Spongiivirga citrea
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M0CLF3|A0A6M0CLF3_9FLAO Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Spongiivirga citrea OX=1481457 GN=GWK10_05620 PE=3 SV=1
PP1 pKa = 8.03 DD2 pKa = 5.06 PFDD5 pKa = 3.84 TNVPNTYY12 pKa = 8.88 TLTYY16 pKa = 9.93 SATGATNVTRR26 pKa = 11.84 TVVVNATPVASFTVDD41 pKa = 2.73 QSTITEE47 pKa = 3.96 GSMVNFDD54 pKa = 4.4 ASASTASSAITYY66 pKa = 9.7 NWDD69 pKa = 3.38 FGDD72 pKa = 4.65 GSPIQQTTNPTISHH86 pKa = 6.02 QYY88 pKa = 8.83 SSNVGSPFTATLSIEE103 pKa = 4.29 DD104 pKa = 4.19 ANGCTSATNASQEE117 pKa = 4.07 ITVNPATSPFIDD129 pKa = 4.74 LGALPATVTLCQGAIFTDD147 pKa = 4.02 TVSAEE152 pKa = 4.21 DD153 pKa = 3.82 GNGNIIPVTTTSSPDD168 pKa = 3.41 PFDD171 pKa = 3.8 TNLPGTYY178 pKa = 9.38 TLTYY182 pKa = 9.42 SAAGATDD189 pKa = 3.29 MTRR192 pKa = 11.84 TVVVNTTPVASFNVDD207 pKa = 2.75 QLTITEE213 pKa = 4.34 GEE215 pKa = 4.43 TVNFDD220 pKa = 3.54 ASASTASSAITYY232 pKa = 7.45 TWNFGDD238 pKa = 4.58 GSPIQTTTNPTVDD251 pKa = 3.25 HH252 pKa = 6.81 TYY254 pKa = 9.03 STSAGSPFTTTLTIEE269 pKa = 4.64 DD270 pKa = 4.55 ADD272 pKa = 3.88 GCTNTNTASQEE283 pKa = 3.82 IIVNVTGSATVLINSAKK300 pKa = 8.47 NASYY304 pKa = 9.09 VTEE307 pKa = 4.18 NVQITIDD314 pKa = 3.31 IDD316 pKa = 3.56 EE317 pKa = 4.67 TNGGNGPYY325 pKa = 9.04 TAGYY329 pKa = 10.08 RR330 pKa = 11.84 NATGSFDD337 pKa = 3.87 LSNGTSINVDD347 pKa = 2.95 TDD349 pKa = 3.07 ISIGNNITNWTFIGNAAFDD368 pKa = 4.06 ASFEE372 pKa = 4.29 IYY374 pKa = 10.08 VKK376 pKa = 10.84 NGAGEE381 pKa = 4.55 EE382 pKa = 4.23 IGSSTINIAVQTFTINLIPDD402 pKa = 3.99 TDD404 pKa = 4.82 PINAEE409 pKa = 3.96 VGNSVDD415 pKa = 3.82 FTLFIGEE422 pKa = 4.23 VNGQIGNQYY431 pKa = 10.66 NITINTDD438 pKa = 2.5 QGTDD442 pKa = 3.27 GTINAGGDD450 pKa = 3.54 SSTGTLTINNQSPGSPISISYY471 pKa = 10.3 IGTTATNHH479 pKa = 6.55 AISVSVVPVSFNGLSRR495 pKa = 11.84 NEE497 pKa = 3.93 NRR499 pKa = 11.84 NINYY503 pKa = 7.69 TSTAFPFTFSASTVTVNPFLFFNNEE528 pKa = 2.75 IDD530 pKa = 3.33 INLNITPAGPVGGGQHH546 pKa = 5.4 EE547 pKa = 4.5 VRR549 pKa = 11.84 FTGDD553 pKa = 3.08 LAGGQILDD561 pKa = 3.63 QSNNTVSLNTWTSVNNSNLTVWKK584 pKa = 9.64 FRR586 pKa = 11.84 AGTSIGIADD595 pKa = 4.48 LNFEE599 pKa = 4.59 ARR601 pKa = 11.84 NASGGTVTGNPQNINIKK618 pKa = 10.27 IIDD621 pKa = 3.84 FSITVNPTTTITEE634 pKa = 4.21 TAGTQVTNLSVRR646 pKa = 11.84 IDD648 pKa = 3.54 PTDD651 pKa = 3.06 TSFISAYY658 pKa = 10.37 DD659 pKa = 3.37 LTIASDD665 pKa = 3.49 QADD668 pKa = 4.34 GIFNSGDD675 pKa = 3.22 THH677 pKa = 6.17 ITTTVNGGASFNFNYY692 pKa = 10.23 TGTSVNLHH700 pKa = 6.04 NLSISATPVGQTEE713 pKa = 4.98 PIRR716 pKa = 11.84 TSAFRR721 pKa = 11.84 IDD723 pKa = 4.01 YY724 pKa = 10.23 VAGNQPPIAQNDD736 pKa = 3.83 SFIIQQNSSQNLLLVYY752 pKa = 10.32 ADD754 pKa = 3.8 NGSGIDD760 pKa = 4.12 EE761 pKa = 4.96 DD762 pKa = 4.71 PEE764 pKa = 5.4 NDD766 pKa = 4.22 DD767 pKa = 5.77 FIINQIPSNPSKK779 pKa = 11.02 GITAIQGDD787 pKa = 4.84 AINYY791 pKa = 8.33 VPTNGEE797 pKa = 3.91 FGNDD801 pKa = 2.7 TFTYY805 pKa = 10.26 NLEE808 pKa = 4.74 DD809 pKa = 3.31 INGNVSNDD817 pKa = 2.87 ATVSVFINRR826 pKa = 11.84 PPTINPQSVTRR837 pKa = 11.84 LISDD841 pKa = 3.8 RR842 pKa = 11.84 SGTLLISINSLTNDD856 pKa = 3.73 PDD858 pKa = 3.35 GHH860 pKa = 5.67 TVLLNAVGTPSNNGGTAIIGTGQQTIVFSGNGGTWATSTFTYY902 pKa = 10.07 QVSDD906 pKa = 3.83 GFGGTATGTVIVEE919 pKa = 4.37 FDD921 pKa = 4.06 PGTGPDD927 pKa = 3.93 PCDD930 pKa = 3.27 NCPPGTSCTNCDD942 pKa = 2.86 ICISPMQACPQAPPKK957 pKa = 10.29 EE958 pKa = 4.01 GRR960 pKa = 11.84 NPIKK964 pKa = 10.55 VEE966 pKa = 3.87 KK967 pKa = 9.9 VNKK970 pKa = 9.84 GKK972 pKa = 10.74 AKK974 pKa = 10.11 IEE976 pKa = 4.11 KK977 pKa = 9.55 QQ978 pKa = 3.17
Molecular weight: 102.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 1.062
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A6M0CSK8|A0A6M0CSK8_9FLAO Type IX secretion system membrane protein PorP/SprF (Fragment) OS=Spongiivirga citrea OX=1481457 GN=GWK10_16855 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.17 RR21 pKa = 11.84 MASVSGRR28 pKa = 11.84 KK29 pKa = 9.06 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.52 KK41 pKa = 10.3 ISVSSDD47 pKa = 2.85 PRR49 pKa = 11.84 HH50 pKa = 6.1 KK51 pKa = 10.6 KK52 pKa = 9.83
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3588
0
3588
1259079
34
7025
350.9
39.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.575 ± 0.041
0.727 ± 0.015
5.995 ± 0.057
6.357 ± 0.042
5.198 ± 0.035
6.638 ± 0.047
1.693 ± 0.025
7.718 ± 0.036
7.346 ± 0.086
9.12 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.028
6.086 ± 0.042
3.438 ± 0.033
3.464 ± 0.022
3.575 ± 0.036
6.538 ± 0.032
6.038 ± 0.102
6.383 ± 0.043
1.087 ± 0.016
3.887 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here