Hubei permutotetra-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHT6|A0A1L3KHT6_9VIRU Capsid protein OS=Hubei permutotetra-like virus 8 OX=1923082 PE=3 SV=1
MM1 pKa = 7.71TDD3 pKa = 3.39CSNPIVDD10 pKa = 4.06HH11 pKa = 6.35SRR13 pKa = 11.84VPFGNLEE20 pKa = 3.86DD21 pKa = 3.75VAAVRR26 pKa = 11.84LRR28 pKa = 11.84QDD30 pKa = 3.51LGALRR35 pKa = 11.84ALLEE39 pKa = 4.11TSVRR43 pKa = 11.84EE44 pKa = 3.91TLPAPEE50 pKa = 5.18HH51 pKa = 5.85RR52 pKa = 11.84VDD54 pKa = 2.71WEE56 pKa = 4.28GRR58 pKa = 11.84VKK60 pKa = 11.13ADD62 pKa = 3.02ISKK65 pKa = 10.72LNEE68 pKa = 3.63GLKK71 pKa = 10.24IKK73 pKa = 10.73VEE75 pKa = 4.14SGVLDD80 pKa = 3.46IKK82 pKa = 11.29KK83 pKa = 8.39NTSLVEE89 pKa = 4.08YY90 pKa = 10.04SEE92 pKa = 4.68HH93 pKa = 6.48FPVSGLRR100 pKa = 11.84HH101 pKa = 6.15EE102 pKa = 4.83GQPVHH107 pKa = 6.63PAGVHH112 pKa = 4.73QKK114 pKa = 9.64EE115 pKa = 4.66GWVTTQRR122 pKa = 11.84TFGGNLRR129 pKa = 11.84RR130 pKa = 11.84IDD132 pKa = 3.67EE133 pKa = 4.28VQAFLRR139 pKa = 11.84VFEE142 pKa = 4.34PEE144 pKa = 3.95EE145 pKa = 3.84MLEE148 pKa = 4.11AATQLTYY155 pKa = 10.89TRR157 pKa = 11.84GTAKK161 pKa = 10.44GFEE164 pKa = 4.05TRR166 pKa = 11.84IEE168 pKa = 3.93KK169 pKa = 10.63LVTRR173 pKa = 11.84KK174 pKa = 9.86CKK176 pKa = 10.35SVLKK180 pKa = 10.2YY181 pKa = 10.28PYY183 pKa = 9.99VSKK186 pKa = 10.95DD187 pKa = 3.16LNSAVKK193 pKa = 10.23RR194 pKa = 11.84LRR196 pKa = 11.84EE197 pKa = 4.03LLPLRR202 pKa = 11.84KK203 pKa = 9.77GSLLPDD209 pKa = 3.06WGGTNLEE216 pKa = 4.83DD217 pKa = 4.86LLEE220 pKa = 4.75TIQTTARR227 pKa = 11.84ASAGAPYY234 pKa = 10.09WKK236 pKa = 9.93DD237 pKa = 2.9KK238 pKa = 11.18CDD240 pKa = 3.66AKK242 pKa = 10.87EE243 pKa = 3.83QLLFAGLPEE252 pKa = 4.89LVEE255 pKa = 4.49ALNKK259 pKa = 9.87EE260 pKa = 4.12DD261 pKa = 3.53PKK263 pKa = 11.38AYY265 pKa = 10.5LSLFDD270 pKa = 5.19RR271 pKa = 11.84DD272 pKa = 4.13PEE274 pKa = 4.19MFLCEE279 pKa = 4.85CKK281 pKa = 10.67NKK283 pKa = 10.03LDD285 pKa = 3.86RR286 pKa = 11.84YY287 pKa = 7.18EE288 pKa = 4.06TSKK291 pKa = 11.35LDD293 pKa = 3.85TKK295 pKa = 10.49CRR297 pKa = 11.84PYY299 pKa = 10.75FSIPYY304 pKa = 8.16HH305 pKa = 6.37CSVLFSILSQNFSDD319 pKa = 4.66NLEE322 pKa = 4.33LCHH325 pKa = 6.81EE326 pKa = 4.69GDD328 pKa = 3.5GANAYY333 pKa = 10.0GLVWAHH339 pKa = 6.87GGATKK344 pKa = 10.35LAEE347 pKa = 4.17WVDD350 pKa = 3.54KK351 pKa = 9.35RR352 pKa = 11.84TKK354 pKa = 8.01YY355 pKa = 10.23QKK357 pKa = 9.16PTFYY361 pKa = 10.6CYY363 pKa = 11.03GDD365 pKa = 3.54DD366 pKa = 3.67TDD368 pKa = 6.19FYY370 pKa = 10.96WRR372 pKa = 11.84DD373 pKa = 3.22QAGEE377 pKa = 4.09LWRR380 pKa = 11.84ASPDD384 pKa = 3.48FVQMDD389 pKa = 3.98GSVDD393 pKa = 3.66RR394 pKa = 11.84DD395 pKa = 3.82CVRR398 pKa = 11.84VAVEE402 pKa = 3.75YY403 pKa = 10.03IYY405 pKa = 10.9TSFAEE410 pKa = 3.77QHH412 pKa = 5.27GQSRR416 pKa = 11.84FWRR419 pKa = 11.84RR420 pKa = 11.84VCDD423 pKa = 3.37LWIAMATDD431 pKa = 3.96PLFIIHH437 pKa = 7.0GPTVYY442 pKa = 10.51RR443 pKa = 11.84KK444 pKa = 7.12PTRR447 pKa = 11.84DD448 pKa = 3.23GLASGVVGTTFFDD461 pKa = 3.24TCKK464 pKa = 10.57AVLSYY469 pKa = 11.37SSFLDD474 pKa = 3.14HH475 pKa = 5.52VTYY478 pKa = 11.48NNMHH482 pKa = 6.61SYY484 pKa = 10.97AFKK487 pKa = 10.86QKK489 pKa = 9.56TSDD492 pKa = 3.19KK493 pKa = 10.8FEE495 pKa = 4.15VEE497 pKa = 4.26EE498 pKa = 4.89YY499 pKa = 10.39MLDD502 pKa = 3.63SCGLEE507 pKa = 3.89IKK509 pKa = 10.54QGTWVPNKK517 pKa = 10.11VCVQPQPGNLFCDD530 pKa = 3.74SKK532 pKa = 11.43FLGVYY537 pKa = 7.7LQWRR541 pKa = 11.84QGPEE545 pKa = 3.9KK546 pKa = 11.0VEE548 pKa = 3.85LVPWLPEE555 pKa = 3.94EE556 pKa = 4.91DD557 pKa = 3.31WLKK560 pKa = 10.42TILNPRR566 pKa = 11.84DD567 pKa = 3.43LHH569 pKa = 6.49EE570 pKa = 5.73DD571 pKa = 3.62EE572 pKa = 5.38VNKK575 pKa = 10.44KK576 pKa = 8.35PQDD579 pKa = 3.34YY580 pKa = 10.58RR581 pKa = 11.84SIIRR585 pKa = 11.84QRR587 pKa = 11.84TTFDD591 pKa = 3.08RR592 pKa = 11.84ARR594 pKa = 11.84GYY596 pKa = 10.84LITGAFSNEE605 pKa = 3.72RR606 pKa = 11.84VRR608 pKa = 11.84DD609 pKa = 3.78ALFHH613 pKa = 5.91VLNSVDD619 pKa = 3.59PVAVCMEE626 pKa = 4.09VAGGGGKK633 pKa = 10.29GEE635 pKa = 4.02QPEE638 pKa = 4.18ISNGLGNFQYY648 pKa = 10.25HH649 pKa = 7.31DD650 pKa = 3.86SSGVPTQKK658 pKa = 9.56WCEE661 pKa = 3.85NLYY664 pKa = 8.73FTSTNQWQGDD674 pKa = 4.24KK675 pKa = 11.01LWLQPFGAVADD686 pKa = 4.94KK687 pKa = 10.84IGIWKK692 pKa = 10.12RR693 pKa = 11.84EE694 pKa = 3.75EE695 pKa = 3.92RR696 pKa = 11.84VVNPRR701 pKa = 11.84LTVKK705 pKa = 10.33HH706 pKa = 6.13GEE708 pKa = 3.87IEE710 pKa = 4.15LVGKK714 pKa = 10.49KK715 pKa = 9.75EE716 pKa = 3.95LVEE719 pKa = 4.1VEE721 pKa = 4.37PPPVLSAGLTQVKK734 pKa = 9.75KK735 pKa = 10.63LKK737 pKa = 10.16HH738 pKa = 5.63EE739 pKa = 4.39KK740 pKa = 9.98PVTFAEE746 pKa = 4.22ALKK749 pKa = 10.3KK750 pKa = 10.44PNVRR754 pKa = 11.84SEE756 pKa = 3.93LRR758 pKa = 11.84DD759 pKa = 3.34PVTKK763 pKa = 10.36EE764 pKa = 3.85KK765 pKa = 10.11PKK767 pKa = 10.84HH768 pKa = 6.13SGTDD772 pKa = 3.06VDD774 pKa = 6.12HH775 pKa = 6.9FLAWLNEE782 pKa = 4.07EE783 pKa = 4.24KK784 pKa = 10.74KK785 pKa = 9.75LTGSVGMPGVPLVCFRR801 pKa = 11.84NAFGLSWMKK810 pKa = 10.72AKK812 pKa = 10.34EE813 pKa = 4.1RR814 pKa = 11.84VFKK817 pKa = 10.93AGFLIQGRR825 pKa = 11.84DD826 pKa = 3.05QWIVPRR832 pKa = 11.84FVSGLDD838 pKa = 3.34EE839 pKa = 4.6EE840 pKa = 6.0GNVQKK845 pKa = 10.55QVSAAKK851 pKa = 9.18TLSEE855 pKa = 4.33VVGPLTKK862 pKa = 10.27GAKK865 pKa = 8.99QVLQKK870 pKa = 10.9VPVSNPLAAALKK882 pKa = 10.07PKK884 pKa = 10.71VEE886 pKa = 4.23VSWVEE891 pKa = 4.42DD892 pKa = 3.25IDD894 pKa = 3.94EE895 pKa = 4.48KK896 pKa = 11.59VYY898 pKa = 10.52ILKK901 pKa = 10.75GLLQASLDD909 pKa = 3.78HH910 pKa = 6.24GLKK913 pKa = 10.54GLSLSEE919 pKa = 5.01DD920 pKa = 3.23KK921 pKa = 11.25MNDD924 pKa = 2.68EE925 pKa = 4.54TFWKK929 pKa = 10.12KK930 pKa = 10.39VSHH933 pKa = 6.76LKK935 pKa = 10.77FKK937 pKa = 10.3DD938 pKa = 3.11QHH940 pKa = 5.72PFKK943 pKa = 10.66LHH945 pKa = 5.64LVDD948 pKa = 4.05RR949 pKa = 11.84SRR951 pKa = 11.84RR952 pKa = 11.84IVSKK956 pKa = 10.45EE957 pKa = 3.82GQSTLSAVFFLEE969 pKa = 3.96MRR971 pKa = 11.84HH972 pKa = 6.07KK973 pKa = 10.94NGASLDD979 pKa = 3.9FSQLGAVKK987 pKa = 10.45APLLSVCRR995 pKa = 11.84NTFYY999 pKa = 11.13HH1000 pKa = 7.43LLRR1003 pKa = 11.84EE1004 pKa = 4.17KK1005 pKa = 10.99LRR1007 pKa = 11.84GFQVEE1012 pKa = 4.2IPVPKK1017 pKa = 10.36AAEE1020 pKa = 4.17FKK1022 pKa = 10.75NDD1024 pKa = 3.18SQTTLLTALSSGVPEE1039 pKa = 4.62EE1040 pKa = 4.15NTAGDD1045 pKa = 3.91ILHH1048 pKa = 6.55GTDD1051 pKa = 3.47QTHH1054 pKa = 5.29NWGDD1058 pKa = 3.4HH1059 pKa = 5.06VEE1061 pKa = 4.2RR1062 pKa = 11.84QDD1064 pKa = 3.81VLDD1067 pKa = 3.74KK1068 pKa = 11.06SRR1070 pKa = 11.84PNIVFTGHH1078 pKa = 6.04FQQHH1082 pKa = 6.56EE1083 pKa = 4.18IDD1085 pKa = 4.99EE1086 pKa = 4.59ALAWLQVTPPPSGEE1100 pKa = 4.08GPLEE1104 pKa = 3.93VSRR1107 pKa = 11.84VKK1109 pKa = 11.1VKK1111 pKa = 10.74DD1112 pKa = 3.47KK1113 pKa = 11.02EE1114 pKa = 4.29VLLVRR1119 pKa = 11.84RR1120 pKa = 11.84VGAEE1124 pKa = 3.34IEE1126 pKa = 4.08EE1127 pKa = 4.13ARR1129 pKa = 11.84NLSYY1133 pKa = 7.86TTLTQEE1139 pKa = 4.27GLSTVVAEE1147 pKa = 4.33NPGFKK1152 pKa = 9.91SEE1154 pKa = 4.32KK1155 pKa = 10.55SPIKK1159 pKa = 10.22NLIAITHH1166 pKa = 5.67NDD1168 pKa = 2.97EE1169 pKa = 4.27RR1170 pKa = 11.84KK1171 pKa = 9.91EE1172 pKa = 3.92SSEE1175 pKa = 3.88NYY1177 pKa = 9.34RR1178 pKa = 11.84KK1179 pKa = 9.96GRR1181 pKa = 11.84KK1182 pKa = 6.58TAEE1185 pKa = 3.97QRR1187 pKa = 11.84HH1188 pKa = 5.03KK1189 pKa = 10.77WNVRR1193 pKa = 11.84DD1194 pKa = 3.93GKK1196 pKa = 10.16RR1197 pKa = 11.84KK1198 pKa = 9.52KK1199 pKa = 9.86RR1200 pKa = 11.84RR1201 pKa = 11.84KK1202 pKa = 9.41LEE1204 pKa = 3.85KK1205 pKa = 9.97KK1206 pKa = 9.08QRR1208 pKa = 11.84QEE1210 pKa = 3.9EE1211 pKa = 4.84GEE1213 pKa = 4.13KK1214 pKa = 10.52EE1215 pKa = 4.19AII1217 pKa = 3.85

Molecular weight: 138.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHY9|A0A1L3KHY9_9VIRU Uncharacterized protein OS=Hubei permutotetra-like virus 8 OX=1923082 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 4.69CARR5 pKa = 11.84WKK7 pKa = 7.26TQKK10 pKa = 10.55EE11 pKa = 3.8EE12 pKa = 4.05EE13 pKa = 4.24ARR15 pKa = 11.84KK16 pKa = 8.37EE17 pKa = 4.06TTPGGRR23 pKa = 11.84RR24 pKa = 11.84EE25 pKa = 3.9RR26 pKa = 11.84SNLEE30 pKa = 3.29WLARR34 pKa = 11.84PGHH37 pKa = 6.61WLTPLLPEE45 pKa = 4.57GNRR48 pKa = 11.84LGPLLRR54 pKa = 11.84SHH56 pKa = 7.0KK57 pKa = 10.02GYY59 pKa = 11.4VVMTDD64 pKa = 3.01CCLYY68 pKa = 10.93LILLNMVQGRR78 pKa = 11.84SLLRR82 pKa = 11.84FPLCPNCVQDD92 pKa = 4.19LLKK95 pKa = 10.74RR96 pKa = 11.84LALGRR101 pKa = 11.84EE102 pKa = 4.38LNTEE106 pKa = 3.99SLGFPLSLKK115 pKa = 10.83LEE117 pKa = 4.27LQVPVAPSWLLSKK130 pKa = 8.66TQQTRR135 pKa = 11.84FLKK138 pKa = 10.87GKK140 pKa = 7.81TDD142 pKa = 3.76YY143 pKa = 11.45NVFLHH148 pKa = 6.96KK149 pKa = 9.93IRR151 pKa = 11.84RR152 pKa = 11.84LLHH155 pKa = 6.45LFGNPPTLRR164 pKa = 11.84WLGSEE169 pKa = 4.32TYY171 pKa = 8.59ITPTSMQMSPGGLHH185 pKa = 7.19LGQFVLL191 pKa = 4.96

Molecular weight: 21.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1821

191

1217

607.0

68.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.26 ± 1.085

1.867 ± 0.329

5.162 ± 0.816

7.414 ± 0.778

3.789 ± 0.343

7.194 ± 0.402

2.197 ± 0.64

2.91 ± 0.275

7.908 ± 0.627

10.544 ± 1.77

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.318 ± 0.38

3.734 ± 0.24

5.382 ± 0.472

4.064 ± 0.067

6.15 ± 0.454

6.37 ± 0.664

5.546 ± 0.285

7.688 ± 0.869

1.977 ± 0.272

2.526 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski