Alicyclobacillaceae bacterium I2511

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; unclassified Alicyclobacillaceae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3312 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418LW81|A0A418LW81_9BACL Acyl-CoA dehydrogenase OS=Alicyclobacillaceae bacterium I2511 OX=2048547 GN=D2Q93_15160 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 10.14KK3 pKa = 8.65VTPAKK8 pKa = 10.2VWMALGTSLLVPLTVPISAFAATSFDD34 pKa = 3.15SQYY37 pKa = 10.61QANVTQEE44 pKa = 3.77AALLQTAQSGTVTTAAGQSFSNQVQNLNSEE74 pKa = 4.04ISSLYY79 pKa = 9.6STEE82 pKa = 3.5QTLAQNSQSLSTSISNQTSQLQQLQTQRR110 pKa = 11.84KK111 pKa = 7.66QLLQEE116 pKa = 4.39SQVAWNQYY124 pKa = 8.2SRR126 pKa = 11.84YY127 pKa = 9.42IRR129 pKa = 11.84GNGRR133 pKa = 11.84NFRR136 pKa = 11.84GMAQSYY142 pKa = 9.22HH143 pKa = 5.12NQWLKK148 pKa = 10.75IGEE151 pKa = 4.25KK152 pKa = 9.81VLNVNGQLAGLNPSNQEE169 pKa = 3.86GNSEE173 pKa = 4.12YY174 pKa = 10.44QQGLSSLQNSILSLQSAEE192 pKa = 4.8IADD195 pKa = 3.88TQQWINLEE203 pKa = 4.07QNAGSGSIGSTISSTGLTAPTLVATTDD230 pKa = 3.48SNGNPEE236 pKa = 4.06IAVSNAQPGATVVLYY251 pKa = 10.57SSNGTQVSSTVADD264 pKa = 4.08DD265 pKa = 3.92NGNATFDD272 pKa = 3.58NVATGTYY279 pKa = 10.37YY280 pKa = 10.94GVQSLNGQQSGASGTVTITSISSVTSLSAPTIAEE314 pKa = 4.27TTDD317 pKa = 3.36SNGNPEE323 pKa = 4.12IVVSNAQPGATVVLYY338 pKa = 10.57SSNGTQVSSTVADD351 pKa = 4.08DD352 pKa = 3.92NGNATFDD359 pKa = 3.58NVATGTYY366 pKa = 10.39YY367 pKa = 10.94GVQNLNGQQSGASGTVTINGTSSLLITNANAQFDD401 pKa = 4.36GLLQTNITATVNLAVTRR418 pKa = 11.84VQIIDD423 pKa = 3.61EE424 pKa = 4.64TNGNTLSTATPSGGSLNVQIIGANSGDD451 pKa = 4.01TLTLIPYY458 pKa = 8.65EE459 pKa = 4.56GNTVEE464 pKa = 4.24QPVNVTVTEE473 pKa = 4.15NLPSTVLTAPTLVATTDD490 pKa = 3.48SNGNPEE496 pKa = 4.12IVVSNAQPGANLVLYY511 pKa = 10.35SSMGTVVNLTSADD524 pKa = 3.73DD525 pKa = 3.81NGNGVFDD532 pKa = 3.83NVANGTYY539 pKa = 10.57DD540 pKa = 2.99VVQIVNGQQSSASNSVTVAA559 pKa = 3.14

Molecular weight: 57.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418M3C4|A0A418M3C4_9BACL Uncharacterized protein OS=Alicyclobacillaceae bacterium I2511 OX=2048547 GN=D2Q93_11755 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 8.57PTYY5 pKa = 10.01QPNTRR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84KK13 pKa = 8.28KK14 pKa = 9.38VHH16 pKa = 5.93GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTKK25 pKa = 9.43NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3312

0

3312

970464

19

2434

293.0

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.177 ± 0.05

0.805 ± 0.015

4.507 ± 0.034

5.563 ± 0.052

3.792 ± 0.032

7.993 ± 0.043

2.532 ± 0.021

5.262 ± 0.037

3.6 ± 0.036

10.829 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.021

3.299 ± 0.03

4.606 ± 0.031

4.951 ± 0.041

5.697 ± 0.04

5.978 ± 0.036

5.812 ± 0.041

8.579 ± 0.041

1.582 ± 0.022

2.837 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski