Soboliphyme baturini
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A183J7P8|A0A183J7P8_9BILA Uncharacterized protein OS=Soboliphyme baturini OX=241478 GN=SBAD_LOCUS11896 PE=4 SV=1
MM1 pKa = 7.29 VVDD4 pKa = 4.27 GAIGGGDD11 pKa = 3.4 SMHH14 pKa = 6.48 SVWSSTTKK22 pKa = 10.99 AEE24 pKa = 5.48 DD25 pKa = 3.62 IVPDD29 pKa = 4.32 DD30 pKa = 5.6 DD31 pKa = 5.8 GFDD34 pKa = 4.52 DD35 pKa = 3.69 VWSCGGGGLYY45 pKa = 9.97 PXXXXXXXXXXXXXAIGSCTPFSFGGSLMTDD76 pKa = 3.03 THH78 pKa = 6.55 DD79 pKa = 3.76 LRR81 pKa = 11.84 SPASCWEE88 pKa = 3.97 DD89 pKa = 3.21 VSYY92 pKa = 11.11 GRR94 pKa = 11.84 SGGGYY99 pKa = 9.17 IGSDD103 pKa = 3.21 DD104 pKa = 4.61 RR105 pKa = 11.84 RR106 pKa = 11.84 WKK108 pKa = 9.3 TPAITRR114 pKa = 11.84 QCHH117 pKa = 6.44 DD118 pKa = 4.02 STSSTLDD125 pKa = 3.2 DD126 pKa = 2.96 SAYY129 pKa = 10.57 RR130 pKa = 11.84 VDD132 pKa = 5.02 PYY134 pKa = 11.28 LLSLIDD140 pKa = 5.72 DD141 pKa = 4.33 EE142 pKa = 5.08 TDD144 pKa = 3.61 PDD146 pKa = 4.59 PDD148 pKa = 4.26 PLVVTCSSSDD158 pKa = 3.53 SGSGGAASCSKK169 pKa = 10.46 SSEE172 pKa = 4.31 RR173 pKa = 11.84 PEE175 pKa = 4.09 STPSDD180 pKa = 3.58 TTDD183 pKa = 2.82 VYY185 pKa = 11.77 SPFSGG190 pKa = 3.42
Molecular weight: 18.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.592
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.834
Rodwell 3.656
Grimsley 3.503
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.961
Patrickios 1.138
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A183IKG3|A0A183IKG3_9BILA Uncharacterized protein OS=Soboliphyme baturini OX=241478 GN=SBAD_LOCUS4109 PE=4 SV=1
MM1 pKa = 7.43 RR2 pKa = 11.84 QGSRR6 pKa = 11.84 SGLSLLRR13 pKa = 11.84 HH14 pKa = 5.69 ILSLRR19 pKa = 11.84 LWSSPNGPARR29 pKa = 11.84 AQTTWRR35 pKa = 11.84 IRR37 pKa = 3.28
Molecular weight: 4.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13153
0
13153
3707980
29
6617
281.9
31.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.681 ± 0.02
2.714 ± 0.021
5.293 ± 0.018
5.914 ± 0.027
4.535 ± 0.018
5.392 ± 0.025
2.399 ± 0.009
5.096 ± 0.02
5.451 ± 0.024
9.451 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.01
4.201 ± 0.016
4.687 ± 0.023
3.84 ± 0.017
6.057 ± 0.02
8.508 ± 0.026
5.371 ± 0.018
7.015 ± 0.02
1.18 ± 0.008
3.11 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here