Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Dipodascaceae; Yarrowia; Yarrowia lipolytica

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6C8J5|Q6C8J5_YARLI 18S rRNA aminocarboxypropyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=TSR3 PE=3 SV=1
MM1 pKa = 7.55NYY3 pKa = 10.37SAFYY7 pKa = 10.49DD8 pKa = 3.69SSPSFQTKK16 pKa = 9.51SMNYY20 pKa = 9.26PPDD23 pKa = 3.75SVCPMDD29 pKa = 5.89LFPKK33 pKa = 9.64PVNFAPEE40 pKa = 4.84EE41 pKa = 4.08IPSFNILEE49 pKa = 4.11PNYY52 pKa = 11.06AFDD55 pKa = 4.87NDD57 pKa = 4.1SQSTLTGDD65 pKa = 3.51EE66 pKa = 4.21TDD68 pKa = 5.13FDD70 pKa = 4.3ALSDD74 pKa = 3.57VDD76 pKa = 5.05SEE78 pKa = 5.08YY79 pKa = 10.39EE80 pKa = 4.09VNYY83 pKa = 9.83VRR85 pKa = 11.84HH86 pKa = 5.57EE87 pKa = 4.43SKK89 pKa = 10.71LGEE92 pKa = 4.23EE93 pKa = 4.16NFEE96 pKa = 4.43DD97 pKa = 5.29DD98 pKa = 4.46EE99 pKa = 4.97LSEE102 pKa = 4.55DD103 pKa = 3.58EE104 pKa = 5.56HH105 pKa = 7.7YY106 pKa = 10.84LPHH109 pKa = 6.45YY110 pKa = 8.89LSPVAIQQQGKK121 pKa = 9.1NVGLVKK127 pKa = 10.23RR128 pKa = 11.84QHH130 pKa = 5.52VRR132 pKa = 11.84NSRR135 pKa = 11.84PRR137 pKa = 11.84DD138 pKa = 3.21LDD140 pKa = 3.15QFEE143 pKa = 4.66ILVVTPAYY151 pKa = 10.55SPEE154 pKa = 4.09EE155 pKa = 4.17EE156 pKa = 4.29PLSPTSCYY164 pKa = 10.9VCTEE168 pKa = 4.19EE169 pKa = 5.44IGPVDD174 pKa = 5.03DD175 pKa = 4.7YY176 pKa = 11.08PSPGHH181 pKa = 6.45SPDD184 pKa = 5.32LGDD187 pKa = 4.74LTDD190 pKa = 6.05DD191 pKa = 4.41DD192 pKa = 4.55TTSDD196 pKa = 3.53TTLDD200 pKa = 3.49TLVEE204 pKa = 4.11HH205 pKa = 7.02SSFSEE210 pKa = 4.03DD211 pKa = 2.65LWFQRR216 pKa = 11.84KK217 pKa = 7.81PNEE220 pKa = 3.88MHH222 pKa = 6.78EE223 pKa = 4.3MM224 pKa = 3.86

Molecular weight: 25.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6BZR2|Q6BZR2_YARLI YALI0F31537p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=YALI0_F31537g PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6449

5

6454

3123758

10

6538

484.0

53.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.826 ± 0.032

1.207 ± 0.012

5.98 ± 0.025

6.52 ± 0.035

3.889 ± 0.02

6.385 ± 0.033

2.385 ± 0.014

4.941 ± 0.025

5.901 ± 0.034

8.716 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.013

4.042 ± 0.018

5.414 ± 0.03

4.192 ± 0.028

5.045 ± 0.025

8.241 ± 0.047

6.115 ± 0.041

6.731 ± 0.028

1.201 ± 0.011

3.005 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski