Eggerthellaceae bacterium zg-1050

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; unclassified Eggerthellaceae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1403 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6M8IZR0|A0A6M8IZR0_9ACTN Ribonuclease 3 OS=Eggerthellaceae bacterium zg-1050 OX=2734634 GN=rnc PE=3 SV=1
MM1 pKa = 7.33AAALIKK7 pKa = 10.76LPAEE11 pKa = 4.31LFALAEE17 pKa = 4.3GSRR20 pKa = 11.84FEE22 pKa = 4.14GRR24 pKa = 11.84LDD26 pKa = 3.54VGEE29 pKa = 4.75LRR31 pKa = 11.84LGPDD35 pKa = 3.69SYY37 pKa = 11.66RR38 pKa = 11.84LDD40 pKa = 3.88EE41 pKa = 4.48PVTWEE46 pKa = 4.2ADD48 pKa = 3.45VTNTGEE54 pKa = 4.05AFLVAGVAHH63 pKa = 6.27ATARR67 pKa = 11.84TEE69 pKa = 4.14CARR72 pKa = 11.84CLSTVEE78 pKa = 4.16EE79 pKa = 4.63SLTGSIEE86 pKa = 4.44GYY88 pKa = 10.29FLIDD92 pKa = 3.31PEE94 pKa = 4.14QRR96 pKa = 11.84TVDD99 pKa = 3.45GMEE102 pKa = 3.9GDD104 pKa = 3.94EE105 pKa = 5.34FDD107 pKa = 4.43VLPANHH113 pKa = 6.5QIDD116 pKa = 4.75LAPLITAGLLMEE128 pKa = 4.93LPGVPLCSEE137 pKa = 4.57DD138 pKa = 4.34CAGLCPSCGADD149 pKa = 4.15LNEE152 pKa = 4.92GPCACGGDD160 pKa = 3.75PALDD164 pKa = 4.23DD165 pKa = 4.32FAQAAHH171 pKa = 7.2PFAALRR177 pKa = 11.84GLSLAGDD184 pKa = 4.23AQPGDD189 pKa = 3.66TAVDD193 pKa = 3.52EE194 pKa = 4.79GSGGNGGGGASS205 pKa = 3.41

Molecular weight:
20.95 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6M8J326|A0A6M8J326_9ACTN Response regulator transcription factor OS=Eggerthellaceae bacterium zg-1050 OX=2734634 GN=HLV38_05445 PE=4 SV=1
MM1 pKa = 7.5NIGRR5 pKa = 11.84AVRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84AAEE13 pKa = 4.11LGLSQAEE20 pKa = 4.29LCLTTGISNAYY31 pKa = 9.45MSMLMNSKK39 pKa = 9.98IADD42 pKa = 3.55PRR44 pKa = 11.84ISRR47 pKa = 11.84VIQVAEE53 pKa = 4.1VLDD56 pKa = 3.88LTIDD60 pKa = 3.7EE61 pKa = 4.42LVQRR65 pKa = 11.84AEE67 pKa = 4.39AEE69 pKa = 4.12SSVTSRR75 pKa = 11.84VRR77 pKa = 11.84KK78 pKa = 9.95SEE80 pKa = 3.82GGGLTGKK87 pKa = 9.92HH88 pKa = 5.68PASARR93 pKa = 11.84WSKK96 pKa = 11.3APAPAVTPLWAGGEE110 pKa = 3.88ARR112 pKa = 11.84ARR114 pKa = 11.84ATRR117 pKa = 11.84SCVGRR122 pKa = 11.84APAAA126 pKa = 3.69

Molecular weight:
13.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1403

0

1403

508505

37

2328

362.4

39.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.186 ± 0.108

1.499 ± 0.032

5.898 ± 0.053

6.061 ± 0.06

3.481 ± 0.033

8.677 ± 0.055

1.869 ± 0.026

4.296 ± 0.048

3.05 ± 0.06

9.331 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.03

2.464 ± 0.035

4.623 ± 0.05

3.165 ± 0.037

7.033 ± 0.064

5.837 ± 0.055

5.059 ± 0.05

8.502 ± 0.065

0.99 ± 0.021

2.45 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski