Colwellia chukchiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7RFX6|A0A1H7RFX6_9GAMM Glutamine synthetase OS=Colwellia chukchiensis OX=641665 GN=SAMN05216262_11489 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.25KK3 pKa = 9.53IALAVTFASILSTNVQADD21 pKa = 3.27ALGIYY26 pKa = 10.28LGGQVWDD33 pKa = 4.02NQASGVFGEE42 pKa = 4.89SSSQIDD48 pKa = 3.81FNLADD53 pKa = 3.81KK54 pKa = 10.28KK55 pKa = 11.01QNSFFLAFEE64 pKa = 4.61HH65 pKa = 6.76PLPIIPNIKK74 pKa = 9.47IASTSLEE81 pKa = 4.42TEE83 pKa = 4.29GEE85 pKa = 4.25TTLANDD91 pKa = 4.04FEE93 pKa = 5.06FEE95 pKa = 4.26GEE97 pKa = 4.27TFRR100 pKa = 11.84EE101 pKa = 4.31GSMVNADD108 pKa = 3.66FNISYY113 pKa = 10.91VDD115 pKa = 3.26YY116 pKa = 9.62TLYY119 pKa = 11.34YY120 pKa = 10.64EE121 pKa = 5.86LFDD124 pKa = 4.64NDD126 pKa = 4.45LLSFDD131 pKa = 4.49FGLTGRR137 pKa = 11.84DD138 pKa = 3.21FDD140 pKa = 5.14GDD142 pKa = 3.5ITVAATVNLDD152 pKa = 3.66SGPVTQSGTLEE163 pKa = 3.96VTEE166 pKa = 4.64IVPMLYY172 pKa = 10.21VATNVGLPLTGLNLYY187 pKa = 10.05AQGNFLSFDD196 pKa = 3.1SHH198 pKa = 5.51TLYY201 pKa = 10.68DD202 pKa = 3.82YY203 pKa = 11.14EE204 pKa = 4.53VGVSYY209 pKa = 10.76EE210 pKa = 4.57LIDD213 pKa = 3.91NLAVDD218 pKa = 3.96VNINAGYY225 pKa = 10.02RR226 pKa = 11.84AVKK229 pKa = 10.65LEE231 pKa = 4.52LEE233 pKa = 4.67DD234 pKa = 6.24LSDD237 pKa = 4.16LYY239 pKa = 11.4TNIEE243 pKa = 3.86FDD245 pKa = 4.09GVFLGTTIHH254 pKa = 6.76FF255 pKa = 4.43

Molecular weight: 28.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7PST0|A0A1H7PST0_9GAMM Phosphoenolpyruvate carboxykinase (ATP) OS=Colwellia chukchiensis OX=641665 GN=pckA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34AGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.46GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3563

0

3563

1168915

26

1873

328.1

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.462 ± 0.043

1.011 ± 0.015

5.321 ± 0.035

5.58 ± 0.039

4.197 ± 0.026

6.252 ± 0.041

2.283 ± 0.021

6.749 ± 0.032

5.62 ± 0.036

10.501 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.022

4.729 ± 0.03

3.667 ± 0.025

5.228 ± 0.044

4.195 ± 0.028

6.676 ± 0.033

5.315 ± 0.027

6.56 ± 0.034

1.144 ± 0.014

3.118 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski