Veillonella sp. oral taxon 780 str. F0422
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1585 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9N5Z9|F9N5Z9_9FIRM Uncharacterized protein OS=Veillonella sp. oral taxon 780 str. F0422 OX=944564 GN=HMPREF9200_1244 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 9.96 EE3 pKa = 4.05 KK4 pKa = 10.66 KK5 pKa = 9.98 DD6 pKa = 3.46 VSNYY10 pKa = 9.7 YY11 pKa = 10.79 SNSEE15 pKa = 4.24 LLHH18 pKa = 6.62 DD19 pKa = 3.91 QDD21 pKa = 4.76 FPFTIEE27 pKa = 3.88 EE28 pKa = 4.17 ADD30 pKa = 3.8 NAILDD35 pKa = 3.98 YY36 pKa = 10.67 MPEE39 pKa = 4.33 LLNEE43 pKa = 4.71 DD44 pKa = 4.05 YY45 pKa = 11.24 QPDD48 pKa = 3.69 AEE50 pKa = 4.23
Molecular weight: 5.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|F9N6L5|F9N6L5_9FIRM DNA replication and repair protein RecF OS=Veillonella sp. oral taxon 780 str. F0422 OX=944564 GN=recF PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPNTLWRR12 pKa = 11.84 KK13 pKa = 6.72 RR14 pKa = 11.84 THH16 pKa = 6.25 GFRR19 pKa = 11.84 EE20 pKa = 3.78 RR21 pKa = 11.84 MKK23 pKa = 10.34 TIGGRR28 pKa = 11.84 LVLKK32 pKa = 10.36 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.164
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.901
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1585
0
1585
495418
33
2577
312.6
34.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.993 ± 0.09
0.987 ± 0.025
5.381 ± 0.053
6.961 ± 0.081
3.621 ± 0.05
7.75 ± 0.07
2.333 ± 0.033
7.142 ± 0.072
5.883 ± 0.065
9.054 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.996 ± 0.036
3.93 ± 0.07
3.737 ± 0.038
3.669 ± 0.048
4.462 ± 0.054
5.59 ± 0.052
6.114 ± 0.082
7.935 ± 0.064
0.926 ± 0.024
3.537 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here