYY167 pKa = 11.21 NDD169 pKa = 3.24 GYY171 pKa = 10.84 VFRR174 pKa = 11.84 NNN176 pKa = 3.2
Molecular weight: 20.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.923
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|C3JA08|C3JA08_POREA Uncharacterized protein OS=Porphyromonas endodontalis (strain ATCC 35406 / BCRC 14492 / JCM 8526 / NCTC 13058 / HG 370) OX=553175 GN=POREN0001_0930 PE=4 SV=1
MM1 pKa = 7.34 GVYY4 pKa = 9.91 EE5 pKa = 5.27 SPDD8 pKa = 3.44 SLLKK12 pKa = 9.82 EE13 pKa = 4.0 HH14 pKa = 6.47 RR15 pKa = 11.84 QEE17 pKa = 3.46 ISGIRR22 pKa = 11.84 KK23 pKa = 8.6 LAYY26 pKa = 9.18 RR27 pKa = 11.84 HH28 pKa = 6.25 LGRR31 pKa = 11.84 HH32 pKa = 5.73 LMNCNVKK39 pKa = 10.27 KK40 pKa = 10.03 IRR42 pKa = 11.84 NRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 AKK48 pKa = 10.55 DD49 pKa = 3.03 II50 pKa = 3.92
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.78
IPC_protein 10.599
Toseland 10.847
ProMoST 10.555
Dawson 10.921
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.169
Grimsley 10.965
Solomon 11.067
Lehninger 11.038
Nozaki 10.833
DTASelect 10.628
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 10.95
IPC_peptide 11.082
IPC2_peptide 9.619
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1965
0
1965
619246
35
2494
315.1
35.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.806 ± 0.056
1.105 ± 0.021
5.042 ± 0.043
7.031 ± 0.063
4.209 ± 0.03
6.891 ± 0.046
2.132 ± 0.022
6.436 ± 0.049
5.717 ± 0.059
10.255 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.024
3.989 ± 0.041
4.308 ± 0.033
3.626 ± 0.035
5.533 ± 0.045
6.692 ± 0.042
5.52 ± 0.042
6.36 ± 0.045
1.068 ± 0.02
3.887 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here