Ferrithrix thermotolerans DSM 19514

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; Acidimicrobiales; Acidimicrobiaceae; Ferrithrix; Ferrithrix thermotolerans

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4WJM3|A0A1M4WJM3_9ACTN DNA polymerase-3 subunit epsilon OS=Ferrithrix thermotolerans DSM 19514 OX=1121881 GN=SAMN02745225_01695 PE=4 SV=1
MM1 pKa = 7.07TRR3 pKa = 11.84SLSAAISGIDD13 pKa = 4.66ASQAWLDD20 pKa = 3.83NIGNNIANVDD30 pKa = 3.74TVGYY34 pKa = 9.89QSTDD38 pKa = 2.67IQFADD43 pKa = 4.72LLYY46 pKa = 10.38QQQQSAGAPQVGVNGGTNPIEE67 pKa = 4.34IGSGVRR73 pKa = 11.84VASTGINFTQGTLSQTGVQTDD94 pKa = 3.95LAIQGQGFLVVQQGGNTFYY113 pKa = 10.88TRR115 pKa = 11.84AGNLQLDD122 pKa = 3.99AAGEE126 pKa = 4.22LVTPSGAIVQGWTPNAAGVINTSGPLTNLTIPQGQAANPTATKK169 pKa = 10.49NINLGGNLPAWNGSGTAPSYY189 pKa = 9.33TATVTAFDD197 pKa = 4.06SLGGQIPITLTFTASTTPNTWSVVATAPNPAGGTDD232 pKa = 3.28TLTPAGTTISFNGTTGQMTSTAPITLSGFTGYY264 pKa = 11.31NNLPAGYY271 pKa = 8.7TMNLDD276 pKa = 4.19FPAAGTPQAVTQFSGTSTIQVTSQDD301 pKa = 3.64GNASGTLDD309 pKa = 3.45GFTISSNGEE318 pKa = 3.53IVGNYY323 pKa = 10.18SNGNTQPLGQIALAQFANDD342 pKa = 3.07QGLAKK347 pKa = 10.38VGNLLYY353 pKa = 10.5QATLNSGAPQLGLAGSGGLGTLVGGAVEE381 pKa = 4.2TSNVNLGTQLTDD393 pKa = 4.77LIVAQTAYY401 pKa = 10.23QANTKK406 pKa = 9.63VVSTTSTVLQSLVQMAA422 pKa = 4.2

Molecular weight: 42.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4XH81|A0A1M4XH81_9ACTN Fur family transcriptional regulator ferric uptake regulator OS=Ferrithrix thermotolerans DSM 19514 OX=1121881 GN=SAMN02745225_02021 PE=3 SV=1
MM1 pKa = 7.26NVHH4 pKa = 6.65LVDD7 pKa = 3.6QAAFDD12 pKa = 4.4AVDD15 pKa = 3.15TCVRR19 pKa = 11.84HH20 pKa = 5.73LASIVATSSLKK31 pKa = 9.95AKK33 pKa = 9.58IWRR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 7.69PNGAEE43 pKa = 3.29RR44 pKa = 11.84HH45 pKa = 4.49YY46 pKa = 11.16AYY48 pKa = 10.58ACLEE52 pKa = 4.01RR53 pKa = 11.84SSALGIIMRR62 pKa = 11.84GGTPDD67 pKa = 2.4ITMRR71 pKa = 11.84QHH73 pKa = 6.87SLTKK77 pKa = 10.12KK78 pKa = 7.99EE79 pKa = 3.96RR80 pKa = 11.84KK81 pKa = 9.61AIASYY86 pKa = 9.15LHH88 pKa = 6.19RR89 pKa = 11.84ALRR92 pKa = 11.84KK93 pKa = 9.61ALAHH97 pKa = 5.81TWPTVRR103 pKa = 11.84LVRR106 pKa = 11.84SMALDD111 pKa = 3.36EE112 pKa = 4.3TLYY115 pKa = 10.94SSFVIEE121 pKa = 4.48RR122 pKa = 11.84PGKK125 pKa = 7.78EE126 pKa = 4.22HH127 pKa = 7.36PGKK130 pKa = 8.66TPKK133 pKa = 9.85RR134 pKa = 11.84RR135 pKa = 11.84QYY137 pKa = 10.2VQVIGPRR144 pKa = 11.84PRR146 pKa = 11.84EE147 pKa = 4.19RR148 pKa = 11.84ITIPLSGVSRR158 pKa = 11.84VSGNIRR164 pKa = 11.84VVLDD168 pKa = 3.73EE169 pKa = 4.38GSTRR173 pKa = 11.84AFIHH177 pKa = 5.43VAYY180 pKa = 9.52EE181 pKa = 3.96IQPLPGPATGPDD193 pKa = 3.3VAIDD197 pKa = 3.24WGITEE202 pKa = 4.2VCTDD206 pKa = 3.77DD207 pKa = 5.46SGIKK211 pKa = 10.13HH212 pKa = 5.76GASYY216 pKa = 10.9GAILKK221 pKa = 10.27RR222 pKa = 11.84ATEE225 pKa = 3.85QRR227 pKa = 11.84KK228 pKa = 7.88RR229 pKa = 11.84TGQVRR234 pKa = 11.84NKK236 pKa = 9.66LWAITKK242 pKa = 9.88KK243 pKa = 10.67DD244 pKa = 3.59AGSKK248 pKa = 8.17RR249 pKa = 11.84AKK251 pKa = 10.33RR252 pKa = 11.84IAKK255 pKa = 9.94YY256 pKa = 10.79NLGTKK261 pKa = 9.06KK262 pKa = 9.51QARR265 pKa = 11.84RR266 pKa = 11.84RR267 pKa = 11.84ARR269 pKa = 11.84TQAALQTVAGAAIKK283 pKa = 9.97EE284 pKa = 4.28VIYY287 pKa = 11.18GEE289 pKa = 4.33GNRR292 pKa = 11.84TRR294 pKa = 11.84ARR296 pKa = 11.84GKK298 pKa = 9.88VPQLPAHH305 pKa = 6.8RR306 pKa = 11.84PRR308 pKa = 11.84RR309 pKa = 11.84VIVEE313 pKa = 4.41DD314 pKa = 3.88LAQLRR319 pKa = 11.84GKK321 pKa = 10.2AKK323 pKa = 9.83SKK325 pKa = 10.49KK326 pKa = 9.46ISRR329 pKa = 11.84LCSSWARR336 pKa = 11.84SEE338 pKa = 4.05NEE340 pKa = 3.1EE341 pKa = 3.97RR342 pKa = 11.84MAVHH346 pKa = 7.17AYY348 pKa = 9.43IGRR351 pKa = 11.84SDD353 pKa = 3.61VRR355 pKa = 11.84VVSAAYY361 pKa = 8.85TSQTCPDD368 pKa = 3.31PHH370 pKa = 7.87YY371 pKa = 10.96GYY373 pKa = 10.95VSQDD377 pKa = 2.96NRR379 pKa = 11.84SGDD382 pKa = 3.26WFHH385 pKa = 6.98CRR387 pKa = 11.84NPYY390 pKa = 8.79WEE392 pKa = 5.02CNRR395 pKa = 11.84QGDD398 pKa = 4.32ADD400 pKa = 3.99HH401 pKa = 6.49VAAVNLKK408 pKa = 8.76TRR410 pKa = 11.84INDD413 pKa = 3.72PEE415 pKa = 3.87IHH417 pKa = 6.97RR418 pKa = 11.84FTPHH422 pKa = 7.43IEE424 pKa = 3.94VKK426 pKa = 10.49KK427 pKa = 10.54ILDD430 pKa = 3.27ARR432 pKa = 11.84FVCRR436 pKa = 11.84RR437 pKa = 11.84EE438 pKa = 3.88SRR440 pKa = 11.84TKK442 pKa = 10.97GGDD445 pKa = 3.04ASPASRR451 pKa = 11.84GQRR454 pKa = 11.84DD455 pKa = 3.45ATSTSGAQGASVDD468 pKa = 4.12GAATAHH474 pKa = 6.03GRR476 pKa = 11.84TSIPRR481 pKa = 11.84RR482 pKa = 11.84SDD484 pKa = 3.19PDD486 pKa = 3.43VGGGTTTDD494 pKa = 3.03LHH496 pKa = 6.2RR497 pKa = 11.84VRR499 pKa = 11.84SIWRR503 pKa = 11.84GPGRR507 pKa = 11.84LSSARR512 pKa = 11.84AKK514 pKa = 9.58TKK516 pKa = 10.2GVPSVPLQKK525 pKa = 9.86FTEE528 pKa = 4.31KK529 pKa = 10.38QQ530 pKa = 3.06

Molecular weight: 58.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2339

0

2339

706337

10

1597

302.0

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.016 ± 0.049

0.843 ± 0.017

5.313 ± 0.045

6.482 ± 0.056

3.742 ± 0.029

7.808 ± 0.05

1.929 ± 0.021

5.304 ± 0.034

4.177 ± 0.031

10.572 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.017

2.802 ± 0.03

4.54 ± 0.036

3.092 ± 0.038

6.142 ± 0.051

8.03 ± 0.057

5.395 ± 0.043

8.737 ± 0.051

1.11 ± 0.021

2.863 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski