Beihai tombus-like virus 18
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFP7|A0A1L3KFP7_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 18 OX=1922721 PE=4 SV=1
MM1 pKa = 7.35 PTNKK5 pKa = 10.11 GNTKK9 pKa = 9.79 KK10 pKa = 10.99 ANGNANMKK18 pKa = 10.79 KK19 pKa = 9.65 MMAKK23 pKa = 9.95 SSSKK27 pKa = 10.19 SGPRR31 pKa = 11.84 SGITPKK37 pKa = 10.66 VNLGKK42 pKa = 10.33 QKK44 pKa = 11.09 SSVKK48 pKa = 9.39 WGNDD52 pKa = 2.71 RR53 pKa = 11.84 CSVSGEE59 pKa = 4.21 DD60 pKa = 5.51 FIHH63 pKa = 6.12 TLQLNEE69 pKa = 4.79 LPNSRR74 pKa = 11.84 NFAGAVLFPQYY85 pKa = 10.79 VNPWRR90 pKa = 11.84 LPGTRR95 pKa = 11.84 LTQFANLFQRR105 pKa = 11.84 FKK107 pKa = 10.27 FTRR110 pKa = 11.84 FTVKK114 pKa = 10.55 YY115 pKa = 8.88 IPAVPATVAGQIIMCTDD132 pKa = 3.66 TDD134 pKa = 4.04 ATWAPIGDD142 pKa = 4.05 TQDD145 pKa = 3.5 VIRR148 pKa = 11.84 MMMAHH153 pKa = 7.9 KK154 pKa = 10.23 DD155 pKa = 3.07 RR156 pKa = 11.84 IMFHH160 pKa = 6.76 VFDD163 pKa = 4.2 NAKK166 pKa = 10.35 AVIPGTSKK174 pKa = 10.9 PEE176 pKa = 3.99 YY177 pKa = 9.06 MCDD180 pKa = 3.31 AQGQDD185 pKa = 3.2 VRR187 pKa = 11.84 VNNQGVFWAALVSPVVKK204 pKa = 10.34 QDD206 pKa = 3.03 GAEE209 pKa = 3.76 WTGAVGSFVLEE220 pKa = 3.94 WEE222 pKa = 4.79 VIFTAARR229 pKa = 11.84 ILTPQRR235 pKa = 11.84 ITSQSTAKK243 pKa = 10.52 AQWTGDD249 pKa = 3.64 DD250 pKa = 4.72 DD251 pKa = 4.76 YY252 pKa = 11.69 GASLAQVTGAEE263 pKa = 4.81 PNTQTCVAIFRR274 pKa = 11.84 DD275 pKa = 3.7 VGQAIGMTSGTPYY288 pKa = 10.8 FLGSRR293 pKa = 11.84 PLSTRR298 pKa = 11.84 VVPSEE303 pKa = 3.7 YY304 pKa = 9.71 EE305 pKa = 3.42 RR306 pKa = 11.84 AYY308 pKa = 10.97 NVYY311 pKa = 8.58 GTLRR315 pKa = 11.84 GAQQLDD321 pKa = 3.87 KK322 pKa = 11.29 LDD324 pKa = 5.36 LIPVEE329 pKa = 5.06 GILTAVDD336 pKa = 4.75 FIWWPIGPEE345 pKa = 3.53 HH346 pKa = 6.45 GTRR349 pKa = 11.84 NSTIDD354 pKa = 3.78 AKK356 pKa = 11.15 VQMSGLSNTTDD367 pKa = 3.03 TGLLNVDD374 pKa = 4.45 YY375 pKa = 11.21 NNTDD379 pKa = 3.13 PRR381 pKa = 11.84 RR382 pKa = 11.84 QADD385 pKa = 4.12 EE386 pKa = 4.36 YY387 pKa = 10.83 FVRR390 pKa = 11.84 ATGALPEE397 pKa = 5.35 DD398 pKa = 4.28 NADD401 pKa = 5.09 DD402 pKa = 4.54 PSDD405 pKa = 4.02 LNGVALIYY413 pKa = 10.43 TDD415 pKa = 4.0 EE416 pKa = 4.68 NDD418 pKa = 3.08 VTRR421 pKa = 11.84 TVEE424 pKa = 3.89 QNQMLIYY431 pKa = 10.6 RR432 pKa = 11.84 KK433 pKa = 5.68 TTPRR437 pKa = 11.84 ALATAMGWTDD447 pKa = 3.24 VQFQNIYY454 pKa = 10.23 GFSVNEE460 pKa = 3.85 KK461 pKa = 10.08 AFVGVLITALGHH473 pKa = 5.37 FVRR476 pKa = 11.84 AVSILAKK483 pKa = 9.75 AARR486 pKa = 11.84 IALAIIRR493 pKa = 11.84 EE494 pKa = 4.25 VKK496 pKa = 10.0 EE497 pKa = 3.91 YY498 pKa = 10.5 EE499 pKa = 3.94 SEE501 pKa = 3.96 FFKK504 pKa = 10.92 RR505 pKa = 11.84 INFSAASEE513 pKa = 4.2 LEE515 pKa = 4.21 RR516 pKa = 11.84 ANGLHH521 pKa = 6.42 SGHH524 pKa = 6.34 TLSLVKK530 pKa = 10.62 NPPRR534 pKa = 11.84 QIPSGGFRR542 pKa = 11.84 RR543 pKa = 11.84 EE544 pKa = 3.73 KK545 pKa = 10.6 ASNVXILGTGDD556 pKa = 3.54 STSLQLVEE564 pKa = 4.72 PCPEE568 pKa = 4.2 PGVFVFRR575 pKa = 11.84 EE576 pKa = 4.25 VNDD579 pKa = 3.46 EE580 pKa = 4.04 HH581 pKa = 7.71 QGNLSEE587 pKa = 5.22 PDD589 pKa = 3.83 DD590 pKa = 4.71 SDD592 pKa = 4.24 SEE594 pKa = 4.96 SIASSDD600 pKa = 3.44 PLNEE604 pKa = 3.77 HH605 pKa = 6.14 FVFRR609 pKa = 11.84 RR610 pKa = 11.84 NDD612 pKa = 3.41 DD613 pKa = 3.11 VCYY616 pKa = 10.44 VVVNIVNNYY625 pKa = 9.04 IFEE628 pKa = 5.3 CDD630 pKa = 3.1 THH632 pKa = 8.54 FDD634 pKa = 4.08 FPPGSVWVPDD644 pKa = 3.37 LTVFGIEE651 pKa = 4.16 PNPGPVCHH659 pKa = 6.62 NCGKK663 pKa = 9.81 NGHH666 pKa = 6.32 IARR669 pKa = 11.84 EE670 pKa = 4.1 CHH672 pKa = 5.5 APKK675 pKa = 10.5 AKK677 pKa = 10.26 RR678 pKa = 11.84 EE679 pKa = 3.96 TRR681 pKa = 11.84 KK682 pKa = 7.73 TVNKK686 pKa = 10.19 NLGPRR691 pKa = 11.84 RR692 pKa = 11.84 DD693 pKa = 3.97 GQNKK697 pKa = 8.64 LAASVRR703 pKa = 11.84 EE704 pKa = 3.96 GLAAVAGASDD714 pKa = 3.54 AAKK717 pKa = 10.43 EE718 pKa = 3.92 KK719 pKa = 10.69 DD720 pKa = 3.26 AEE722 pKa = 4.81 AIDD725 pKa = 3.77 RR726 pKa = 11.84 LKK728 pKa = 10.97 EE729 pKa = 3.96 VIKK732 pKa = 10.65 EE733 pKa = 4.13 VVKK736 pKa = 8.59 TQKK739 pKa = 10.73 DD740 pKa = 3.7 EE741 pKa = 4.15 EE742 pKa = 4.29 EE743 pKa = 4.9 KK744 pKa = 10.79 DD745 pKa = 3.6 VKK747 pKa = 10.29 TYY749 pKa = 10.58 TPLPGNFTSVIEE761 pKa = 4.38 HH762 pKa = 7.14 KK763 pKa = 11.15 YY764 pKa = 10.11 FDD766 pKa = 4.5 HH767 pKa = 7.63 PDD769 pKa = 4.73 FIPTYY774 pKa = 9.87 KK775 pKa = 9.08 VTSPVRR781 pKa = 11.84 ILYY784 pKa = 7.63 HH785 pKa = 5.2 TLILTLKK792 pKa = 8.74 LCKK795 pKa = 10.06 YY796 pKa = 9.87 YY797 pKa = 10.06 PLFGSAVVYY806 pKa = 10.6 LLLSLLMKK814 pKa = 10.29 RR815 pKa = 11.84 INSIMPTGPAFMKK828 pKa = 10.09 PISSLFPLMAKK839 pKa = 10.26 CFVTLPATFWSLGFSYY855 pKa = 10.53 GFAFTYY861 pKa = 9.68 VLRR864 pKa = 11.84 HH865 pKa = 5.33 LKK867 pKa = 10.21 FRR869 pKa = 11.84 TTYY872 pKa = 8.05 EE873 pKa = 4.43 TYY875 pKa = 10.73 CKK877 pKa = 10.46 VLYY880 pKa = 10.46 QEE882 pKa = 5.59 AEE884 pKa = 3.99 GEE886 pKa = 4.12 MRR888 pKa = 11.84 PEE890 pKa = 4.02 RR891 pKa = 11.84 MHH893 pKa = 6.34 HH894 pKa = 6.1 QDD896 pKa = 3.83 LIKK899 pKa = 11.06 YY900 pKa = 9.47 SDD902 pKa = 3.58 PKK904 pKa = 8.45 TVKK907 pKa = 10.67 VKK909 pKa = 9.66 VTDD912 pKa = 4.05 KK913 pKa = 11.11 LICQDD918 pKa = 4.58 DD919 pKa = 4.05 LFKK922 pKa = 10.35 IDD924 pKa = 4.0 EE925 pKa = 4.38 EE926 pKa = 4.49 VVEE929 pKa = 4.28 YY930 pKa = 10.44 EE931 pKa = 5.04 RR932 pKa = 11.84 KK933 pKa = 9.26 RR934 pKa = 11.84 QLNVSLEE941 pKa = 4.15 LFSQFSGYY949 pKa = 8.12 TMITAPTKK957 pKa = 9.33 EE958 pKa = 4.49 FYY960 pKa = 10.01 MGLQRR965 pKa = 11.84 DD966 pKa = 3.81 IKK968 pKa = 8.6 RR969 pKa = 11.84 TCSIGFDD976 pKa = 3.34 RR977 pKa = 11.84 MDD979 pKa = 3.22 MARR982 pKa = 11.84 RR983 pKa = 11.84 NIFNDD988 pKa = 2.94 TATYY992 pKa = 7.46 ITAWKK997 pKa = 9.43 LSEE1000 pKa = 3.75 MTKK1003 pKa = 10.04 RR1004 pKa = 11.84 DD1005 pKa = 3.42 SHH1007 pKa = 5.43 EE1008 pKa = 3.9 QPLNN1012 pKa = 3.45
Molecular weight: 113.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.619
IPC2_protein 6.825
IPC_protein 6.795
Toseland 6.766
ProMoST 7.293
Dawson 7.38
Bjellqvist 7.541
Wikipedia 7.322
Rodwell 7.395
Grimsley 6.868
Solomon 7.41
Lehninger 7.424
Nozaki 7.805
DTASelect 7.629
Thurlkill 7.673
EMBOSS 7.688
Sillero 7.849
Patrickios 4.368
IPC_peptide 7.41
IPC2_peptide 7.073
IPC2.peptide.svr19 6.99
Protein with the highest isoelectric point:
>tr|A0A1L3KFP7|A0A1L3KFP7_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 18 OX=1922721 PE=4 SV=1
MM1 pKa = 7.64 SVINKK6 pKa = 8.82 NRR8 pKa = 11.84 VANITRR14 pKa = 11.84 PMVDD18 pKa = 3.27 RR19 pKa = 11.84 DD20 pKa = 4.64 DD21 pKa = 4.2 IVTSATGAMHH31 pKa = 7.22 RR32 pKa = 11.84 VTPVMPDD39 pKa = 2.85 VKK41 pKa = 10.72 EE42 pKa = 3.95 PEE44 pKa = 4.36 EE45 pKa = 3.91 YY46 pKa = 10.33 RR47 pKa = 11.84 KK48 pKa = 9.2 FARR51 pKa = 11.84 KK52 pKa = 8.28 MFEE55 pKa = 3.64 IFLTPLEE62 pKa = 4.0 WDD64 pKa = 3.9 EE65 pKa = 4.29 IIKK68 pKa = 10.6 FEE70 pKa = 4.91 VFIDD74 pKa = 3.83 MCDD77 pKa = 3.79 KK78 pKa = 9.91 PQSWKK83 pKa = 9.57 DD84 pKa = 3.48 TMRR87 pKa = 11.84 EE88 pKa = 3.67 CHH90 pKa = 5.73 YY91 pKa = 11.31 NRR93 pKa = 11.84 VKK95 pKa = 11.11 DD96 pKa = 3.8 FDD98 pKa = 3.88 YY99 pKa = 11.67 EE100 pKa = 3.96 EE101 pKa = 4.79 FYY103 pKa = 10.37 RR104 pKa = 11.84 TNSSHH109 pKa = 7.05 IKK111 pKa = 10.44 EE112 pKa = 4.16 EE113 pKa = 5.98 DD114 pKa = 3.23 YY115 pKa = 10.16 DD116 pKa = 3.62 TWKK119 pKa = 10.34 NARR122 pKa = 11.84 TINGTHH128 pKa = 6.56 RR129 pKa = 11.84 KK130 pKa = 9.96 GYY132 pKa = 10.89 AKK134 pKa = 10.68 DD135 pKa = 3.38 HH136 pKa = 6.42 LSWIARR142 pKa = 11.84 YY143 pKa = 9.54 VKK145 pKa = 10.36 SCEE148 pKa = 3.51 KK149 pKa = 10.4 QIYY152 pKa = 9.0 HH153 pKa = 7.14 KK154 pKa = 11.07 LPGLVKK160 pKa = 10.63 GMTPKK165 pKa = 10.64 EE166 pKa = 3.95 RR167 pKa = 11.84 MEE169 pKa = 4.1 AMYY172 pKa = 10.5 EE173 pKa = 4.17 LGDD176 pKa = 4.33 HH177 pKa = 5.7 MPKK180 pKa = 10.28 TIGDD184 pKa = 3.82 YY185 pKa = 10.4 TSYY188 pKa = 10.53 EE189 pKa = 3.74 ASFKK193 pKa = 9.84 RR194 pKa = 11.84 KK195 pKa = 9.85 KK196 pKa = 9.29 MEE198 pKa = 4.02 CVQFEE203 pKa = 4.35 FYY205 pKa = 11.3 EE206 pKa = 4.72 FMFQKK211 pKa = 10.84 LPFKK215 pKa = 10.34 NKK217 pKa = 9.44 LMEE220 pKa = 4.31 GMRR223 pKa = 11.84 HH224 pKa = 5.73 VIGGKK229 pKa = 9.7 NKK231 pKa = 8.54 MRR233 pKa = 11.84 FKK235 pKa = 11.06 NFTWYY240 pKa = 10.58 SIARR244 pKa = 11.84 KK245 pKa = 8.95 MSGEE249 pKa = 4.09 SDD251 pKa = 3.5 TALSNALDD259 pKa = 3.67 NWVTWLFLLGKK270 pKa = 10.04 KK271 pKa = 9.29 GVPPHH276 pKa = 5.88 QAAEE280 pKa = 4.11 MIYY283 pKa = 10.78 VEE285 pKa = 4.69 GDD287 pKa = 3.53 DD288 pKa = 4.98 NASSLQNHH296 pKa = 6.67 KK297 pKa = 10.44 LDD299 pKa = 4.13 KK300 pKa = 10.86 KK301 pKa = 10.81 DD302 pKa = 4.77 FEE304 pKa = 4.99 DD305 pKa = 4.4 LGLKK309 pKa = 9.96 IKK311 pKa = 10.55 IEE313 pKa = 4.0 TGLDD317 pKa = 2.98 IEE319 pKa = 4.5 QTGFCQLYY327 pKa = 8.09 FTLPPTGTICADD339 pKa = 3.09 PWKK342 pKa = 10.63 KK343 pKa = 10.08 LVKK346 pKa = 10.4 FSKK349 pKa = 10.85 APVKK353 pKa = 10.21 YY354 pKa = 9.96 AQASDD359 pKa = 3.58 KK360 pKa = 11.0 VLNSLLRR367 pKa = 11.84 AQALSMLYY375 pKa = 9.51 LHH377 pKa = 6.95 KK378 pKa = 10.49 GAPVVHH384 pKa = 6.77 KK385 pKa = 10.38 LAEE388 pKa = 4.05 RR389 pKa = 11.84 LLYY392 pKa = 8.52 ITRR395 pKa = 11.84 GFNVRR400 pKa = 11.84 NHH402 pKa = 5.52 HH403 pKa = 6.48 WEE405 pKa = 3.8 SAMKK409 pKa = 10.66 YY410 pKa = 10.2 GFDD413 pKa = 3.59 GNLIKK418 pKa = 9.99 TMKK421 pKa = 8.47 WRR423 pKa = 11.84 DD424 pKa = 3.7 YY425 pKa = 10.87 IDD427 pKa = 3.9 TPVKK431 pKa = 10.19 MEE433 pKa = 4.15 DD434 pKa = 3.07 RR435 pKa = 11.84 VLVEE439 pKa = 4.21 RR440 pKa = 11.84 QFGMTIEE447 pKa = 4.03 MQLLIEE453 pKa = 4.74 EE454 pKa = 5.19 KK455 pKa = 10.45 LDD457 pKa = 3.42 SWKK460 pKa = 11.03 GGLLHH465 pKa = 7.1 LPVDD469 pKa = 4.29 WFPDD473 pKa = 3.31 DD474 pKa = 3.18 WALFYY479 pKa = 11.14 SLYY482 pKa = 10.32 ATDD485 pKa = 4.99 GVHH488 pKa = 6.91 SSWFSPEE495 pKa = 3.17 GTLRR499 pKa = 11.84 KK500 pKa = 8.89 HH501 pKa = 6.43 HH502 pKa = 7.33 KK503 pKa = 9.5 DD504 pKa = 3.06 TILSFTRR511 pKa = 11.84 KK512 pKa = 7.48 TKK514 pKa = 10.66 SGTNVLFRR522 pKa = 11.84 PARR525 pKa = 11.84 SPGATKK531 pKa = 10.92 YY532 pKa = 10.62 KK533 pKa = 10.6 GFNNKK538 pKa = 9.07 LSQGNN543 pKa = 3.48
Molecular weight: 63.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.534
IPC2_protein 8.463
IPC_protein 8.317
Toseland 9.063
ProMoST 8.873
Dawson 9.37
Bjellqvist 9.136
Wikipedia 9.516
Rodwell 9.633
Grimsley 9.414
Solomon 9.399
Lehninger 9.37
Nozaki 9.253
DTASelect 9.077
Thurlkill 9.224
EMBOSS 9.516
Sillero 9.355
Patrickios 4.774
IPC_peptide 9.399
IPC2_peptide 7.673
IPC2.peptide.svr19 7.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1555
543
1012
777.5
88.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.688 ± 0.865
1.35 ± 0.139
6.109 ± 0.19
5.852 ± 0.335
5.145 ± 0.111
6.045 ± 0.19
2.444 ± 0.492
4.952 ± 0.196
7.524 ± 1.575
7.524 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.151 ± 0.716
4.695 ± 0.363
5.08 ± 0.477
3.215 ± 0.464
5.402 ± 0.139
5.723 ± 0.216
6.881 ± 0.558
6.817 ± 0.937
1.736 ± 0.475
3.601 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here