Streptococcus satellite phage Javan757
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZWW4|A0A4D5ZWW4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan757 OX=2558852 GN=JavanS757_0010 PE=4 SV=1
MM1 pKa = 7.24 KK2 pKa = 10.52 VKK4 pKa = 10.49 LFEE7 pKa = 3.94 YY8 pKa = 8.31 TRR10 pKa = 11.84 RR11 pKa = 11.84 WEE13 pKa = 4.07 KK14 pKa = 11.04 EE15 pKa = 3.96 DD16 pKa = 3.66 FEE18 pKa = 5.74 KK19 pKa = 11.1 EE20 pKa = 3.45 IDD22 pKa = 3.75 DD23 pKa = 4.32 FMATVEE29 pKa = 4.06 VLDD32 pKa = 4.19 VKK34 pKa = 10.96 FSGTPSSEE42 pKa = 4.05 SFNSVAIVLVLYY54 pKa = 10.53 RR55 pKa = 4.39
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.907
IPC2_protein 4.914
IPC_protein 4.685
Toseland 4.533
ProMoST 4.774
Dawson 4.609
Bjellqvist 4.762
Wikipedia 4.469
Rodwell 4.52
Grimsley 4.444
Solomon 4.596
Lehninger 4.558
Nozaki 4.724
DTASelect 4.825
Thurlkill 4.533
EMBOSS 4.482
Sillero 4.787
Patrickios 4.342
IPC_peptide 4.609
IPC2_peptide 4.774
IPC2.peptide.svr19 4.796
Protein with the highest isoelectric point:
>tr|A0A4D6A1A0|A0A4D6A1A0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan757 OX=2558852 GN=JavanS757_0019 PE=4 SV=1
MM1 pKa = 7.51 SSLSACHH8 pKa = 5.87 SARR11 pKa = 11.84 SRR13 pKa = 11.84 SEE15 pKa = 3.21 IRR17 pKa = 11.84 GLKK20 pKa = 9.24 TSLICLSFLRR30 pKa = 11.84 GLRR33 pKa = 11.84 RR34 pKa = 11.84 VFIALIYY41 pKa = 9.81 LINDD45 pKa = 3.64 RR46 pKa = 11.84 GDD48 pKa = 3.02 IATFSAFARR57 pKa = 11.84 SSTHH61 pKa = 4.18 ITLPFVRR68 pKa = 11.84 HH69 pKa = 6.47 IYY71 pKa = 9.88 CHH73 pKa = 5.77 YY74 pKa = 9.96 LSKK77 pKa = 10.62 ISPFLTLGKK86 pKa = 9.35 PLYY89 pKa = 10.03 LCKK92 pKa = 10.04 TLVFLSMM99 pKa = 4.68
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.692
IPC_protein 10.35
Toseland 10.262
ProMoST 10.058
Dawson 10.482
Bjellqvist 10.233
Wikipedia 10.687
Rodwell 10.701
Grimsley 10.555
Solomon 10.54
Lehninger 10.496
Nozaki 10.35
DTASelect 10.204
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.409
Patrickios 10.423
IPC_peptide 10.54
IPC2_peptide 9.545
IPC2.peptide.svr19 8.337
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3490
48
516
174.5
20.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.072 ± 0.45
0.745 ± 0.151
5.358 ± 0.356
8.453 ± 0.633
4.842 ± 0.374
4.699 ± 0.323
2.178 ± 0.261
7.049 ± 0.606
9.828 ± 0.605
10.057 ± 0.523
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.321 ± 0.301
5.415 ± 0.68
3.352 ± 0.547
3.467 ± 0.336
5.387 ± 0.425
5.788 ± 0.366
5.645 ± 0.394
5.072 ± 0.355
0.802 ± 0.19
4.47 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here