Leptolyngbya sp. hensonii
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8ZH54|A0A1Q8ZH54_9CYAN Uncharacterized protein OS=Leptolyngbya sp. 'hensonii' OX=1922337 GN=BST81_05125 PE=4 SV=1
MM1 pKa = 7.68 DD2 pKa = 3.93 VEE4 pKa = 4.64 VDD6 pKa = 3.68 VQICVAGSGADD17 pKa = 3.55 GCPEE21 pKa = 3.85 SGAWQAYY28 pKa = 5.43 FQQWLEE34 pKa = 3.97 LLLPTEE40 pKa = 5.02 APNQVYY46 pKa = 10.58 LDD48 pKa = 3.66 WEE50 pKa = 3.94 QDD52 pKa = 3.48 YY53 pKa = 11.3 EE54 pKa = 4.28 LTLRR58 pKa = 11.84 LTDD61 pKa = 3.51 DD62 pKa = 4.04 TEE64 pKa = 4.19 IQALNAQYY72 pKa = 10.53 RR73 pKa = 11.84 QVDD76 pKa = 3.81 RR77 pKa = 11.84 PTDD80 pKa = 3.47 VLAFAVLEE88 pKa = 4.42 GEE90 pKa = 4.41 TAQILSQIDD99 pKa = 3.81 SLPVYY104 pKa = 10.27 LGDD107 pKa = 3.26 IVISIEE113 pKa = 4.33 TASHH117 pKa = 4.85 QAASKK122 pKa = 10.18 GHH124 pKa = 5.9 SLSQEE129 pKa = 4.35 VTWLAAHH136 pKa = 7.03 GLLHH140 pKa = 6.81 LLGWDD145 pKa = 3.96 HH146 pKa = 7.4 PDD148 pKa = 3.41 EE149 pKa = 5.17 ASLLSMLDD157 pKa = 3.27 QQEE160 pKa = 4.26 ALLAAVGFAGTTAA173 pKa = 3.46
Molecular weight: 18.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.605
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A1Q8ZHN0|A0A1Q8ZHN0_9CYAN Squalene--hopene cyclase OS=Leptolyngbya sp. 'hensonii' OX=1922337 GN=BST81_03925 PE=3 SV=1
MM1 pKa = 7.54 TKK3 pKa = 9.07 RR4 pKa = 11.84 TLHH7 pKa = 4.58 GTSRR11 pKa = 11.84 KK12 pKa = 8.94 RR13 pKa = 11.84 KK14 pKa = 7.75 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TKK26 pKa = 10.27 NGQRR30 pKa = 11.84 VLKK33 pKa = 10.43 ARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.26 GRR40 pKa = 11.84 LRR42 pKa = 11.84 LVVAA46 pKa = 5.09
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5119
0
5119
1680074
24
2961
328.2
36.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.043 ± 0.04
1.007 ± 0.011
4.948 ± 0.023
5.89 ± 0.032
3.654 ± 0.022
7.051 ± 0.034
2.036 ± 0.017
5.828 ± 0.03
3.427 ± 0.026
11.753 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.953 ± 0.014
3.249 ± 0.024
5.426 ± 0.031
5.994 ± 0.031
6.03 ± 0.029
5.988 ± 0.029
5.692 ± 0.031
6.718 ± 0.026
1.476 ± 0.015
2.833 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here