Eggerthellaceae bacterium zg-1050
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1403 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M8IZR0|A0A6M8IZR0_9ACTN Ribonuclease 3 OS=Eggerthellaceae bacterium zg-1050 OX=2734634 GN=rnc PE=3 SV=1
MM1 pKa = 7.33 AAALIKK7 pKa = 10.76 LPAEE11 pKa = 4.31 LFALAEE17 pKa = 4.3 GSRR20 pKa = 11.84 FEE22 pKa = 4.14 GRR24 pKa = 11.84 LDD26 pKa = 3.54 VGEE29 pKa = 4.75 LRR31 pKa = 11.84 LGPDD35 pKa = 3.69 SYY37 pKa = 11.66 RR38 pKa = 11.84 LDD40 pKa = 3.88 EE41 pKa = 4.48 PVTWEE46 pKa = 4.2 ADD48 pKa = 3.45 VTNTGEE54 pKa = 4.05 AFLVAGVAHH63 pKa = 6.27 ATARR67 pKa = 11.84 TEE69 pKa = 4.14 CARR72 pKa = 11.84 CLSTVEE78 pKa = 4.16 EE79 pKa = 4.63 SLTGSIEE86 pKa = 4.44 GYY88 pKa = 10.29 FLIDD92 pKa = 3.31 PEE94 pKa = 4.14 QRR96 pKa = 11.84 TVDD99 pKa = 3.45 GMEE102 pKa = 3.9 GDD104 pKa = 3.94 EE105 pKa = 5.34 FDD107 pKa = 4.43 VLPANHH113 pKa = 6.5 QIDD116 pKa = 4.75 LAPLITAGLLMEE128 pKa = 4.93 LPGVPLCSEE137 pKa = 4.57 DD138 pKa = 4.34 CAGLCPSCGADD149 pKa = 4.15 LNEE152 pKa = 4.92 GPCACGGDD160 pKa = 3.75 PALDD164 pKa = 4.23 DD165 pKa = 4.32 FAQAAHH171 pKa = 7.2 PFAALRR177 pKa = 11.84 GLSLAGDD184 pKa = 4.23 AQPGDD189 pKa = 3.66 TAVDD193 pKa = 3.52 EE194 pKa = 4.79 GSGGNGGGGASS205 pKa = 3.41
Molecular weight: 20.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.643
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.126
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A6M8J326|A0A6M8J326_9ACTN Response regulator transcription factor OS=Eggerthellaceae bacterium zg-1050 OX=2734634 GN=HLV38_05445 PE=4 SV=1
MM1 pKa = 7.5 NIGRR5 pKa = 11.84 AVRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 AAEE13 pKa = 4.11 LGLSQAEE20 pKa = 4.29 LCLTTGISNAYY31 pKa = 9.45 MSMLMNSKK39 pKa = 9.98 IADD42 pKa = 3.55 PRR44 pKa = 11.84 ISRR47 pKa = 11.84 VIQVAEE53 pKa = 4.1 VLDD56 pKa = 3.88 LTIDD60 pKa = 3.7 EE61 pKa = 4.42 LVQRR65 pKa = 11.84 AEE67 pKa = 4.39 AEE69 pKa = 4.12 SSVTSRR75 pKa = 11.84 VRR77 pKa = 11.84 KK78 pKa = 9.95 SEE80 pKa = 3.82 GGGLTGKK87 pKa = 9.92 HH88 pKa = 5.68 PASARR93 pKa = 11.84 WSKK96 pKa = 11.3 APAPAVTPLWAGGEE110 pKa = 3.88 ARR112 pKa = 11.84 ARR114 pKa = 11.84 ATRR117 pKa = 11.84 SCVGRR122 pKa = 11.84 APAAA126 pKa = 3.69
Molecular weight: 13.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.385
IPC_protein 10.116
Toseland 10.657
ProMoST 10.365
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.833
Grimsley 10.76
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.067
Sillero 10.672
Patrickios 10.628
IPC_peptide 10.906
IPC2_peptide 9.633
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1403
0
1403
508505
37
2328
362.4
39.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.186 ± 0.108
1.499 ± 0.032
5.898 ± 0.053
6.061 ± 0.06
3.481 ± 0.033
8.677 ± 0.055
1.869 ± 0.026
4.296 ± 0.048
3.05 ± 0.06
9.331 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.03
2.464 ± 0.035
4.623 ± 0.05
3.165 ± 0.037
7.033 ± 0.064
5.837 ± 0.055
5.059 ± 0.05
8.502 ± 0.065
0.99 ± 0.021
2.45 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here