Candidatus Frackibacter sp. WG12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halobacteroidaceae; Candidatus Frackibacter; unclassified Candidatus Frackibacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8CN64|A0A1H8CN64_9FIRM Cysteine synthase OS=Candidatus Frackibacter sp. WG12 OX=2017977 GN=SAMN04488698_12815 PE=3 SV=1
MM1 pKa = 7.86PKK3 pKa = 10.51GDD5 pKa = 4.3LALKK9 pKa = 9.99VDD11 pKa = 4.28PEE13 pKa = 4.29ACISCGLCIEE23 pKa = 4.69TCPSVFEE30 pKa = 4.19WDD32 pKa = 3.94DD33 pKa = 3.63NEE35 pKa = 4.17KK36 pKa = 9.81ATAIVDD42 pKa = 3.82TVPEE46 pKa = 4.29KK47 pKa = 11.39VEE49 pKa = 3.9GDD51 pKa = 3.5AIEE54 pKa = 4.38AQEE57 pKa = 4.56GCPTDD62 pKa = 5.05AIVDD66 pKa = 3.69AA67 pKa = 5.37

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7YJH9|A0A1H7YJH9_9FIRM Benzoyl-CoA reductase/2-hydroxyglutaryl-CoA dehydratase subunit BcrC/BadD/HgdB OS=Candidatus Frackibacter sp. WG12 OX=2017977 GN=SAMN04488698_104126 PE=3 SV=1
MM1 pKa = 8.03SEE3 pKa = 4.3IILIQRR9 pKa = 11.84GRR11 pKa = 11.84IEE13 pKa = 4.0LFDD16 pKa = 5.03RR17 pKa = 11.84FFSWLYY23 pKa = 10.08ARR25 pKa = 11.84TRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.5SDD31 pKa = 3.07KK32 pKa = 9.16VDD34 pKa = 2.81KK35 pKa = 10.83YY36 pKa = 11.31GAIGLILFTAIPLPTTGAWTASVAATIFKK65 pKa = 10.31IRR67 pKa = 11.84FRR69 pKa = 11.84SAFPAIITGVLIAGVVITLLSTGVSHH95 pKa = 7.66FF96 pKa = 3.88

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2687

0

2687

804300

26

1556

299.3

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.475 ± 0.058

0.872 ± 0.023

5.831 ± 0.043

8.53 ± 0.063

3.783 ± 0.035

6.828 ± 0.045

1.481 ± 0.021

8.576 ± 0.048

8.021 ± 0.052

10.191 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.022

5.159 ± 0.044

3.202 ± 0.026

3.352 ± 0.024

4.03 ± 0.033

5.416 ± 0.032

4.835 ± 0.03

7.012 ± 0.047

0.712 ± 0.014

3.447 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski