Amino acid dipepetide frequency for Pseudogymnoascus sp. VKM F-4246

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.094AlaAla: 10.094 ± 0.081	1.031AlaCys: 1.031 ± 0.019	4.538AlaAsp: 4.538 ± 0.036	5.497AlaGlu: 5.497 ± 0.048	3.083AlaPhe: 3.083 ± 0.029	6.233AlaGly: 6.233 ± 0.042	1.732AlaHis: 1.732 ± 0.021	4.498AlaIle: 4.498 ± 0.04	4.505AlaLys: 4.505 ± 0.045	7.753AlaLeu: 7.753 ± 0.048	2.01AlaMet: 2.01 ± 0.024	3.156AlaAsn: 3.156 ± 0.032	5.236AlaPro: 5.236 ± 0.048	3.436AlaGln: 3.436 ± 0.031	4.822AlaArg: 4.822 ± 0.04	7.222AlaSer: 7.222 ± 0.04	5.629AlaThr: 5.629 ± 0.041	5.653AlaVal: 5.653 ± 0.044	1.144AlaTrp: 1.144 ± 0.019	2.2AlaTyr: 2.2 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	0.935CysAla: 0.935 ± 0.018	0.232CysCys: 0.232 ± 0.008	0.636CysAsp: 0.636 ± 0.013	0.577CysGlu: 0.577 ± 0.013	0.494CysPhe: 0.494 ± 0.011	0.968CysGly: 0.968 ± 0.02	0.294CysHis: 0.294 ± 0.01	0.668CysIle: 0.668 ± 0.014	0.492CysLys: 0.492 ± 0.012	1.131CysLeu: 1.131 ± 0.018	0.263CysMet: 0.263 ± 0.008	0.415CysAsn: 0.415 ± 0.012	0.61CysPro: 0.61 ± 0.015	0.404CysGln: 0.404 ± 0.01	0.668CysArg: 0.668 ± 0.014	0.85CysSer: 0.85 ± 0.016	0.673CysThr: 0.673 ± 0.015	0.73CysVal: 0.73 ± 0.016	0.189CysTrp: 0.189 ± 0.007	0.345CysTyr: 0.345 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.987AspAla: 4.987 ± 0.035	0.592AspCys: 0.592 ± 0.012	4.315AspAsp: 4.315 ± 0.051	4.715AspGlu: 4.715 ± 0.047	2.212AspPhe: 2.212 ± 0.023	4.375AspGly: 4.375 ± 0.036	1.12AspHis: 1.12 ± 0.017	3.225AspIle: 3.225 ± 0.031	2.516AspLys: 2.516 ± 0.03	4.907AspLeu: 4.907 ± 0.041	1.257AspMet: 1.257 ± 0.018	2.046AspAsn: 2.046 ± 0.021	3.116AspPro: 3.116 ± 0.027	1.738AspGln: 1.738 ± 0.019	2.862AspArg: 2.862 ± 0.032	4.249AspSer: 4.249 ± 0.037	3.036AspThr: 3.036 ± 0.029	3.845AspVal: 3.845 ± 0.035	0.844AspTrp: 0.844 ± 0.016	1.652AspTyr: 1.652 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	5.809GluAla: 5.809 ± 0.049	0.61GluCys: 0.61 ± 0.014	4.334GluAsp: 4.334 ± 0.046	5.831GluGlu: 5.831 ± 0.063	1.995GluPhe: 1.995 ± 0.023	4.308GluGly: 4.308 ± 0.035	1.267GluHis: 1.267 ± 0.018	3.172GluIle: 3.172 ± 0.03	3.89GluLys: 3.89 ± 0.042	5.208GluLeu: 5.208 ± 0.043	1.502GluMet: 1.502 ± 0.019	2.306GluAsn: 2.306 ± 0.024	2.723GluPro: 2.723 ± 0.043	2.334GluGln: 2.334 ± 0.033	3.917GluArg: 3.917 ± 0.045	4.363GluSer: 4.363 ± 0.034	3.5GluThr: 3.5 ± 0.029	3.83GluVal: 3.83 ± 0.028	0.883GluTrp: 0.883 ± 0.015	1.73GluTyr: 1.73 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.034PheAla: 3.034 ± 0.029	0.523PheCys: 0.523 ± 0.012	2.236PheAsp: 2.236 ± 0.023	2.17PheGlu: 2.17 ± 0.025	1.517PhePhe: 1.517 ± 0.022	2.945PheGly: 2.945 ± 0.033	0.803PheHis: 0.803 ± 0.013	1.812PheIle: 1.812 ± 0.028	1.61PheLys: 1.61 ± 0.02	3.199PheLeu: 3.199 ± 0.032	0.781PheMet: 0.781 ± 0.012	1.429PheAsn: 1.429 ± 0.017	1.847PhePro: 1.847 ± 0.021	1.361PheGln: 1.361 ± 0.019	1.772PheArg: 1.772 ± 0.02	2.872PheSer: 2.872 ± 0.027	2.155PheThr: 2.155 ± 0.025	2.325PheVal: 2.325 ± 0.023	0.619PheTrp: 0.619 ± 0.013	1.074PheTyr: 1.074 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.996GlyAla: 5.996 ± 0.053	0.881GlyCys: 0.881 ± 0.017	3.983GlyAsp: 3.983 ± 0.037	4.164GlyGlu: 4.164 ± 0.036	2.843GlyPhe: 2.843 ± 0.031	6.823GlyGly: 6.823 ± 0.08	1.706GlyHis: 1.706 ± 0.024	3.665GlyIle: 3.665 ± 0.031	3.852GlyLys: 3.852 ± 0.032	6.08GlyLeu: 6.08 ± 0.041	1.713GlyMet: 1.713 ± 0.023	2.796GlyAsn: 2.796 ± 0.034	3.371GlyPro: 3.371 ± 0.036	2.619GlyGln: 2.619 ± 0.031	4.141GlyArg: 4.141 ± 0.035	5.916GlySer: 5.916 ± 0.045	4.125GlyThr: 4.125 ± 0.036	4.689GlyVal: 4.689 ± 0.041	1.186GlyTrp: 1.186 ± 0.019	2.204GlyTyr: 2.204 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	1.741HisAla: 1.741 ± 0.023	0.287HisCys: 0.287 ± 0.009	1.234HisAsp: 1.234 ± 0.017	1.248HisGlu: 1.248 ± 0.017	0.858HisPhe: 0.858 ± 0.012	1.676HisGly: 1.676 ± 0.022	0.715HisHis: 0.715 ± 0.015	1.228HisIle: 1.228 ± 0.016	0.927HisLys: 0.927 ± 0.015	2.015HisLeu: 2.015 ± 0.025	0.446HisMet: 0.446 ± 0.01	0.844HisAsn: 0.844 ± 0.014	1.497HisPro: 1.497 ± 0.023	0.88HisGln: 0.88 ± 0.016	1.342HisArg: 1.342 ± 0.017	1.699HisSer: 1.699 ± 0.022	1.227HisThr: 1.227 ± 0.016	1.328HisVal: 1.328 ± 0.02	0.298HisTrp: 0.298 ± 0.008	0.673HisTyr: 0.673 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.438IleAla: 4.438 ± 0.038	0.723IleCys: 0.723 ± 0.015	2.972IleAsp: 2.972 ± 0.026	3.025IleGlu: 3.025 ± 0.028	2.006IlePhe: 2.006 ± 0.029	3.358IleGly: 3.358 ± 0.036	1.124IleHis: 1.124 ± 0.017	2.657IleIle: 2.657 ± 0.027	2.378IleLys: 2.378 ± 0.028	4.497IleLeu: 4.497 ± 0.042	1.055IleMet: 1.055 ± 0.018	1.884IleAsn: 1.884 ± 0.022	3.042IlePro: 3.042 ± 0.027	1.847IleGln: 1.847 ± 0.022	2.697IleArg: 2.697 ± 0.022	3.992IleSer: 3.992 ± 0.031	2.982IleThr: 2.982 ± 0.03	3.202IleVal: 3.202 ± 0.03	0.7IleTrp: 0.7 ± 0.013	1.454IleTyr: 1.454 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	4.657LysAla: 4.657 ± 0.04	0.502LysCys: 0.502 ± 0.014	2.95LysAsp: 2.95 ± 0.029	3.653LysGlu: 3.653 ± 0.041	1.558LysPhe: 1.558 ± 0.019	3.328LysGly: 3.328 ± 0.034	1.1LysHis: 1.1 ± 0.017	2.391LysIle: 2.391 ± 0.026	3.568LysLys: 3.568 ± 0.062	4.258LysLeu: 4.258 ± 0.033	1.062LysMet: 1.062 ± 0.017	1.861LysAsn: 1.861 ± 0.022	2.8LysPro: 2.8 ± 0.032	1.822LysGln: 1.822 ± 0.021	3.358LysArg: 3.358 ± 0.033	3.751LysSer: 3.751 ± 0.036	2.985LysThr: 2.985 ± 0.031	3.062LysVal: 3.062 ± 0.024	0.704LysTrp: 0.704 ± 0.014	1.437LysTyr: 1.437 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	7.748LeuAla: 7.748 ± 0.045	1.106LeuCys: 1.106 ± 0.015	5.05LeuAsp: 5.05 ± 0.04	5.402LeuGlu: 5.402 ± 0.044	3.133LeuPhe: 3.133 ± 0.028	5.94LeuGly: 5.94 ± 0.047	2.06LeuHis: 2.06 ± 0.022	3.945LeuIle: 3.945 ± 0.038	4.376LeuLys: 4.376 ± 0.041	8.122LeuLeu: 8.122 ± 0.06	1.721LeuMet: 1.721 ± 0.022	3.133LeuAsn: 3.133 ± 0.031	5.346LeuPro: 5.346 ± 0.041	3.643LeuGln: 3.643 ± 0.037	5.348LeuArg: 5.348 ± 0.043	7.071LeuSer: 7.071 ± 0.045	4.749LeuThr: 4.749 ± 0.033	5.232LeuVal: 5.232 ± 0.048	1.168LeuTrp: 1.168 ± 0.02	2.289LeuTyr: 2.289 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.252MetAla: 2.252 ± 0.025	0.229MetCys: 0.229 ± 0.008	1.28MetAsp: 1.28 ± 0.016	1.33MetGlu: 1.33 ± 0.019	0.745MetPhe: 0.745 ± 0.013	1.569MetGly: 1.569 ± 0.024	0.465MetHis: 0.465 ± 0.012	0.963MetIle: 0.963 ± 0.015	1.077MetLys: 1.077 ± 0.017	1.809MetLeu: 1.809 ± 0.022	0.576MetMet: 0.576 ± 0.012	0.773MetAsn: 0.773 ± 0.014	1.205MetPro: 1.205 ± 0.017	0.829MetGln: 0.829 ± 0.015	1.259MetArg: 1.259 ± 0.016	1.858MetSer: 1.858 ± 0.02	1.223MetThr: 1.223 ± 0.016	1.323MetVal: 1.323 ± 0.018	0.256MetTrp: 0.256 ± 0.009	0.532MetTyr: 0.532 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.283AsnAla: 3.283 ± 0.035	0.444AsnCys: 0.444 ± 0.012	2.027AsnAsp: 2.027 ± 0.025	2.076AsnGlu: 2.076 ± 0.025	1.421AsnPhe: 1.421 ± 0.018	3.228AsnGly: 3.228 ± 0.034	0.828AsnHis: 0.828 ± 0.015	2.191AsnIle: 2.191 ± 0.021	1.653AsnLys: 1.653 ± 0.023	3.213AsnLeu: 3.213 ± 0.03	0.86AsnMet: 0.86 ± 0.015	1.603AsnAsn: 1.603 ± 0.03	2.427AsnPro: 2.427 ± 0.026	1.318AsnGln: 1.318 ± 0.019	1.913AsnArg: 1.913 ± 0.02	2.932AsnSer: 2.932 ± 0.034	2.251AsnThr: 2.251 ± 0.024	2.301AsnVal: 2.301 ± 0.025	0.575AsnTrp: 0.575 ± 0.014	1.109AsnTyr: 1.109 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.317ProAla: 5.317 ± 0.049	0.463ProCys: 0.463 ± 0.012	3.029ProAsp: 3.029 ± 0.025	3.708ProGlu: 3.708 ± 0.034	1.925ProPhe: 1.925 ± 0.025	3.929ProGly: 3.929 ± 0.036	1.233ProHis: 1.233 ± 0.019	2.581ProIle: 2.581 ± 0.026	2.862ProLys: 2.862 ± 0.031	4.638ProLeu: 4.638 ± 0.034	1.034ProMet: 1.034 ± 0.018	2.248ProAsn: 2.248 ± 0.024	4.906ProPro: 4.906 ± 0.061	2.458ProGln: 2.458 ± 0.038	3.261ProArg: 3.261 ± 0.036	5.656ProSer: 5.656 ± 0.053	4.186ProThr: 4.186 ± 0.042	3.548ProVal: 3.548 ± 0.034	0.69ProTrp: 0.69 ± 0.013	1.479ProTyr: 1.479 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	3.35GlnAla: 3.35 ± 0.034	0.412GlnCys: 0.412 ± 0.011	1.959GlnAsp: 1.959 ± 0.026	2.268GlnGlu: 2.268 ± 0.022	1.254GlnPhe: 1.254 ± 0.018	2.529GlnGly: 2.529 ± 0.03	0.957GlnHis: 0.957 ± 0.017	1.849GlnIle: 1.849 ± 0.022	1.96GlnLys: 1.96 ± 0.022	3.316GlnLeu: 3.316 ± 0.033	0.847GlnMet: 0.847 ± 0.015	1.566GlnAsn: 1.566 ± 0.022	2.412GlnPro: 2.412 ± 0.035	2.435GlnGln: 2.435 ± 0.057	2.471GlnArg: 2.471 ± 0.029	2.976GlnSer: 2.976 ± 0.031	2.242GlnThr: 2.242 ± 0.023	2.081GlnVal: 2.081 ± 0.022	0.546GlnTrp: 0.546 ± 0.011	1.179GlnTyr: 1.179 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.697ArgAla: 4.697 ± 0.032	0.648ArgCys: 0.648 ± 0.014	3.31ArgAsp: 3.31 ± 0.034	3.807ArgGlu: 3.807 ± 0.042	1.909ArgPhe: 1.909 ± 0.017	3.896ArgGly: 3.896 ± 0.041	1.413ArgHis: 1.413 ± 0.02	2.77ArgIle: 2.77 ± 0.026	3.31ArgLys: 3.31 ± 0.032	5.033ArgLeu: 5.033 ± 0.041	1.241ArgMet: 1.241 ± 0.017	2.222ArgAsn: 2.222 ± 0.026	3.219ArgPro: 3.219 ± 0.033	2.474ArgGln: 2.474 ± 0.033	4.721ArgArg: 4.721 ± 0.048	4.466ArgSer: 4.466 ± 0.043	3.116ArgThr: 3.116 ± 0.028	3.244ArgVal: 3.244 ± 0.031	0.825ArgTrp: 0.825 ± 0.017	1.521ArgTyr: 1.521 ± 0.02	0.0ArgXaa: 0.0 ± 0.0
Ser	6.723SerAla: 6.723 ± 0.042	0.824SerCys: 0.824 ± 0.017	4.307SerAsp: 4.307 ± 0.038	4.171SerGlu: 4.171 ± 0.038	2.889SerPhe: 2.889 ± 0.028	5.863SerGly: 5.863 ± 0.047	1.776SerHis: 1.776 ± 0.021	4.062SerIle: 4.062 ± 0.033	3.953SerLys: 3.953 ± 0.035	6.947SerLeu: 6.947 ± 0.05	1.7SerMet: 1.7 ± 0.024	3.116SerAsn: 3.116 ± 0.029	5.422SerPro: 5.422 ± 0.051	3.133SerGln: 3.133 ± 0.029	4.682SerArg: 4.682 ± 0.046	8.198SerSer: 8.198 ± 0.072	5.499SerThr: 5.499 ± 0.052	4.525SerVal: 4.525 ± 0.034	1.071SerTrp: 1.071 ± 0.016	2.103SerTyr: 2.103 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	5.379ThrAla: 5.379 ± 0.04	0.715ThrCys: 0.715 ± 0.015	2.959ThrAsp: 2.959 ± 0.027	3.279ThrGlu: 3.279 ± 0.033	2.207ThrPhe: 2.207 ± 0.023	4.333ThrGly: 4.333 ± 0.044	1.228ThrHis: 1.228 ± 0.017	3.207ThrIle: 3.207 ± 0.032	2.806ThrLys: 2.806 ± 0.026	5.158ThrLeu: 5.158 ± 0.041	1.229ThrMet: 1.229 ± 0.016	2.204ThrAsn: 2.204 ± 0.024	4.41ThrPro: 4.41 ± 0.04	2.05ThrGln: 2.05 ± 0.025	2.984ThrArg: 2.984 ± 0.027	5.265ThrSer: 5.265 ± 0.038	4.473ThrThr: 4.473 ± 0.047	3.792ThrVal: 3.792 ± 0.029	0.836ThrTrp: 0.836 ± 0.016	1.647ThrTyr: 1.647 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.687ValAla: 5.687 ± 0.042	0.768ValCys: 0.768 ± 0.016	3.878ValAsp: 3.878 ± 0.037	4.174ValGlu: 4.174 ± 0.036	2.343ValPhe: 2.343 ± 0.025	4.291ValGly: 4.291 ± 0.043	1.299ValHis: 1.299 ± 0.018	2.994ValIle: 2.994 ± 0.027	3.109ValLys: 3.109 ± 0.03	5.48ValLeu: 5.48 ± 0.045	1.312ValMet: 1.312 ± 0.019	2.247ValAsn: 2.247 ± 0.025	3.52ValPro: 3.52 ± 0.029	2.247ValGln: 2.247 ± 0.027	3.259ValArg: 3.259 ± 0.032	4.504ValSer: 4.504 ± 0.034	3.55ValThr: 3.55 ± 0.032	4.562ValVal: 4.562 ± 0.043	0.841ValTrp: 0.841 ± 0.016	1.694ValTyr: 1.694 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.177TrpAla: 1.177 ± 0.018	0.18TrpCys: 0.18 ± 0.008	0.911TrpAsp: 0.911 ± 0.017	0.846TrpGlu: 0.846 ± 0.016	0.507TrpPhe: 0.507 ± 0.012	0.984TrpGly: 0.984 ± 0.017	0.332TrpHis: 0.332 ± 0.009	0.72TrpIle: 0.72 ± 0.013	0.775TrpLys: 0.775 ± 0.015	1.241TrpLeu: 1.241 ± 0.018	0.348TrpMet: 0.348 ± 0.009	0.608TrpAsn: 0.608 ± 0.012	0.59TrpPro: 0.59 ± 0.013	0.504TrpGln: 0.504 ± 0.011	0.858TrpArg: 0.858 ± 0.015	1.01TrpSer: 1.01 ± 0.017	0.899TrpThr: 0.899 ± 0.016	0.899TrpVal: 0.899 ± 0.016	0.269TrpTrp: 0.269 ± 0.008	0.405TrpTyr: 0.405 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.169TyrAla: 2.169 ± 0.024	0.406TyrCys: 0.406 ± 0.01	1.701TyrAsp: 1.701 ± 0.019	1.572TyrGlu: 1.572 ± 0.02	1.182TyrPhe: 1.182 ± 0.02	2.178TyrGly: 2.178 ± 0.025	0.71TyrHis: 0.71 ± 0.014	1.434TyrIle: 1.434 ± 0.017	1.189TyrLys: 1.189 ± 0.016	2.575TyrLeu: 2.575 ± 0.023	0.595TyrMet: 0.595 ± 0.011	1.195TyrAsn: 1.195 ± 0.018	1.454TyrPro: 1.454 ± 0.021	1.077TyrGln: 1.077 ± 0.017	1.507TyrArg: 1.507 ± 0.02	2.115TyrSer: 2.115 ± 0.023	1.66TyrThr: 1.66 ± 0.022	1.586TyrVal: 1.586 ± 0.022	0.42TyrTrp: 0.42 ± 0.01	0.933TyrTyr: 0.933 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8539 proteins (4315648 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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