Candidatus Nitrosocosmicus franklandus

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; Candidatus Nitrosocosmicus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484I8D6|A0A484I8D6_9ARCH Acetyl-coenzyme A synthetase OS=Candidatus Nitrosocosmicus franklandus OX=1798806 GN=acsA PE=3 SV=1
MM1 pKa = 7.4SLHH4 pKa = 6.33NEE6 pKa = 3.69NMIKK10 pKa = 10.24EE11 pKa = 4.29FSLLLILGILIVTSSTSMSNPIDD34 pKa = 3.81AQLNSSDD41 pKa = 3.91TNIGNNGVMSIGSSSGFSNTNTTGMGIDD69 pKa = 5.87DD70 pKa = 4.01IDD72 pKa = 3.52SWDD75 pKa = 3.66VVSGNWINRR84 pKa = 11.84AFTIQGGLPEE94 pKa = 4.22NSGLNRR100 pKa = 11.84IILYY104 pKa = 9.76PEE106 pKa = 4.24SGDD109 pKa = 3.48NFVKK113 pKa = 10.5ISTSFKK119 pKa = 10.62VNNLNSSTYY128 pKa = 8.43NYY130 pKa = 10.97ASIVYY135 pKa = 10.15SYY137 pKa = 11.28LNPDD141 pKa = 3.05NFKK144 pKa = 10.91LVGINIHH151 pKa = 7.04DD152 pKa = 3.97NAVYY156 pKa = 10.43AIGFTMRR163 pKa = 11.84NGIEE167 pKa = 4.1SAEE170 pKa = 4.18PFWPGVPTNLTWSPGTTYY188 pKa = 11.36NLTMVKK194 pKa = 9.92QGSSLDD200 pKa = 3.76LLVNDD205 pKa = 4.9TKK207 pKa = 11.54YY208 pKa = 9.85LTQNNSDD215 pKa = 3.4NQTNFGDD222 pKa = 3.62VGVSYY227 pKa = 11.17GRR229 pKa = 11.84INNITFFDD237 pKa = 4.48FEE239 pKa = 4.73TEE241 pKa = 3.82ITDD244 pKa = 4.22QSVNSNNSQRR254 pKa = 11.84NTNPGPFTTSDD265 pKa = 3.16SQAILLEE272 pKa = 4.73GNSLPEE278 pKa = 3.67NEE280 pKa = 4.49YY281 pKa = 11.0LHH283 pKa = 7.31LYY285 pKa = 7.78DD286 pKa = 3.47TTPYY290 pKa = 9.9RR291 pKa = 11.84IEE293 pKa = 4.44SGHH296 pKa = 5.86VAAKK300 pKa = 9.93LPCGEE305 pKa = 4.97DD306 pKa = 3.43NMPEE310 pKa = 4.55AILLAGQAPTLAPIDD325 pKa = 3.87LEE327 pKa = 4.43FVNEE331 pKa = 3.9LSIPDD336 pKa = 4.01EE337 pKa = 4.2MCLYY341 pKa = 10.35HH342 pKa = 8.45ADD344 pKa = 3.77INSSNIDD351 pKa = 4.48PITDD355 pKa = 3.07IAIANNSTDD364 pKa = 4.12EE365 pKa = 3.95IDD367 pKa = 3.84FPEE370 pKa = 4.25TSGIVISISEE380 pKa = 3.81ISMIEE385 pKa = 4.5PIWMDD390 pKa = 3.57ASQTTLDD397 pKa = 3.53NN398 pKa = 4.0

Molecular weight: 43.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484I7N5|A0A484I7N5_9ARCH Uncharacterized protein OS=Candidatus Nitrosocosmicus franklandus OX=1798806 GN=NFRAN_0439 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84CSKK6 pKa = 9.69CQKK9 pKa = 10.12GLHH12 pKa = 4.73TTYY15 pKa = 10.52IRR17 pKa = 11.84LGPKK21 pKa = 9.95AVFTKK26 pKa = 10.66LGYY29 pKa = 10.56YY30 pKa = 10.17CINCKK35 pKa = 10.38SYY37 pKa = 11.13TEE39 pKa = 4.37NNSQAFRR46 pKa = 11.84GARR49 pKa = 11.84KK50 pKa = 7.68QTQNRR55 pKa = 11.84MLRR58 pKa = 11.84PGFEE62 pKa = 4.16PGIVALRR69 pKa = 11.84GRR71 pKa = 11.84NAA73 pKa = 3.28

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3135

0

3135

684365

32

1264

218.3

24.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.023 ± 0.05

1.035 ± 0.018

5.66 ± 0.037

6.155 ± 0.043

4.52 ± 0.039

6.026 ± 0.052

1.826 ± 0.022

9.435 ± 0.053

7.427 ± 0.052

9.223 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.026

6.411 ± 0.045

3.633 ± 0.032

3.083 ± 0.033

3.998 ± 0.039

8.028 ± 0.048

5.482 ± 0.042

6.066 ± 0.04

0.888 ± 0.015

3.65 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski