Botryotinia calthae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botryotinia

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8D4R0|A0A4Y8D4R0_9HELO Uncharacterized protein OS=Botryotinia calthae OX=38488 GN=BOTCAL_0125g00260 PE=4 SV=1
MM1 pKa = 7.45SGYY4 pKa = 10.96GNEE7 pKa = 4.28EE8 pKa = 3.66QSDD11 pKa = 4.56SYY13 pKa = 11.54GGQSGNYY20 pKa = 9.08SGKK23 pKa = 9.8QEE25 pKa = 3.89SDD27 pKa = 3.52YY28 pKa = 11.29EE29 pKa = 4.29SRR31 pKa = 11.84DD32 pKa = 3.09NDD34 pKa = 4.12TQSGGYY40 pKa = 9.89GSARR44 pKa = 11.84NNYY47 pKa = 8.41TSGGLGGDD55 pKa = 4.62DD56 pKa = 4.92DD57 pKa = 6.64DD58 pKa = 5.78NDD60 pKa = 3.98NEE62 pKa = 4.62SGDD65 pKa = 4.09AYY67 pKa = 10.74GSTTEE72 pKa = 4.81DD73 pKa = 4.07GYY75 pKa = 11.83GSTRR79 pKa = 11.84KK80 pKa = 10.02DD81 pKa = 3.41DD82 pKa = 3.69TSGGLNNDD90 pKa = 3.78SFSSGDD96 pKa = 3.38TYY98 pKa = 11.58GSTRR102 pKa = 11.84GDD104 pKa = 3.25KK105 pKa = 10.95YY106 pKa = 11.36GSTGKK111 pKa = 10.29DD112 pKa = 2.97DD113 pKa = 3.7TTSGFGQTNTSSEE126 pKa = 4.12DD127 pKa = 3.57AYY129 pKa = 10.91GSTGNNDD136 pKa = 2.79TRR138 pKa = 11.84SGFGQDD144 pKa = 2.94NTTSEE149 pKa = 4.26DD150 pKa = 3.87TYY152 pKa = 11.73GSIGRR157 pKa = 11.84DD158 pKa = 2.97NTTSDD163 pKa = 3.42DD164 pKa = 3.77TYY166 pKa = 11.42SRR168 pKa = 11.84SRR170 pKa = 11.84KK171 pKa = 9.77DD172 pKa = 3.54DD173 pKa = 3.33TSSSYY178 pKa = 11.86NNDD181 pKa = 2.65ATFSGGAFGSTIGAGSDD198 pKa = 3.45SYY200 pKa = 12.18DD201 pKa = 3.34NDD203 pKa = 3.38KK204 pKa = 11.41VGGYY208 pKa = 9.59EE209 pKa = 4.13GVKK212 pKa = 10.29AGSSGYY218 pKa = 10.84GDD220 pKa = 5.48DD221 pKa = 3.65VTGDD225 pKa = 3.54SDD227 pKa = 3.97VSGGYY232 pKa = 10.34GRR234 pKa = 11.84DD235 pKa = 3.67KK236 pKa = 11.22NDD238 pKa = 3.28SQSLGGSGDD247 pKa = 3.52NALSGGYY254 pKa = 10.27GGDD257 pKa = 3.92DD258 pKa = 3.58NQISSGYY265 pKa = 9.88GAGDD269 pKa = 3.44YY270 pKa = 11.05DD271 pKa = 6.26GNDD274 pKa = 3.45QTNSGGYY281 pKa = 9.88GDD283 pKa = 5.3NEE285 pKa = 3.99QSQSGLKK292 pKa = 10.1KK293 pKa = 10.04GLEE296 pKa = 3.99IAKK299 pKa = 9.84EE300 pKa = 4.12LYY302 pKa = 9.93HH303 pKa = 7.12KK304 pKa = 10.81NN305 pKa = 3.23

Molecular weight: 31.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8CQS2|A0A4Y8CQS2_9HELO Uncharacterized protein OS=Botryotinia calthae OX=38488 GN=BOTCAL_0447g00050 PE=4 SV=1
MM1 pKa = 7.11ATAEE5 pKa = 4.34PSPASSSNRR14 pKa = 11.84PPRR17 pKa = 11.84TRR19 pKa = 11.84TRR21 pKa = 11.84RR22 pKa = 11.84PRR24 pKa = 11.84RR25 pKa = 11.84GGHH28 pKa = 6.46RR29 pKa = 11.84NTGPAPADD37 pKa = 3.43ANTNATPNQTTQAPNGPLALRR58 pKa = 11.84PVSVASPPGSNPSTPSNPRR77 pKa = 11.84NSNANNRR84 pKa = 11.84GGGNGRR90 pKa = 11.84RR91 pKa = 11.84GGFGRR96 pKa = 11.84GGRR99 pKa = 11.84RR100 pKa = 11.84GGGRR104 pKa = 11.84GQPMANGRR112 pKa = 11.84VFGGQLTSNLHH123 pKa = 5.78TGPTSDD129 pKa = 4.43AGSLAGNAPAFTPGQPIAARR149 pKa = 11.84PGPSRR154 pKa = 11.84APRR157 pKa = 11.84ARR159 pKa = 11.84RR160 pKa = 11.84MSKK163 pKa = 9.68SQAADD168 pKa = 3.17LPTRR172 pKa = 11.84THH174 pKa = 6.58EE175 pKa = 5.44DD176 pKa = 2.94ITNGQYY182 pKa = 10.63EE183 pKa = 4.4CAICTNEE190 pKa = 3.78VLPNSKK196 pKa = 8.8IWNCKK201 pKa = 7.95SCWSVLHH208 pKa = 6.05LSCVKK213 pKa = 10.01KK214 pKa = 9.29WSKK217 pKa = 11.41NEE219 pKa = 3.82VSTHH223 pKa = 3.98QQRR226 pKa = 11.84AVEE229 pKa = 4.27NGEE232 pKa = 4.26LPPPRR237 pKa = 11.84QWRR240 pKa = 11.84CPGCNLPKK248 pKa = 10.16EE249 pKa = 4.57DD250 pKa = 4.92LPISYY255 pKa = 8.33TCWCEE260 pKa = 3.94KK261 pKa = 10.23EE262 pKa = 4.57LGYY265 pKa = 10.65HH266 pKa = 6.73HH267 pKa = 7.14ILVGKK272 pKa = 8.93VVQKK276 pKa = 10.96SGLDD280 pKa = 3.28TVLIHH285 pKa = 7.46AISCAMLDD293 pKa = 3.66RR294 pKa = 11.84VHH296 pKa = 7.06LALIWGLHH304 pKa = 5.21FLASVAKK311 pKa = 10.29RR312 pKa = 11.84RR313 pKa = 11.84FQEE316 pKa = 4.24DD317 pKa = 3.1VWIRR321 pKa = 11.84STKK324 pKa = 10.33ADD326 pKa = 3.34GVVGKK331 pKa = 9.35FAMMYY336 pKa = 9.79FPAEE340 pKa = 3.89KK341 pKa = 9.5TIVSEE346 pKa = 4.26VVMRR350 pKa = 11.84ASEE353 pKa = 4.2AVVKK357 pKa = 10.13FLLNQHH363 pKa = 6.68AIVVKK368 pKa = 10.29SRR370 pKa = 11.84KK371 pKa = 9.5LYY373 pKa = 9.74LARR376 pKa = 11.84NAMKK380 pKa = 10.24SVKK383 pKa = 9.73ARR385 pKa = 11.84HH386 pKa = 4.31QPIRR390 pKa = 11.84LMVKK394 pKa = 8.56VWPRR398 pKa = 11.84EE399 pKa = 3.75HH400 pKa = 6.91GLDD403 pKa = 3.54HH404 pKa = 6.66LTVALNAVDD413 pKa = 4.33PLIAVNRR420 pKa = 11.84IITVRR425 pKa = 11.84LDD427 pKa = 3.36ATLKK431 pKa = 9.62TYY433 pKa = 10.63YY434 pKa = 10.4LHH436 pKa = 6.67TVHH439 pKa = 6.94CRR441 pKa = 11.84QTLSQLVPAEE451 pKa = 3.98KK452 pKa = 10.32RR453 pKa = 11.84QFLHH457 pKa = 6.1SSKK460 pKa = 9.42PHH462 pKa = 5.8EE463 pKa = 5.16PIAPSIFLTVKK474 pKa = 10.36RR475 pKa = 11.84SANCRR480 pKa = 3.45

Molecular weight: 52.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12263

0

12263

5604297

21

6720

457.0

50.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.634 ± 0.017

1.194 ± 0.009

5.559 ± 0.018

6.558 ± 0.028

3.758 ± 0.012

6.715 ± 0.024

2.274 ± 0.01

5.562 ± 0.015

5.481 ± 0.024

8.558 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.008

4.296 ± 0.012

5.714 ± 0.026

3.879 ± 0.017

5.617 ± 0.022

8.814 ± 0.03

6.169 ± 0.019

5.754 ± 0.016

1.388 ± 0.009

2.838 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski