Atlantibacter hermannii NBRC 105704
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H5V0I1|H5V0I1_ATLHE DNA mismatch repair protein MutS OS=Atlantibacter hermannii NBRC 105704 OX=1115512 GN=mutS PE=3 SV=1
MM1 pKa = 7.62 NMGLFYY7 pKa = 10.89 GSSTCYY13 pKa = 10.49 TEE15 pKa = 3.84 MAAEE19 pKa = 5.12 KK20 pKa = 10.0 IRR22 pKa = 11.84 DD23 pKa = 3.65 IIGPEE28 pKa = 4.02 LVTLHH33 pKa = 6.14 NLKK36 pKa = 10.64 DD37 pKa = 3.68 DD38 pKa = 4.36 APALMEE44 pKa = 4.2 QYY46 pKa = 10.98 DD47 pKa = 4.12 VLILGIPTWDD57 pKa = 3.26 FGEE60 pKa = 4.22 IQEE63 pKa = 4.35 DD64 pKa = 3.97 WEE66 pKa = 4.89 AIWDD70 pKa = 3.89 QLDD73 pKa = 3.66 TLDD76 pKa = 4.17 LAGKK80 pKa = 9.72 IVALYY85 pKa = 11.07 GMGDD89 pKa = 3.29 QLGYY93 pKa = 11.02 GEE95 pKa = 4.87 WFLDD99 pKa = 3.74 ALGMLHH105 pKa = 7.44 DD106 pKa = 4.34 KK107 pKa = 11.0 LMPKK111 pKa = 9.82 GVKK114 pKa = 9.57 FVGYY118 pKa = 9.0 WPTEE122 pKa = 4.03 GYY124 pKa = 10.61 EE125 pKa = 4.19 FTSPKK130 pKa = 10.02 PVIADD135 pKa = 3.41 GQLFVGLALDD145 pKa = 3.64 EE146 pKa = 4.38 TNQYY150 pKa = 10.89 DD151 pKa = 4.3 LSDD154 pKa = 3.47 EE155 pKa = 5.91 RR156 pKa = 11.84 IQQWCEE162 pKa = 3.62 QILGEE167 pKa = 4.17 MAEE170 pKa = 4.4 HH171 pKa = 7.09 FSS173 pKa = 3.73
Molecular weight: 19.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.948
IPC_protein 3.91
Toseland 3.719
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.202
Thurlkill 3.757
EMBOSS 3.821
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|H5V3R1|H5V3R1_ATLHE AAA_15 domain-containing protein OS=Atlantibacter hermannii NBRC 105704 OX=1115512 GN=EH105704_07_01870 PE=4 SV=1
MM1 pKa = 7.05 LTVPALCWLCGMPLALSHH19 pKa = 6.84 WGLCSRR25 pKa = 11.84 CVAALPAPPPACPVCGLPAVVTTMPCGRR53 pKa = 11.84 CLHH56 pKa = 6.57 KK57 pKa = 10.73 PPAWEE62 pKa = 3.71 RR63 pKa = 11.84 LLYY66 pKa = 8.74 VTDD69 pKa = 3.59 YY70 pKa = 10.23 CQPLSALVQALKK82 pKa = 9.87 FHH84 pKa = 6.71 RR85 pKa = 11.84 QTALAKK91 pKa = 9.63 PLARR95 pKa = 11.84 LMLLRR100 pKa = 11.84 ARR102 pKa = 11.84 EE103 pKa = 4.11 ALRR106 pKa = 11.84 EE107 pKa = 3.96 QAFPRR112 pKa = 11.84 PDD114 pKa = 3.38 LVIGVPLHH122 pKa = 7.01 RR123 pKa = 11.84 RR124 pKa = 11.84 RR125 pKa = 11.84 AWHH128 pKa = 6.6 RR129 pKa = 11.84 GFNQSALLAKK139 pKa = 10.14 PLARR143 pKa = 11.84 WLGCRR148 pKa = 11.84 YY149 pKa = 10.0 DD150 pKa = 4.13 DD151 pKa = 3.83 RR152 pKa = 11.84 VLRR155 pKa = 11.84 RR156 pKa = 11.84 IRR158 pKa = 11.84 STPVQHH164 pKa = 6.68 HH165 pKa = 6.3 LSARR169 pKa = 11.84 LRR171 pKa = 11.84 KK172 pKa = 9.53 KK173 pKa = 10.13 NLRR176 pKa = 11.84 AAFRR180 pKa = 11.84 LEE182 pKa = 3.94 LPVRR186 pKa = 11.84 DD187 pKa = 3.64 LHH189 pKa = 8.32 IVIVDD194 pKa = 3.75 DD195 pKa = 4.22 VVTTGSTVGEE205 pKa = 4.18 MAKK208 pKa = 10.44 LLHH211 pKa = 6.17 RR212 pKa = 11.84 HH213 pKa = 5.48 GAATVQVWCLCRR225 pKa = 11.84 TLL227 pKa = 4.39
Molecular weight: 25.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.472
IPC_protein 10.321
Toseland 10.628
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.496
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.76
Solomon 10.877
Lehninger 10.833
Nozaki 10.701
DTASelect 10.452
Thurlkill 10.643
EMBOSS 11.052
Sillero 10.672
Patrickios 10.526
IPC_peptide 10.877
IPC2_peptide 10.043
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4159
0
4159
1323175
29
4081
318.1
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.027 ± 0.046
1.083 ± 0.014
5.273 ± 0.031
5.602 ± 0.034
3.834 ± 0.03
7.505 ± 0.044
2.29 ± 0.02
5.703 ± 0.031
4.088 ± 0.032
10.773 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.765 ± 0.02
3.733 ± 0.029
4.542 ± 0.029
4.455 ± 0.033
5.751 ± 0.033
5.791 ± 0.029
5.404 ± 0.033
7.149 ± 0.033
1.526 ± 0.018
2.705 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here