Tautonia sociabilis
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432MLD5|A0A432MLD5_9BACT DNA-directed RNA polymerase subunit omega OS=Tautonia sociabilis OX=2080755 GN=rpoZ PE=3 SV=1
MM1 pKa = 7.38 QNVQDD6 pKa = 3.89 PMGPIIATRR15 pKa = 11.84 PMVWLRR21 pKa = 11.84 ADD23 pKa = 3.48 GAEE26 pKa = 3.57 IPAFVEE32 pKa = 3.43 IGMPYY37 pKa = 9.8 QLPPDD42 pKa = 3.96 VPGEE46 pKa = 4.21 PGDD49 pKa = 3.45 WACRR53 pKa = 11.84 VRR55 pKa = 11.84 TRR57 pKa = 11.84 GLGDD61 pKa = 3.22 NNFYY65 pKa = 10.15 TVYY68 pKa = 10.21 GVDD71 pKa = 4.99 GIQVLYY77 pKa = 10.38 LALSMAGTLVSNSIVAGDD95 pKa = 4.38 LDD97 pKa = 3.88 WSEE100 pKa = 4.16 VPNFGFPPPPEE111 pKa = 4.12 QPDD114 pKa = 3.45 GDD116 pKa = 4.44 EE117 pKa = 4.59 GCEE120 pKa = 4.19 GCPPSPP126 pKa = 4.15
Molecular weight: 13.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.757
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A432MM77|A0A432MM77_9BACT Uncharacterized protein OS=Tautonia sociabilis OX=2080755 GN=TsocGM_06370 PE=4 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 LVAALRR8 pKa = 11.84 LIARR12 pKa = 11.84 LLIAALRR19 pKa = 11.84 LIAALLIAALLVAALLVPALRR40 pKa = 11.84 LIAALLIAARR50 pKa = 11.84 LVPALRR56 pKa = 11.84 LIARR60 pKa = 11.84 LLIAALLVAAPRR72 pKa = 11.84 LVPTSRR78 pKa = 11.84 LIAGRR83 pKa = 11.84 LVAALRR89 pKa = 11.84 LVAGLLVFALGLIAGRR105 pKa = 11.84 RR106 pKa = 11.84 VARR109 pKa = 11.84 LRR111 pKa = 11.84 RR112 pKa = 11.84 LVLLIGGRR120 pKa = 11.84 GVLRR124 pKa = 11.84 RR125 pKa = 11.84 LTILVEE131 pKa = 4.1 LVEE134 pKa = 4.78 GLAKK138 pKa = 10.09 PVEE141 pKa = 4.3 PFLVLGG147 pKa = 4.26
Molecular weight: 15.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.135
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.857
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4914
0
4914
1862055
30
3548
378.9
41.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.613 ± 0.047
0.895 ± 0.013
6.227 ± 0.028
6.348 ± 0.037
3.295 ± 0.02
9.169 ± 0.037
1.926 ± 0.017
4.297 ± 0.023
2.033 ± 0.025
10.592 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.771 ± 0.015
2.054 ± 0.025
7.059 ± 0.042
2.621 ± 0.019
8.956 ± 0.037
5.755 ± 0.027
4.575 ± 0.023
7.312 ± 0.03
1.458 ± 0.016
2.043 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here