Pseudogymnoascus sp. VKM F-4246

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093YUM7|A0A093YUM7_9PEZI Uncharacterized protein (Fragment) OS=Pseudogymnoascus sp. VKM F-4246 OX=1420902 GN=V492_05966 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84SSVLVLASVVGVALAQKK19 pKa = 10.75ASDD22 pKa = 3.87CSDD25 pKa = 3.41PQKK28 pKa = 11.24AEE30 pKa = 3.98SCVLKK35 pKa = 9.47LTKK38 pKa = 10.38AGLSCQTVLGIKK50 pKa = 9.54SCYY53 pKa = 10.36DD54 pKa = 3.43DD55 pKa = 4.34FCPEE59 pKa = 3.74IPLPDD64 pKa = 4.83EE65 pKa = 4.26INSIIDD71 pKa = 3.27TCEE74 pKa = 3.7NGGTFPGSGSGSGSGSGSGSGSGTGSGTGSEE105 pKa = 4.46SGTTTSSASQSTSSTNIPSDD125 pKa = 3.59CTDD128 pKa = 3.57ADD130 pKa = 3.9GFSDD134 pKa = 4.36CVKK137 pKa = 10.31PLVKK141 pKa = 10.24VDD143 pKa = 3.79TTCKK147 pKa = 9.37VTMQIQQCFKK157 pKa = 9.64TYY159 pKa = 10.16CPQHH163 pKa = 6.58IDD165 pKa = 3.8RR166 pKa = 11.84IEE168 pKa = 5.1DD169 pKa = 3.75DD170 pKa = 3.71VTSCQGSEE178 pKa = 4.25DD179 pKa = 3.85GSGTGSDD186 pKa = 4.55DD187 pKa = 4.52DD188 pKa = 4.09STPTSSSDD196 pKa = 3.27DD197 pKa = 3.78SEE199 pKa = 4.55GTGISGGGSSTDD211 pKa = 3.28TSSSADD217 pKa = 3.43STGTGVDD224 pKa = 3.26SGSDD228 pKa = 3.53NTSSSSDD235 pKa = 3.35PTNTSTNSDD244 pKa = 3.52PVATDD249 pKa = 3.32SNVNEE254 pKa = 4.29GAGDD258 pKa = 3.84KK259 pKa = 10.9LFAPAGAIIGSIIAVMAWLL278 pKa = 3.98

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093YLY4|A0A093YLY4_9PEZI Acylphosphatase OS=Pseudogymnoascus sp. VKM F-4246 OX=1420902 GN=V492_08180 PE=3 SV=1
MM1 pKa = 7.44VSKK4 pKa = 10.83LPRR7 pKa = 11.84NTTLLGSPSEE17 pKa = 4.17NNRR20 pKa = 11.84NLVIVLSAVFGSLALFMVVSATVFVYY46 pKa = 9.94RR47 pKa = 11.84WKK49 pKa = 10.77RR50 pKa = 11.84NPKK53 pKa = 6.65YY54 pKa = 9.72HH55 pKa = 6.87RR56 pKa = 11.84RR57 pKa = 11.84GISPVDD63 pKa = 3.43DD64 pKa = 4.09EE65 pKa = 5.41EE66 pKa = 4.64IEE68 pKa = 3.92RR69 pKa = 11.84WRR71 pKa = 11.84GRR73 pKa = 11.84KK74 pKa = 7.09EE75 pKa = 4.11TYY77 pKa = 9.7TEE79 pKa = 4.34APSRR83 pKa = 11.84TAPKK87 pKa = 7.38THH89 pKa = 6.54KK90 pKa = 9.33RR91 pKa = 11.84QSSSVVIVSNPPGWTWSAEE110 pKa = 3.67PSPFGTRR117 pKa = 11.84NSGEE121 pKa = 4.34TALSPPPMVARR132 pKa = 11.84APNSRR137 pKa = 11.84SGLTDD142 pKa = 3.5GAILGADD149 pKa = 3.35PFVPPVRR156 pKa = 11.84RR157 pKa = 11.84PSARR161 pKa = 11.84LAKK164 pKa = 10.15HH165 pKa = 6.01SRR167 pKa = 11.84NQSRR171 pKa = 11.84KK172 pKa = 8.11SSFSASIPEE181 pKa = 4.27RR182 pKa = 11.84MSTDD186 pKa = 3.27LPRR189 pKa = 11.84HH190 pKa = 5.45YY191 pKa = 10.65RR192 pKa = 11.84DD193 pKa = 3.7DD194 pKa = 3.9SKK196 pKa = 11.71KK197 pKa = 10.66SFQADD202 pKa = 3.53PVSPPSSIFNGSPGTNEE219 pKa = 4.23PLPTSDD225 pKa = 3.97TGFCRR230 pKa = 11.84LPSRR234 pKa = 11.84SIDD237 pKa = 3.61SLLGPLARR245 pKa = 11.84PLPNPPLHH253 pKa = 6.56QLDD256 pKa = 4.76AVDD259 pKa = 5.26APPPPPPLPHH269 pKa = 6.9PTQQPRR275 pKa = 11.84ARR277 pKa = 11.84LPRR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84IPRR285 pKa = 11.84RR286 pKa = 11.84AARR289 pKa = 11.84DD290 pKa = 4.13AIPLDD295 pKa = 3.78ARR297 pKa = 11.84DD298 pKa = 4.16GVHH301 pKa = 6.84AAHH304 pKa = 6.41GCVWGAAYY312 pKa = 10.18LWAGVLWRR320 pKa = 11.84DD321 pKa = 3.41CGVWNDD327 pKa = 3.67GVSEE331 pKa = 4.02GRR333 pKa = 11.84GEE335 pKa = 4.23DD336 pKa = 3.48GKK338 pKa = 11.23

Molecular weight: 37.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8539

0

8539

4315648

10

5650

505.4

55.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.947 ± 0.024

1.157 ± 0.01

5.761 ± 0.02

6.351 ± 0.026

3.608 ± 0.015

7.275 ± 0.028

2.216 ± 0.011

5.003 ± 0.018

5.081 ± 0.02

8.658 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.01

3.765 ± 0.013

5.915 ± 0.025

3.861 ± 0.019

5.784 ± 0.02

8.097 ± 0.026

6.016 ± 0.016

6.176 ± 0.018

1.407 ± 0.009

2.766 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski