Mycobacterium phage NothingSpecial

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KSG7|A0A516KSG7_9CAUD DnaB-like dsDNA helicase OS=Mycobacterium phage NothingSpecial OX=2590945 GN=65 PE=4 SV=1
MM1 pKa = 7.36NPDD4 pKa = 3.74DD5 pKa = 5.81LYY7 pKa = 10.98TFAIRR12 pKa = 11.84YY13 pKa = 7.6EE14 pKa = 4.32GQQPDD19 pKa = 4.52GEE21 pKa = 4.34WGEE24 pKa = 4.25MVAIQPNLAQAHH36 pKa = 5.7AAWDD40 pKa = 3.56EE41 pKa = 3.95MAPYY45 pKa = 11.01LEE47 pKa = 4.52MFPNVRR53 pKa = 11.84NAGIVSTPKK62 pKa = 9.28ITWQPYY68 pKa = 7.03TADD71 pKa = 3.56IGTDD75 pKa = 3.36PTDD78 pKa = 2.98

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KSG4|A0A516KSG4_9CAUD Terminase OS=Mycobacterium phage NothingSpecial OX=2590945 GN=14 PE=4 SV=1
MM1 pKa = 7.66TSTKK5 pKa = 10.47LPYY8 pKa = 9.42LHH10 pKa = 6.99KK11 pKa = 10.04NARR14 pKa = 11.84SRR16 pKa = 11.84RR17 pKa = 11.84ITSKK21 pKa = 10.47EE22 pKa = 3.33IRR24 pKa = 11.84EE25 pKa = 4.19VFAEE29 pKa = 4.12EE30 pKa = 4.03VTRR33 pKa = 11.84NLPRR37 pKa = 11.84GLDD40 pKa = 3.26RR41 pKa = 11.84EE42 pKa = 4.24EE43 pKa = 4.2YY44 pKa = 9.73LRR46 pKa = 11.84KK47 pKa = 10.25VMPP50 pKa = 4.57

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

16196

33

843

184.0

20.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.243 ± 0.386

0.827 ± 0.111

6.366 ± 0.201

6.724 ± 0.314

3.371 ± 0.174

8.169 ± 0.488

1.982 ± 0.189

4.952 ± 0.219

4.822 ± 0.258

8.243 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.116

3.149 ± 0.214

5.711 ± 0.273

3.556 ± 0.205

6.304 ± 0.365

5.248 ± 0.227

5.884 ± 0.215

7.039 ± 0.228

1.858 ± 0.139

3.044 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski