Malvastrum yellow vein virus-[Y47]
Average proteome isoelectric point is 7.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QKZ7|Q8QKZ7_9GEMI Protein V2 OS=Malvastrum yellow vein virus-[Y47] OX=222476 GN=AV2 PE=3 SV=1
MM1 pKa = 7.99 WDD3 pKa = 3.38 PLVNEE8 pKa = 4.72 FPEE11 pKa = 4.61 TVHH14 pKa = 6.81 GFRR17 pKa = 11.84 CMISIKK23 pKa = 10.21 YY24 pKa = 9.32 LQLLSEE30 pKa = 5.34 GYY32 pKa = 10.46 SPDD35 pKa = 3.25 TVGYY39 pKa = 10.39 DD40 pKa = 4.42 LIRR43 pKa = 11.84 EE44 pKa = 4.71 LISILRR50 pKa = 11.84 SRR52 pKa = 11.84 NYY54 pKa = 9.91 VEE56 pKa = 4.26 ASCRR60 pKa = 11.84 YY61 pKa = 7.53 RR62 pKa = 11.84 HH63 pKa = 6.36 FYY65 pKa = 10.07 PRR67 pKa = 11.84 VEE69 pKa = 4.24 GASSSEE75 pKa = 3.66 LRR77 pKa = 11.84 QPLLNPCNCPHH88 pKa = 7.01 CPRR91 pKa = 11.84 HH92 pKa = 6.15 KK93 pKa = 9.94 ISNVGEE99 pKa = 4.24 QAHH102 pKa = 6.22 EE103 pKa = 4.0 PQTQNVQDD111 pKa = 3.9 VQKK114 pKa = 11.07 SS115 pKa = 3.37
Molecular weight: 13.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.997
IPC2_protein 6.084
IPC_protein 6.211
Toseland 6.415
ProMoST 6.532
Dawson 6.576
Bjellqvist 6.504
Wikipedia 6.59
Rodwell 6.561
Grimsley 6.517
Solomon 6.576
Lehninger 6.576
Nozaki 6.825
DTASelect 7.0
Thurlkill 7.029
EMBOSS 7.015
Sillero 6.971
Patrickios 3.427
IPC_peptide 6.59
IPC2_peptide 6.912
IPC2.peptide.svr19 6.793
Protein with the highest isoelectric point:
>tr|Q8QKZ7|Q8QKZ7_9GEMI Protein V2 OS=Malvastrum yellow vein virus-[Y47] OX=222476 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.52 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.06 SPYY27 pKa = 7.79 STRR30 pKa = 11.84 VTVPTVRR37 pKa = 11.84 ATKK40 pKa = 10.64 SRR42 pKa = 11.84 MWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.67 VTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.09 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.39 EE124 pKa = 4.08 NIKK127 pKa = 10.76 SKK129 pKa = 10.6 NHH131 pKa = 5.54 TNNVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.88 RR143 pKa = 11.84 RR144 pKa = 11.84 PTGTPQDD151 pKa = 3.8 FGDD154 pKa = 3.96 VFNMFDD160 pKa = 4.34 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.07 RR173 pKa = 11.84 DD174 pKa = 3.2 RR175 pKa = 11.84 YY176 pKa = 8.85 QVVRR180 pKa = 11.84 KK181 pKa = 9.26 WYY183 pKa = 8.07 ATVTGGQYY191 pKa = 9.96 GAKK194 pKa = 9.62 EE195 pKa = 3.57 QALVKK200 pKa = 10.7 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.08 YY219 pKa = 8.01 EE220 pKa = 4.07 NHH222 pKa = 6.29 SEE224 pKa = 4.11 NALMLYY230 pKa = 7.58 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.575
IPC_protein 9.867
Toseland 10.145
ProMoST 9.882
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.687
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.277
Patrickios 10.204
IPC_peptide 10.379
IPC2_peptide 8.946
IPC2.peptide.svr19 8.55
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1116
99
362
186.0
21.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.108 ± 0.677
2.33 ± 0.484
4.749 ± 0.473
4.839 ± 0.649
4.391 ± 0.516
4.839 ± 0.38
3.674 ± 0.611
5.376 ± 1.008
6.183 ± 0.663
7.437 ± 1.117
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.885
5.914 ± 0.374
6.004 ± 0.5
5.018 ± 0.736
6.452 ± 1.123
8.781 ± 1.304
5.556 ± 0.916
5.556 ± 1.525
1.344 ± 0.247
3.674 ± 0.67
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here