Nitrosopumilaceae archaeon

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; Nitrosopumilaceae; unclassified Nitrosopumilaceae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A447G2S2|A0A447G2S2_9ARCH Uncharacterized protein OS=Nitrosopumilaceae archaeon OX=2202732 GN=IBTHAUMO2_190018 PE=4 SV=1
MM1 pKa = 6.76KK2 pKa = 9.23TAIIAGIVAAAAAVGVAAVLAGMGPDD28 pKa = 3.42QMDD31 pKa = 3.84GQMMADD37 pKa = 3.65EE38 pKa = 4.49MADD41 pKa = 3.28GGMMADD47 pKa = 3.98EE48 pKa = 4.35MAGGITVAASFLPIYY63 pKa = 10.13EE64 pKa = 4.09FATAVGGNTVSVSQLVPPGAEE85 pKa = 3.74PHH87 pKa = 6.59DD88 pKa = 3.89WEE90 pKa = 4.23PTVRR94 pKa = 11.84QVQEE98 pKa = 3.96LQEE101 pKa = 4.53ADD103 pKa = 3.18IIAINGIHH111 pKa = 5.91FEE113 pKa = 4.1EE114 pKa = 4.55WLGDD118 pKa = 3.37LDD120 pKa = 4.56AYY122 pKa = 10.65GGALVDD128 pKa = 3.63TSAPVEE134 pKa = 4.34VMHH137 pKa = 6.98EE138 pKa = 4.42DD139 pKa = 3.2PHH141 pKa = 7.71IWLVPTNAALQARR154 pKa = 11.84EE155 pKa = 3.66IAMALAEE162 pKa = 4.67IDD164 pKa = 3.73PGNADD169 pKa = 3.72EE170 pKa = 4.71YY171 pKa = 10.72MSNADD176 pKa = 3.13IFTAEE181 pKa = 4.21MDD183 pKa = 4.41ALDD186 pKa = 3.94ADD188 pKa = 3.53IRR190 pKa = 11.84AGLEE194 pKa = 3.93GCGTEE199 pKa = 4.57FVTAHH204 pKa = 6.26GAFSYY209 pKa = 10.31FAEE212 pKa = 4.7EE213 pKa = 4.23YY214 pKa = 11.07GLVQHH219 pKa = 7.12AIIAGGDD226 pKa = 3.49PHH228 pKa = 8.74GEE230 pKa = 3.95ASPHH234 pKa = 7.08DD235 pKa = 3.97IGDD238 pKa = 4.14AAAHH242 pKa = 5.84AAEE245 pKa = 4.32TGYY248 pKa = 10.51ILAEE252 pKa = 4.25SPSRR256 pKa = 11.84LVDD259 pKa = 3.38ATAAEE264 pKa = 4.4AGAEE268 pKa = 4.04VLILDD273 pKa = 4.38PLEE276 pKa = 4.41TSEE279 pKa = 5.41GPYY282 pKa = 9.78EE283 pKa = 4.07DD284 pKa = 3.29VMRR287 pKa = 11.84RR288 pKa = 11.84NLGVLQQALCTQQ300 pKa = 3.87

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A447G2F6|A0A447G2F6_9ARCH 30S ribosomal protein S4 OS=Nitrosopumilaceae archaeon OX=2202732 GN=rps4 PE=3 SV=1
MM1 pKa = 7.37MMMQRR6 pKa = 11.84TWRR9 pKa = 11.84RR10 pKa = 11.84PAPRR14 pKa = 11.84MAPRR18 pKa = 11.84RR19 pKa = 11.84VRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84NRR26 pKa = 11.84PRR28 pKa = 11.84QVTLRR33 pKa = 11.84PRR35 pKa = 11.84NRR37 pKa = 11.84PGQAARR43 pKa = 11.84QLRR46 pKa = 11.84NRR48 pKa = 11.84PRR50 pKa = 11.84QVTLRR55 pKa = 11.84PRR57 pKa = 11.84NRR59 pKa = 11.84PGQAARR65 pKa = 11.84QLRR68 pKa = 11.84NRR70 pKa = 11.84PRR72 pKa = 11.84RR73 pKa = 11.84PRR75 pKa = 11.84SRR77 pKa = 11.84HH78 pKa = 5.36RR79 pKa = 11.84PAQIPPGSRR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84QTAPPPEE97 pKa = 4.59GLPCAPP103 pKa = 4.65

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2237

0

2237

579143

20

5955

258.9

28.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.091 ± 0.094

1.268 ± 0.027

6.231 ± 0.069

6.332 ± 0.045

3.078 ± 0.033

10.251 ± 0.077

2.056 ± 0.024

5.152 ± 0.044

3.448 ± 0.072

8.242 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.935 ± 0.039

2.26 ± 0.04

5.292 ± 0.062

2.043 ± 0.029

7.978 ± 0.074

6.236 ± 0.039

4.72 ± 0.087

7.421 ± 0.056

1.025 ± 0.022

2.942 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski