Paraglaciecola hydrolytica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Paraglaciecola

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A136A2T0|A0A136A2T0_9ALTE Acyl-CoA desaturase OS=Paraglaciecola hydrolytica OX=1799789 GN=AX660_05550 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.14KK3 pKa = 9.38YY4 pKa = 10.17ALSIGMVSLLGCVSAQADD22 pKa = 4.13TLLGLYY28 pKa = 10.38AGAQGWNMEE37 pKa = 4.62TIGGFSDD44 pKa = 4.38DD45 pKa = 3.62GTNIAFNFEE54 pKa = 4.72DD55 pKa = 3.5KK56 pKa = 10.15TQGSFYY62 pKa = 10.98VAFEE66 pKa = 4.17HH67 pKa = 7.19PIPLVPNLKK76 pKa = 9.24VQRR79 pKa = 11.84TGMDD83 pKa = 3.14TDD85 pKa = 4.15GKK87 pKa = 11.14VSLTTSFTFGDD98 pKa = 3.89EE99 pKa = 4.27LFTANSTALTDD110 pKa = 3.65IKK112 pKa = 10.48LTSTDD117 pKa = 3.41AILYY121 pKa = 10.31YY122 pKa = 10.69EE123 pKa = 5.34LFDD126 pKa = 4.37NDD128 pKa = 5.33LISFDD133 pKa = 3.48VGINAKK139 pKa = 10.23YY140 pKa = 10.53LDD142 pKa = 4.21GEE144 pKa = 4.5LFVIDD149 pKa = 4.93SEE151 pKa = 4.81DD152 pKa = 3.48PSTSGRR158 pKa = 11.84EE159 pKa = 3.97EE160 pKa = 3.93FSGVVPMVYY169 pKa = 10.16SRR171 pKa = 11.84VAIGIPTTGLGFYY184 pKa = 10.26IEE186 pKa = 4.79GSFLSFDD193 pKa = 3.94DD194 pKa = 3.87QTLTDD199 pKa = 3.72YY200 pKa = 10.92QAALTYY206 pKa = 11.23SLMEE210 pKa = 4.22NLAIDD215 pKa = 3.48MTFQLGYY222 pKa = 10.41RR223 pKa = 11.84SVEE226 pKa = 3.75VDD228 pKa = 4.49LDD230 pKa = 4.17DD231 pKa = 6.29LDD233 pKa = 5.75GIYY236 pKa = 10.92SNLEE240 pKa = 3.68FKK242 pKa = 10.95GAFAGLEE249 pKa = 3.89LHH251 pKa = 7.11FF252 pKa = 5.38

Molecular weight: 27.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A136A4A8|A0A136A4A8_9ALTE Dipeptide epimerase OS=Paraglaciecola hydrolytica OX=1799789 GN=AX660_08585 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.48VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4354

0

4354

1486281

44

2403

341.4

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.795 ± 0.034

0.982 ± 0.013

5.51 ± 0.036

5.596 ± 0.032

4.333 ± 0.023

6.594 ± 0.039

2.212 ± 0.017

6.436 ± 0.028

5.321 ± 0.035

10.764 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.018

4.656 ± 0.026

3.824 ± 0.02

5.208 ± 0.035

4.099 ± 0.026

6.815 ± 0.031

5.248 ± 0.027

6.683 ± 0.024

1.318 ± 0.014

3.17 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski