Amino acid dipepetide frequency for Clostridiaceae bacterium JG1575

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.217AlaAla: 8.217 ± 0.127	0.936AlaCys: 0.936 ± 0.042	3.769AlaAsp: 3.769 ± 0.086	3.955AlaGlu: 3.955 ± 0.083	3.696AlaPhe: 3.696 ± 0.082	5.998AlaGly: 5.998 ± 0.096	1.944AlaHis: 1.944 ± 0.057	4.821AlaIle: 4.821 ± 0.093	5.567AlaLys: 5.567 ± 0.119	11.818AlaLeu: 11.818 ± 0.163	2.655AlaMet: 2.655 ± 0.068	2.263AlaAsn: 2.263 ± 0.059	4.152AlaPro: 4.152 ± 0.094	4.631AlaGln: 4.631 ± 0.092	4.854AlaArg: 4.854 ± 0.1	4.749AlaSer: 4.749 ± 0.086	3.866AlaThr: 3.866 ± 0.076	5.832AlaVal: 5.832 ± 0.097	0.754AlaTrp: 0.754 ± 0.032	2.84AlaTyr: 2.84 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	0.95CysAla: 0.95 ± 0.042	0.123CysCys: 0.123 ± 0.016	0.583CysAsp: 0.583 ± 0.035	0.588CysGlu: 0.588 ± 0.03	0.386CysPhe: 0.386 ± 0.025	1.013CysGly: 1.013 ± 0.05	0.289CysHis: 0.289 ± 0.023	0.601CysIle: 0.601 ± 0.031	0.407CysLys: 0.407 ± 0.026	1.062CysLeu: 1.062 ± 0.046	0.205CysMet: 0.205 ± 0.018	0.292CysAsn: 0.292 ± 0.019	0.583CysPro: 0.583 ± 0.036	0.39CysGln: 0.39 ± 0.026	0.509CysArg: 0.509 ± 0.03	0.622CysSer: 0.622 ± 0.033	0.507CysThr: 0.507 ± 0.028	0.672CysVal: 0.672 ± 0.034	0.061CysTrp: 0.061 ± 0.009	0.301CysTyr: 0.301 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.535AspAla: 4.535 ± 0.085	0.452AspCys: 0.452 ± 0.025	1.982AspAsp: 1.982 ± 0.062	3.962AspGlu: 3.962 ± 0.088	2.345AspPhe: 2.345 ± 0.068	3.373AspGly: 3.373 ± 0.084	1.223AspHis: 1.223 ± 0.05	2.83AspIle: 2.83 ± 0.085	2.004AspLys: 2.004 ± 0.069	6.508AspLeu: 6.508 ± 0.094	1.022AspMet: 1.022 ± 0.041	1.229AspAsn: 1.229 ± 0.046	3.261AspPro: 3.261 ± 0.078	2.06AspGln: 2.06 ± 0.053	3.009AspArg: 3.009 ± 0.072	2.29AspSer: 2.29 ± 0.06	2.103AspThr: 2.103 ± 0.061	3.584AspVal: 3.584 ± 0.079	0.553AspTrp: 0.553 ± 0.029	1.928AspTyr: 1.928 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	7.504GluAla: 7.504 ± 0.122	0.476GluCys: 0.476 ± 0.028	3.913GluAsp: 3.913 ± 0.081	7.179GluGlu: 7.179 ± 0.132	2.193GluPhe: 2.193 ± 0.051	5.628GluGly: 5.628 ± 0.083	1.4GluHis: 1.4 ± 0.044	3.94GluIle: 3.94 ± 0.09	4.74GluLys: 4.74 ± 0.092	6.544GluLeu: 6.544 ± 0.1	1.906GluMet: 1.906 ± 0.05	2.425GluAsn: 2.425 ± 0.064	2.362GluPro: 2.362 ± 0.062	2.338GluGln: 2.338 ± 0.05	4.526GluArg: 4.526 ± 0.093	3.291GluSer: 3.291 ± 0.064	3.379GluThr: 3.379 ± 0.082	5.148GluVal: 5.148 ± 0.09	0.594GluTrp: 0.594 ± 0.03	1.802GluTyr: 1.802 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.205PheAla: 3.205 ± 0.075	0.576PheCys: 0.576 ± 0.028	2.136PheAsp: 2.136 ± 0.066	2.426PheGlu: 2.426 ± 0.069	2.335PhePhe: 2.335 ± 0.072	2.967PheGly: 2.967 ± 0.071	0.821PheHis: 0.821 ± 0.039	2.499PheIle: 2.499 ± 0.06	1.587PheLys: 1.587 ± 0.053	5.078PheLeu: 5.078 ± 0.114	1.069PheMet: 1.069 ± 0.042	1.297PheAsn: 1.297 ± 0.048	1.663PhePro: 1.663 ± 0.053	1.69PheGln: 1.69 ± 0.054	1.997PheArg: 1.997 ± 0.052	2.914PheSer: 2.914 ± 0.074	2.133PheThr: 2.133 ± 0.057	2.58PheVal: 2.58 ± 0.073	0.467PheTrp: 0.467 ± 0.029	1.454PheTyr: 1.454 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	6.704GlyAla: 6.704 ± 0.12	0.888GlyCys: 0.888 ± 0.046	3.513GlyAsp: 3.513 ± 0.065	4.927GlyGlu: 4.927 ± 0.094	3.344GlyPhe: 3.344 ± 0.068	5.233GlyGly: 5.233 ± 0.103	1.499GlyHis: 1.499 ± 0.049	5.105GlyIle: 5.105 ± 0.095	4.285GlyLys: 4.285 ± 0.092	7.298GlyLeu: 7.298 ± 0.136	2.078GlyMet: 2.078 ± 0.063	2.212GlyAsn: 2.212 ± 0.068	2.665GlyPro: 2.665 ± 0.063	2.408GlyGln: 2.408 ± 0.07	4.364GlyArg: 4.364 ± 0.083	4.381GlySer: 4.381 ± 0.078	4.484GlyThr: 4.484 ± 0.103	5.586GlyVal: 5.586 ± 0.102	0.718GlyTrp: 0.718 ± 0.035	2.65GlyTyr: 2.65 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.594HisAla: 1.594 ± 0.052	0.322HisCys: 0.322 ± 0.023	0.862HisAsp: 0.862 ± 0.038	1.422HisGlu: 1.422 ± 0.046	0.963HisPhe: 0.963 ± 0.037	1.584HisGly: 1.584 ± 0.054	0.646HisHis: 0.646 ± 0.032	0.981HisIle: 0.981 ± 0.042	0.827HisLys: 0.827 ± 0.034	2.615HisLeu: 2.615 ± 0.068	0.498HisMet: 0.498 ± 0.029	0.498HisAsn: 0.498 ± 0.026	1.401HisPro: 1.401 ± 0.042	0.915HisGln: 0.915 ± 0.039	1.494HisArg: 1.494 ± 0.047	1.111HisSer: 1.111 ± 0.041	0.938HisThr: 0.938 ± 0.042	1.247HisVal: 1.247 ± 0.043	0.293HisTrp: 0.293 ± 0.022	0.749HisTyr: 0.749 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.082IleAla: 5.082 ± 0.094	0.588IleCys: 0.588 ± 0.032	2.763IleAsp: 2.763 ± 0.069	3.641IleGlu: 3.641 ± 0.081	2.309IlePhe: 2.309 ± 0.071	3.819IleGly: 3.819 ± 0.078	1.445IleHis: 1.445 ± 0.05	3.264IleIle: 3.264 ± 0.085	2.812IleLys: 2.812 ± 0.078	7.278IleLeu: 7.278 ± 0.12	1.369IleMet: 1.369 ± 0.043	2.124IleAsn: 2.124 ± 0.059	3.074IlePro: 3.074 ± 0.074	2.3IleGln: 2.3 ± 0.059	3.578IleArg: 3.578 ± 0.076	3.469IleSer: 3.469 ± 0.073	3.258IleThr: 3.258 ± 0.071	3.612IleVal: 3.612 ± 0.092	0.419IleTrp: 0.419 ± 0.026	1.754IleTyr: 1.754 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.162LysAla: 5.162 ± 0.109	0.286LysCys: 0.286 ± 0.021	4.019LysAsp: 4.019 ± 0.086	6.33LysGlu: 6.33 ± 0.104	1.276LysPhe: 1.276 ± 0.052	4.605LysGly: 4.605 ± 0.082	0.847LysHis: 0.847 ± 0.035	3.148LysIle: 3.148 ± 0.075	4.833LysLys: 4.833 ± 0.108	4.007LysLeu: 4.007 ± 0.084	1.422LysMet: 1.422 ± 0.048	2.682LysAsn: 2.682 ± 0.065	2.107LysPro: 2.107 ± 0.066	1.629LysGln: 1.629 ± 0.047	2.99LysArg: 2.99 ± 0.065	2.935LysSer: 2.935 ± 0.066	3.676LysThr: 3.676 ± 0.074	3.842LysVal: 3.842 ± 0.097	0.378LysTrp: 0.378 ± 0.028	1.379LysTyr: 1.379 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	9.917LeuAla: 9.917 ± 0.142	1.385LeuCys: 1.385 ± 0.05	5.808LeuAsp: 5.808 ± 0.091	7.28LeuGlu: 7.28 ± 0.112	4.794LeuPhe: 4.794 ± 0.11	8.286LeuGly: 8.286 ± 0.133	2.01LeuHis: 2.01 ± 0.06	6.183LeuIle: 6.183 ± 0.104	6.556LeuLys: 6.556 ± 0.102	12.821LeuLeu: 12.821 ± 0.222	3.162LeuMet: 3.162 ± 0.067	3.648LeuAsn: 3.648 ± 0.072	5.135LeuPro: 5.135 ± 0.115	3.801LeuGln: 3.801 ± 0.087	6.505LeuArg: 6.505 ± 0.119	7.677LeuSer: 7.677 ± 0.127	6.043LeuThr: 6.043 ± 0.104	6.659LeuVal: 6.659 ± 0.106	1.234LeuTrp: 1.234 ± 0.052	3.117LeuTyr: 3.117 ± 0.073	0.001LeuXaa: 0.001 ± 0.002
Met	2.707MetAla: 2.707 ± 0.059	0.145MetCys: 0.145 ± 0.015	1.91MetAsp: 1.91 ± 0.058	2.112MetGlu: 2.112 ± 0.059	0.598MetPhe: 0.598 ± 0.032	2.251MetGly: 2.251 ± 0.059	0.458MetHis: 0.458 ± 0.026	1.596MetIle: 1.596 ± 0.056	2.079MetLys: 2.079 ± 0.056	2.076MetLeu: 2.076 ± 0.055	0.764MetMet: 0.764 ± 0.031	1.461MetAsn: 1.461 ± 0.051	1.099MetPro: 1.099 ± 0.037	0.808MetGln: 0.808 ± 0.035	1.384MetArg: 1.384 ± 0.049	1.446MetSer: 1.446 ± 0.04	1.554MetThr: 1.554 ± 0.047	1.935MetVal: 1.935 ± 0.052	0.124MetTrp: 0.124 ± 0.014	0.513MetTyr: 0.513 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	2.778AsnAla: 2.778 ± 0.072	0.289AsnCys: 0.289 ± 0.021	1.5AsnAsp: 1.5 ± 0.051	2.296AsnGlu: 2.296 ± 0.067	1.215AsnPhe: 1.215 ± 0.043	2.33AsnGly: 2.33 ± 0.063	0.823AsnHis: 0.823 ± 0.039	2.194AsnIle: 2.194 ± 0.06	1.639AsnLys: 1.639 ± 0.061	3.591AsnLeu: 3.591 ± 0.084	0.906AsnMet: 0.906 ± 0.036	1.161AsnAsn: 1.161 ± 0.045	2.063AsnPro: 2.063 ± 0.057	1.407AsnGln: 1.407 ± 0.047	1.9AsnArg: 1.9 ± 0.058	1.382AsnSer: 1.382 ± 0.052	1.602AsnThr: 1.602 ± 0.056	2.104AsnVal: 2.104 ± 0.055	0.313AsnTrp: 0.313 ± 0.023	1.176AsnTyr: 1.176 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	3.151ProAla: 3.151 ± 0.076	0.464ProCys: 0.464 ± 0.028	2.085ProAsp: 2.085 ± 0.062	3.86ProGlu: 3.86 ± 0.07	1.91ProPhe: 1.91 ± 0.057	3.329ProGly: 3.329 ± 0.07	1.032ProHis: 1.032 ± 0.039	2.395ProIle: 2.395 ± 0.052	3.128ProLys: 3.128 ± 0.077	5.247ProLeu: 5.247 ± 0.105	1.488ProMet: 1.488 ± 0.046	1.403ProAsn: 1.403 ± 0.047	1.436ProPro: 1.436 ± 0.056	2.155ProGln: 2.155 ± 0.057	2.1ProArg: 2.1 ± 0.064	2.851ProSer: 2.851 ± 0.069	2.172ProThr: 2.172 ± 0.059	3.062ProVal: 3.062 ± 0.08	0.523ProTrp: 0.523 ± 0.031	1.488ProTyr: 1.488 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	3.289GlnAla: 3.289 ± 0.081	0.304GlnCys: 0.304 ± 0.022	1.811GlnAsp: 1.811 ± 0.057	3.973GlnGlu: 3.973 ± 0.089	1.06GlnPhe: 1.06 ± 0.035	3.507GlnGly: 3.507 ± 0.083	0.564GlnHis: 0.564 ± 0.034	2.016GlnIle: 2.016 ± 0.058	3.195GlnLys: 3.195 ± 0.065	2.764GlnLeu: 2.764 ± 0.077	1.14GlnMet: 1.14 ± 0.039	1.385GlnAsn: 1.385 ± 0.048	1.188GlnPro: 1.188 ± 0.043	1.309GlnGln: 1.309 ± 0.05	2.574GlnArg: 2.574 ± 0.07	2.199GlnSer: 2.199 ± 0.061	1.949GlnThr: 1.949 ± 0.053	2.381GlnVal: 2.381 ± 0.064	0.474GlnTrp: 0.474 ± 0.026	0.975GlnTyr: 0.975 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	4.577ArgAla: 4.577 ± 0.108	0.606ArgCys: 0.606 ± 0.036	2.574ArgAsp: 2.574 ± 0.064	4.37ArgGlu: 4.37 ± 0.097	2.643ArgPhe: 2.643 ± 0.061	3.748ArgGly: 3.748 ± 0.08	1.174ArgHis: 1.174 ± 0.045	4.197ArgIle: 4.197 ± 0.083	2.959ArgLys: 2.959 ± 0.069	6.083ArgLeu: 6.083 ± 0.107	1.843ArgMet: 1.843 ± 0.055	1.84ArgAsn: 1.84 ± 0.058	2.378ArgPro: 2.378 ± 0.065	1.986ArgGln: 1.986 ± 0.059	3.86ArgArg: 3.86 ± 0.085	3.458ArgSer: 3.458 ± 0.083	2.848ArgThr: 2.848 ± 0.066	3.745ArgVal: 3.745 ± 0.071	0.672ArgTrp: 0.672 ± 0.029	1.941ArgTyr: 1.941 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	4.737SerAla: 4.737 ± 0.09	0.612SerCys: 0.612 ± 0.033	2.679SerAsp: 2.679 ± 0.064	3.301SerGlu: 3.301 ± 0.08	2.933SerPhe: 2.933 ± 0.076	4.83SerGly: 4.83 ± 0.085	1.111SerHis: 1.111 ± 0.042	3.41SerIle: 3.41 ± 0.082	3.0SerLys: 3.0 ± 0.072	7.432SerLeu: 7.432 ± 0.122	1.65SerMet: 1.65 ± 0.046	1.582SerAsn: 1.582 ± 0.052	2.725SerPro: 2.725 ± 0.064	1.98SerGln: 1.98 ± 0.058	2.908SerArg: 2.908 ± 0.065	3.335SerSer: 3.335 ± 0.073	2.612SerThr: 2.612 ± 0.058	3.91SerVal: 3.91 ± 0.083	0.594SerTrp: 0.594 ± 0.036	1.798SerTyr: 1.798 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.239ThrAla: 4.239 ± 0.081	0.474ThrCys: 0.474 ± 0.025	2.372ThrAsp: 2.372 ± 0.065	2.632ThrGlu: 2.632 ± 0.072	2.104ThrPhe: 2.104 ± 0.062	4.052ThrGly: 4.052 ± 0.089	1.225ThrHis: 1.225 ± 0.043	3.051ThrIle: 3.051 ± 0.076	2.982ThrLys: 2.982 ± 0.076	6.704ThrLeu: 6.704 ± 0.111	1.252ThrMet: 1.252 ± 0.042	1.57ThrAsn: 1.57 ± 0.052	3.093ThrPro: 3.093 ± 0.075	2.048ThrGln: 2.048 ± 0.057	2.363ThrArg: 2.363 ± 0.058	2.706ThrSer: 2.706 ± 0.072	2.804ThrThr: 2.804 ± 0.077	3.872ThrVal: 3.872 ± 0.076	0.429ThrTrp: 0.429 ± 0.025	1.741ThrTyr: 1.741 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	5.711ValAla: 5.711 ± 0.076	0.782ValCys: 0.782 ± 0.038	3.638ValAsp: 3.638 ± 0.075	4.055ValGlu: 4.055 ± 0.091	2.964ValPhe: 2.964 ± 0.067	4.64ValGly: 4.64 ± 0.082	1.455ValHis: 1.455 ± 0.045	3.581ValIle: 3.581 ± 0.082	3.166ValLys: 3.166 ± 0.089	8.711ValLeu: 8.711 ± 0.135	1.611ValMet: 1.611 ± 0.051	2.14ValAsn: 2.14 ± 0.058	3.192ValPro: 3.192 ± 0.083	2.442ValGln: 2.442 ± 0.069	3.961ValArg: 3.961 ± 0.076	4.154ValSer: 4.154 ± 0.082	3.635ValThr: 3.635 ± 0.085	5.13ValVal: 5.13 ± 0.097	0.526ValTrp: 0.526 ± 0.026	1.947ValTyr: 1.947 ± 0.063	0.0ValXaa: 0.0 ± 0.0
Trp	0.782TrpAla: 0.782 ± 0.03	0.081TrpCys: 0.081 ± 0.011	0.537TrpAsp: 0.537 ± 0.03	0.543TrpGlu: 0.543 ± 0.027	0.408TrpPhe: 0.408 ± 0.023	0.694TrpGly: 0.694 ± 0.034	0.215TrpHis: 0.215 ± 0.02	0.67TrpIle: 0.67 ± 0.031	0.443TrpLys: 0.443 ± 0.027	0.873TrpLeu: 0.873 ± 0.038	0.337TrpMet: 0.337 ± 0.023	0.378TrpAsn: 0.378 ± 0.023	0.396TrpPro: 0.396 ± 0.027	0.423TrpGln: 0.423 ± 0.025	0.585TrpArg: 0.585 ± 0.035	0.526TrpSer: 0.526 ± 0.029	0.607TrpThr: 0.607 ± 0.026	0.634TrpVal: 0.634 ± 0.029	0.078TrpTrp: 0.078 ± 0.011	0.293TrpTyr: 0.293 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.475TyrAla: 2.475 ± 0.06	0.356TyrCys: 0.356 ± 0.026	1.799TyrAsp: 1.799 ± 0.055	2.434TyrGlu: 2.434 ± 0.06	1.418TyrPhe: 1.418 ± 0.047	2.456TyrGly: 2.456 ± 0.053	0.781TyrHis: 0.781 ± 0.038	1.473TyrIle: 1.473 ± 0.052	1.345TyrLys: 1.345 ± 0.051	3.512TyrLeu: 3.512 ± 0.079	0.645TyrMet: 0.645 ± 0.033	1.077TyrAsn: 1.077 ± 0.043	1.306TyrPro: 1.306 ± 0.045	1.336TyrGln: 1.336 ± 0.055	2.043TyrArg: 2.043 ± 0.058	1.642TyrSer: 1.642 ± 0.052	1.547TyrThr: 1.547 ± 0.052	1.904TyrVal: 1.904 ± 0.059	0.301TyrTrp: 0.301 ± 0.021	1.092TyrTyr: 1.092 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2035 proteins (668580 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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