Clostridiaceae bacterium JG1575
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2035 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I0N2U5|A0A2I0N2U5_9CLOT Methionine aminopeptidase OS=Clostridiaceae bacterium JG1575 OX=1658742 GN=map PE=3 SV=1
MM1 pKa = 8.25 SEE3 pKa = 3.96 EE4 pKa = 4.08 KK5 pKa = 10.77 KK6 pKa = 9.49 FTEE9 pKa = 3.89 EE10 pKa = 3.53 AKK12 pKa = 10.89 AEE14 pKa = 4.09 EE15 pKa = 4.87 SCCSHH20 pKa = 6.78 EE21 pKa = 4.45 EE22 pKa = 3.71 AHH24 pKa = 7.3 KK25 pKa = 10.4 DD26 pKa = 3.53 HH27 pKa = 7.16 CCGEE31 pKa = 4.5 HH32 pKa = 5.7 KK33 pKa = 10.24 HH34 pKa = 5.53 EE35 pKa = 5.93 HH36 pKa = 6.81 DD37 pKa = 4.28 GEE39 pKa = 4.56 CCGEE43 pKa = 4.26 HH44 pKa = 5.63 KK45 pKa = 10.3 HH46 pKa = 5.25 EE47 pKa = 5.87 HH48 pKa = 6.21 NGEE51 pKa = 3.92 CCKK54 pKa = 10.68 NKK56 pKa = 10.36 KK57 pKa = 8.36 EE58 pKa = 4.08 AEE60 pKa = 4.05 EE61 pKa = 3.94 AAEE64 pKa = 4.33 EE65 pKa = 4.27 VTLDD69 pKa = 3.85 EE70 pKa = 5.49 DD71 pKa = 4.11 LEE73 pKa = 4.44 EE74 pKa = 6.41 DD75 pKa = 5.18 GEE77 pKa = 4.47 EE78 pKa = 4.09 EE79 pKa = 3.79 DD80 pKa = 3.83 HH81 pKa = 6.58 GAVVVMEE88 pKa = 4.68 NEE90 pKa = 4.26 DD91 pKa = 3.8 GTTEE95 pKa = 4.15 EE96 pKa = 4.78 YY97 pKa = 10.44 PVVDD101 pKa = 3.66 EE102 pKa = 4.88 FEE104 pKa = 4.63 YY105 pKa = 11.15 DD106 pKa = 3.11 GSVYY110 pKa = 10.95 VLVEE114 pKa = 4.09 NADD117 pKa = 3.7 GTVTPLRR124 pKa = 11.84 ADD126 pKa = 3.74 GEE128 pKa = 4.37 DD129 pKa = 3.74 GEE131 pKa = 5.08 LAFLSEE137 pKa = 4.4 EE138 pKa = 4.06 EE139 pKa = 4.01 FAEE142 pKa = 4.33 VSNAYY147 pKa = 10.49 NEE149 pKa = 4.61 FEE151 pKa = 4.56 DD152 pKa = 5.31 AYY154 pKa = 11.27 SDD156 pKa = 3.77 EE157 pKa = 5.79 DD158 pKa = 4.14 DD159 pKa = 3.89 MEE161 pKa = 4.59 LVEE164 pKa = 5.28 EE165 pKa = 5.25 DD166 pKa = 3.29 IEE168 pKa = 4.78 IDD170 pKa = 3.55 IEE172 pKa = 4.31 EE173 pKa = 4.39 KK174 pKa = 10.94
Molecular weight: 19.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.757
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.706
Rodwell 3.757
Grimsley 3.668
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.075
Thurlkill 3.757
EMBOSS 3.732
Sillero 4.024
Patrickios 1.303
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A2I0N1K5|A0A2I0N1K5_9CLOT Uncharacterized protein OS=Clostridiaceae bacterium JG1575 OX=1658742 GN=ABB02_00688 PE=4 SV=1
MM1 pKa = 7.32 ARR3 pKa = 11.84 EE4 pKa = 3.72 ISPNKK9 pKa = 9.67 EE10 pKa = 3.34 NSTEE14 pKa = 4.07 SRR16 pKa = 11.84 SGGQRR21 pKa = 11.84 EE22 pKa = 3.85 EE23 pKa = 3.69 RR24 pKa = 11.84 RR25 pKa = 11.84 EE26 pKa = 3.75 YY27 pKa = 10.67 RR28 pKa = 11.84 RR29 pKa = 11.84 TNPRR33 pKa = 11.84 VRR35 pKa = 11.84 RR36 pKa = 11.84 SRR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 8.51 VCQFCVDD47 pKa = 3.45 HH48 pKa = 7.15 VDD50 pKa = 3.81 TIDD53 pKa = 3.6 YY54 pKa = 10.86 KK55 pKa = 11.14 DD56 pKa = 3.51 VPKK59 pKa = 10.4 LRR61 pKa = 11.84 KK62 pKa = 9.93 YY63 pKa = 8.24 ITEE66 pKa = 4.01 RR67 pKa = 11.84 GKK69 pKa = 10.04 ILPRR73 pKa = 11.84 RR74 pKa = 11.84 ISGTCAKK81 pKa = 9.14 HH82 pKa = 5.58 QRR84 pKa = 11.84 VLTEE88 pKa = 4.42 AIKK91 pKa = 10.29 RR92 pKa = 11.84 ARR94 pKa = 11.84 NIALLPFTNDD104 pKa = 2.6
Molecular weight: 12.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.677
IPC_protein 10.628
Toseland 10.877
ProMoST 10.687
Dawson 10.935
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.067
Grimsley 10.979
Solomon 11.14
Lehninger 11.096
Nozaki 10.862
DTASelect 10.687
Thurlkill 10.862
EMBOSS 11.301
Sillero 10.891
Patrickios 10.804
IPC_peptide 11.14
IPC2_peptide 9.882
IPC2.peptide.svr19 8.666
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2035
0
2035
668580
30
2118
328.5
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.732 ± 0.046
1.014 ± 0.018
5.025 ± 0.038
7.129 ± 0.063
4.09 ± 0.036
7.434 ± 0.046
2.096 ± 0.024
5.796 ± 0.048
5.755 ± 0.049
11.202 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.702 ± 0.024
3.267 ± 0.036
4.528 ± 0.039
3.806 ± 0.031
5.75 ± 0.047
5.714 ± 0.035
5.172 ± 0.041
6.744 ± 0.043
0.951 ± 0.019
3.094 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here